Expression of estrogen receptor α in human breast cancer cells regulates mitochondrial oxidative stress under simulated microgravity |
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Authors: | Hong-xia Zheng Wei-ming Tian Hong-ji Yan Hua-dong Jiang Shan-shan Liu Lei Yue Fang Han Li-jun Wei Xiong-biao Chen Yu Li |
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Institution: | 1. Bio-X Center, School of Biological Science and Technology, Harbin Institute of Technology, Harbin, 150080, PR China;2. State Key Laboratory of Space Medicine Fundamentals and Application, Chinese Astronaut Research and Training Center, China;3. Department of Mechanical Engineering, University of Saskatchewan, Saskatoon, Canada |
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Abstract: | This study investigated intracellular oxidative stress and its underlying mechanisms in a rotary cell culture system used to achieve a simulated microgravity (SMG) environment. Experiments were conducted with human breast cancer cell lines MCF-7 (an estrogen receptor (ER) α positive cell line) and MDA-MB-231 (an ERα negative cell line) encapsulated in alginate/collagen carriers. After 48 h, SMG led to oxidative stress and DNA damage in the MDA-MB-231 cells but a significant increase in mitochondrial activity and minimal DNA damage in the MCF-7 cells. The activity of superoxide dismutase (SOD) significantly increased in the MCF-7 cells and decreased in MDA-MB-231 cells in the SMG environment compared with a standard gravity control. Moreover, SMG promoted expression of ERα and protein kinase C (PKC) epsilon in MCF-7 cells treated with PKC inhibitor Gö6983. Overall, exposure to SMG increased mitochondrial activity in ERα positive cells but induced cellular oxidative damage in ERα negative cells. Thus, ERα may play an important role in protecting cells from oxidative stress damage under simulated microgravity. |
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Keywords: | Oxidative stress Simulated microgravity Alginate/collagen carriers Oxidative stress Estrogen receptor α |
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