航天器AIT中心微生物多样性分析

航天器AIT（总装、集成和测试）中心的洁净度控制是防止造成航天器正向污染的重要保障.利用高通量测序技术与传统培养法比较分析了中国某航天器AIT中心的空气微生物组成与多样性.基于高通量的测序分析结果显示:该AIT中心空气中优势细菌以芽孢杆菌属为主，相对丰度78.47%±1.59%；优势真菌为银耳目，相对丰度8.97%±0.93%；基于培养法获得的优势细菌为葡萄球菌属，且未获得真菌培养物.空气微生物的chao1指数、Simpson多样性指数以及Shannon多样性指数分析显示，该AIT中心空气中细菌的多样性水平均高于真菌. 

Microbial Diversity Analysis in Spaceflight AIT Center

The assembly of spacecraft in clean room environment is essential for the prevention of forward contamination. In this study, both high-throughput sequencing and culture-based method are used to study the microbial community structure and diversity in the AIT (Assembly, Integration and Test) center of the spaceflight. The result of high-throughput sequencing analysis show that in the AIT center the dominant genus of airborne bacteria is Bacillus (78.47%±1.59%), and the dominant fungi is Tremellales (8.97%±0.93%). However, culture-based analysis indicates that the Staphylococcus is the dominant bacteria, and no fungal colony is observed in the AIT center. The microbial diversity is evaluated by chao1 index, Simpson index and Shannon index, and the results show that the bacterial diversity level is higher than that of fungal in the AIT center.