Studying the visceral physiology of tadpoles through their naturally transparent abdominal walls.

We propose using anuran tadpoles with naturally transparent abdominal skin to study the visceral physiology of amphibian larvae under microgravity. The transparency of the abdominal wall in certain tadpoles enables one to evaluate the basal physiological state and temporal changes in viscera from their movements without any invasive treatment. In order to validate our experimental design, the intestinal motility and heart rate of Rhacophorus tadpoles were examined as indices of physiological responses to stepwise changes in temperature.