| 空间培养箱中实时观察GFP标记开花基因表达方法与技术 |
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| 引用本文： | 王丽华, 谢俊燕, 张岳, 郑慧琼. 空间培养箱中实时观察GFP标记开花基因表达方法与技术[J]. 空间科学学报, 2016, 36(4): 562-565. doi: 10.11728/cjss2016.04.562 |
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| 作者姓名： | 王丽华 谢俊燕 张岳 郑慧琼 |
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| 作者单位： | 中国科学院上海生命科学研究院植物生理生态所 上海 200032 |
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| 基金项目： | 国家载人航天天宫二号工程项目;中国科学院战略性先导科技项目(XDA04020202);国家重点基础研究发展计划项目(2011CB710902)共同资助 |
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| 摘 要： | 绿色荧光蛋白（Green Fluorescent Protein，GFP）已被广泛用作一种强有力的生物发光报告蛋白.但是，其在空间植物培养箱中应用尚存很大困难.本文提出一种在空间植物培养箱中利用LED作为激发光源，对拟南芥中开花基因启动子控制下GFP基因表达的观察与分析方法，为在空间生物学实验中研究基因表达模式提供了新的方法和技术.
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| 关 键 词： | 空间培养箱 绿色荧光蛋白质 开花基因 拟南芥 |
| 收稿时间： | 2015-11-10 |
| 修稿时间： | 2016-05-09 |
Method and Technology of Monitoring the Expression of Flowering Gene Marked with Green Fluorescent Protein in Space Culture Chamber |
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| WANG Lihua, XIE Junyan, ZHANG Yue, ZHENG Huiqiong. Method and Technology of Monitoring the Expression of Flowering Gene Marked with Green Fluorescent Protein in Space Culture Chamber[J]. Chinese Journal of Space Science, 2016, 36(4): 562-565. doi: 10.11728/cjss2016.04.562 |
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| Authors: | WANG Lihua XIE Junyan ZHANG Yue ZHENG Huiqiong |
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| Affiliation: | Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032 |
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| Abstract: | Green fluorescent Protein (GFP) has been widely used as a powerful bio-luminescence reporter molecule for monitoring gene expression on ground. However, it is still difficult to use GFP as a tool in the space culture chamber for life studies. In this study, we present a technical method, which use the LED as excitation light source, for monitoring and analysis of GFP gene expression of the flowering locus T gene in Arabidopsis thaliana that is controlled by the promoter. The method in this study provides a new technical reference for the gene expression studies in space. |
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| Keywords: | Space culture chamber Green fluorescent protein Flowering locus T Arabidopsis |
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