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WuHui WanYu 《南京航空航天大学学报(英文版)》2002,19(1):94-97
The effect of an anionic surfactant(sodium dodecylsulfate.SDS)on the fluorescence properties of nucleic acid with terbium(III)is studied.Results show that ri-bonucleir acid (RNA)presents fluorescence reaction with Tb(III)directly.but deoxyribonucleic acid(DNA)pre-sents similar fluorescence reaction only after its denatura-tion.In the presence of SDS ,the fluorescence intensity is 4.0 times and 3.5 times greater than that of DNA and RNA without SDS. 相似文献
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研究了 SDS对核酸 -Tb3+体系荧光性质的影响。实验结果表明 ,RNA可以直接与 Tb3+ 反应产生荧光 ,而DNA只能在变性后才能与 Tb3+ 反应。SDS存在下 ,DNA与 RNA体系荧光强度分别增加 4.0和 3 .5倍。 相似文献
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