Molecular analysis of mutagenesis by high LET radiation. |
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| Authors: | T K Hei L X Zhu D Vannais C A Waldren |
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| Institution: | Center for Radiological Research, Columbia University, New York 10032, USA. |
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| Abstract: | Mutation induction by high linear energy transfer LET] alpha particles and gamma-rays was scored in the human hamster hybrid AL] cells. Southern blotting technique was used to analyse the molecular changes in the DNA from both the HGPRT- and S1- mutants. Dose dependent mutagenesis in the AL cells irradiated with the charged particles was higher by almost 20 fold at the S1 than the corresponding HGPRT locus. Southern analysis of the mutants induced by the high LET particles showed mostly multilocus deletion at both the HGPRT and S1 genes. |
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