Simulated microgravity inhibits cell wall regeneration of Penicillium decumbens protoplasts |
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Authors: | C Zhao Y SunZC Yi L RongFY Zhuang YB Fan |
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Institution: | School of Biological Science and Medical Engineering, Beihang University, Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, Haidian District Xueyuan Road 37#, Beijing 100191, China |
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Abstract: | This work compares cell wall regeneration from protoplasts of the fungus Penicillium decumbens under rotary culture (simulated microgravity) and stationary cultures. Using an optimized lytic enzyme mixture, protoplasts were successfully released with a yield of 5.3 × 105 cells/mL. Under simulated microgravity conditions, the protoplast regeneration efficiency was 33.8%, lower than 44.9% under stationary conditions. Laser scanning confocal microscopy gave direct evidence for reduced formation of polysaccharides under simulated conditions. Scanning electron microscopy showed the delayed process of cell wall regeneration by simulated microgravity. The delayed regeneration of P. decumbens cell wall under simulated microgravity was likely caused by the inhibition of polysaccharide synthesis. This research contributes to the understanding of how gravitational loads affect morphological and physiological processes of fungi. |
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Keywords: | Simulated microgravity Protoplast Cell wall Regeneration Polysaccharide |
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