EFFECT OF SURFACTANT SDS ON DETERMINATION OF NUCLEIC ACID WITH TERBIUM (III)FLUO |
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作者姓名: | WuHui WanYu |
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作者单位: | CollegeofMaterialScienceandTechnology,NUAA29YudaoStreet,Nanjing210016,P.R.China |
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摘 要: | The effect of an anionic surfactant(sodium dodecylsulfate.SDS)on the fluorescence properties of nucleic acid with terbium(III)is studied.Results show that ri-bonucleir acid (RNA)presents fluorescence reaction with Tb(III)directly.but deoxyribonucleic acid(DNA)pre-sents similar fluorescence reaction only after its denatura-tion.In the presence of SDS ,the fluorescence intensity is 4.0 times and 3.5 times greater than that of DNA and RNA without SDS.
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关 键 词: | fluorescence properties gurfactant DNA RNA terbium 荧光性质 表面活性剂 十二烷基磺酸钠 核酸 DNA RNA 铽 |
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