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Detection of DNA damages and repair in human culture cells with simulated space radiation |
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| Authors: | S Nagaoka T Nakano S Endo T Onizuka Y Kagawa K Fujitaka K Ohnishi A Takahashi T Ohnishi |
| Institution: | 1 National Space Development Agency of Japan, Tsukuba 305, Japan 2 TORAY Research Center, Kamakura 248, Japan 3 National Institute for Radiological Sciences, Chiba 263, Japan 4 Nara Medical University, Nara 634, Japan |
| Abstract: | DNA damages and its repair of cultured WI38 human fibroblast cells and T98G human glioblastoma cells were studied by exposing to carbon ion beams of HIMAC accelerator. The exposed cells were incubated at 37 °C for appropriate intervals and the damages were analyzed by alkaline comet assay and quantitative RT-PCR with p53 mRNA Highly inhomogeneous DNA damages were observed among the electrophoretic cell images of the comet assay. The degree of the damages was analyzed semi-quantitatively by using the Comet Index. The damaged fraction of WI38 cells was 85% immediately after 4 Gy (100 keV/μm) irradiation and decreased to 50% after 120 min. incubation indicating a repair of cell DNA. Time dependent p53 gene expression was also analyzed by the quantitative RT-PCR method. |
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