Effects of space flight exposure on cell growth,tumorigenicity and gene expression in cancer cells |
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Authors: | Cheng Yang Yuehui LiZhijie Zhang Chen LuoYongqing Tong Guohua ZhouPingli Xie Jinyue HuGuancheng Li |
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Institution: | Cancer Research Institute, Xiangya Medical School, Central South University, Changsha 410078, China |
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Abstract: | It is well recognized that harsh outer space environment, consisting of microgravity and radiation, poses significant health risks for human cells. To investigate potential effects of the space environment exposure on cancer cells we examined the biological changes in Caski cells carried by the “Shen Zhou IV” spaceship. After exposure for 7 days in spaceflight, 1440 survival subclonal cell lines were established and 4 cell lines were screened. 44F10 and 17E3 were selected because of their increased cell proliferation and tumorigenesis, while 48A9 and 31F2 had slower cytological events. Experiments with cell proliferation assay, flow cytometry, soft agar assay, tumorigenesis assay and DNA microarray analysis have shown that selected cell lines presented multiple biological changes in cell morphology, cell growth, tumorigenicity and gene expression. These results suggest that space environment exposure can make significant biological impact on cancer cells and provide an entry point to find the immunological target of tumorigenesis. |
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Keywords: | Cervical carcinoma Differential gene expression DNA microarray |
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