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Analysis of the archaeal sub-seafloor community at Suiyo Seamount on the Izu-Bonin Arc
Institution:1. Department of Molecular Biology, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan;2. Tohoku University, Graduate School of Science, Aramaki Aza, Aoba, Sendai 980-0845, Japan;3. Research Institute of Biological Resources, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan;4. Department of Earth Planetary Sciences, Faculty of Science, Kyushu University, 6-101 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan;5. National Institute of Advanced Industrial Science and Technology (AIST), 1-1-3 No. 7, Tsukuba, Ibaraki 305-8566, Japan;6. Earth and Planetary Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
Abstract:A sub-surface archaeal community at the Suiyo Seamount in the Western Pacific Ocean was investigated by 16S rRNA gene sequence and whole-cell in situ hybridization analyses. In this study, we drilled and cased holes at the hydrothermal area of the seamount to minimize contamination of the hydrothermal fluid in the sub-seafloor by penetrating seawater. PCR clone analysis of the hydrothermal fluid samples collected from a cased hole indicated the presence of chemolithoautotrophic primary biomass producers of Archaeoglobales and the Methanococcales-related archaeal HTE1 group, both of which can utilize hydrogen as an electron donor. We discuss the implication of the microbial community on the early history of life and on the search for extraterrestrial life.
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