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EFFECT OF SURFACTANT SDS ON DETERMINATION OF NUCLEIC ACID WITH TERBIUM (III)FLUO
作者姓名:WuHui  WanYu
作者单位:CollegeofMaterialScienceandTechnology,NUAA29YudaoStreet,Nanjing210016,P.R.China
摘    要:The effect of an anionic surfactant(sodium dodecylsulfate.SDS)on the fluorescence properties of nucleic acid with terbium(III)is studied.Results show that ri-bonucleir acid (RNA)presents fluorescence reaction with Tb(III)directly.but deoxyribonucleic acid(DNA)pre-sents similar fluorescence reaction only after its denatura-tion.In the presence of SDS ,the fluorescence intensity is 4.0 times and 3.5 times greater than that of DNA and RNA without SDS.

关 键 词:fluorescence  properties  gurfactant  DNA  RNA  terbium  荧光性质  表面活性剂  十二烷基磺酸钠  核酸  DNA  RNA  
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