Molecular alterations in tumorigenic human bronchial and breast epithelial cells induced by high LET radiation.

Carcinogenesis is a multi-stage process with sequence of genetic events governing the phenotypic expression of a series of transformation steps leading to the development of metastatic cancer. In the present study, immortalized human bronchial (BEP2D) and breast (MCF-10F) cells were irradiated with graded doses of either 150 keV/micrometer alpha particles or 1 GeV/nucleon 56Fe ions. Transformed cells developed through a series of successive steps before becoming tumorigenic in nude mice. Cell fusion studies indicated that radiation-induced tumorigenic phenotype in BEP2D cells could be completely suppressed by fusion with non-tumorigenic BEP2D cells. The differential expressions of known genes between tumorigenic bronchial and breast cells induced by alpha particles and their respective control cultures were compared using cDNA expression array. Among the 11 genes identified to be differentially expressed in BEP2D cells, three (DCC, DNA-PK and p21(CIP1)) were shown to be consistently down-regulated by 2 to 4 fold in all the 5 tumor cell lines examined. In contrast, their expressions in the fusion cell lines were comparable to control BEP2D cells. Similarly, expression levels of a series of genes were found to be altered in a step-wise manner among tumorigenic MCF-10F cells. The results are highly suggestive that functional alterations of these genes may be causally related to the carcinogenic process.     

用于导弹系统性能统计分析的统计线性化伴随法

本文在研究ＳＬＡＭ方法基础上，进行了理论推导和计算方法方面的改进，并将此方法应用于导弹系统性能的统计分析，计算机仿真结果表明该方法与采用ＣＡＤＥＴ方法的结果完全一致。     

对提高统计线性化精度及高阶矩传播法的研究

提出提高统计线性化精度的新方法,同时研究高阶矩传播法的计算方法,并以此方法做为衡量计算精度的标准,计算结果表明,提出的修正方法改善了计算精度,并且易于推广使用。     

37kW底盘测功机计算机控制系统

底盘测功机是检验汽车、摩托车输出功率及其相关参数的一种检测设备。本课题利用现代先进的计算机控制技术实现对37kW-CHDY底盘测功机的行驶阻力控制、测试数据自动采集,从而实现摩托车道路工况模拟运转以及整车动力性、燃料经济性等指标的测量。     

典型加筋板的优化设计

对飞机结构设计中典型加筋板优化设计问题进行了研究 ,对典型加筋板形式、优化问题、计算方法和优化策略进行了描述 ,编写了优化设计程序 ,并进行了算例考核。计算结果表明 ,优化设计结果合理 ,可对该类加筋板的结构设计提供参考和指导。     

U型复合材料结构件热压罐固化变形及补偿技术应用研究

以复合材料U型构件为研究对象,采用数值模拟的方法对U型件进行热压罐温度场的模拟,模拟出在不同时间段工装和工件的温度分布情况,模拟工件在工装传热和变形影响下的内部传热、固化度、最终变形情况和残余应力水平等。根据模拟结果,优化热压罐固化工艺参数,从而改善固化热变形问题,根据分析结果制定补偿方案并实施验证,同时优化工装结构形式,采用工装型面补偿技术对零件固化变形进行预补偿。结果表明,补偿后零件变形后的外形与理论几何外形相差1.37mm,验证了补偿技术的可行性。工装型面补偿技术可以有效提高复合材料结构件的制造精度。     

铝合金厚板搅拌摩擦焊焊缝疏松缺陷形成机理

采用圆锥形搅拌头焊接20mm厚的7075-T6铝板,分析焊接过程中焊缝内部疏松缺陷的形成过程及原因。研究表明,焊缝表面成形良好,无明显缺陷。但是,在焊缝轴肩区和焊核区之间出现了疏松缺陷。分析认为,焊缝上、下部金属温度差太大,导致其塑性流动行为发生变化是疏松缺陷形成的主要原因。搅拌摩擦焊(FSW)过程中,焊缝上部金属温度较高,而底部温度仍然很低,脱离搅拌针端部的塑化金属在周围冷金属巨大的变形抗力作用下转而沿搅拌针表面往上迁移。到达轴肩区下方汇聚区时,由于轴肩区金属温度高,向下的挤压力太小,导致回迁上来的塑化金属继续往上迁移并冲破轴肩区而沿轴肩边缘溢出形成飞边。汇聚区内没有足够的塑化金属填充、焊缝无法被压实而产生疏松孔洞。通过建立疏松缺陷形成的物理模型,可以更直观地反映出焊缝金属流动形态及缺陷形成过程。     

Ionospheric scintillations at Guilin detected by GPS ground-based and radio occultation observations

The occurrence of ionospheric scintillations with S4 ? 0.2 was studied using GPS measurements at Guilin, China (25.29°N, 110.33°E; geomagnetic: 15.04°N, 181.98°E), a station located near the northern crest of the equatorial anomaly. The results are presented for data collected from January 2009 to March 2010. The results show that nighttime amplitude scintillations only took place in February and March of the considered years, while daytime amplitude scintillations occurred in August and December of 2009. Nighttime amplitude scintillations, observed in the south of Guilin, always occurred with phase scintillations, TEC (Total Electron Content) depletions, and ROT (Rate Of change of TEC) fluctuations. However, TEC depletions and ROT fluctuations were weak during daytime amplitude scintillations, and daytime amplitude scintillations always took place simultaneously for most of the GPS satellites which appeared over Guilin in different azimuth directions. Ground-based GPS scintillation/TEC observations recorded at Guilin and signal-to-noise-ratio (SNR) measurements obtained from GPS-COSMIC radio occultation indicate that nighttime and daytime scintillations are very likely caused by ionospheric F region irregularities and sporadic E, respectively. Moreover, strong daytime amplitude scintillations may be associated with the plasma density enhancements in ionospheric E region caused by the Perseid and Geminid meteor shower activities.     

Genomic instability and tumorigenic induction in immortalized human bronchial epithelial cells by heavy ions

Carcinogenesis is postulated to be a progressive multistage process characterized by an increase in genomic instability and clonal selection with each mutational event endowing a selective growth advantage. Genomic instability as manifested by the amplification of specific gene fragments is common among tumor and transformed cells. In the present study, immortalized human bronchial (BEP2D) cells were irradiated with graded doses of either 1GeV/nucleon ⁵⁶Fe ions or 150 keV/μm alpha particles. Transformed cells developed through a series of successive steps before becoming tumorigenic in nude mice. Tumorigenic cells showed neither ras mutations nor deletion in the p16 tumor suppressor gene. In contrast, they harbored mutations in the p53 gene and over-expressed cyclin D1. Genomic instability among transformed cells at various stage of the carcinogenic process was examined based on frequencies of PALA resistance. Incidence of genomic instability was highest among established tumor cell lines relative to transformed, non-tumorigenic and control cell lines. Treatment of BEP2D cells with a 4 mM dose of the aminothiol WR-1065 significantly reduced their neoplastic transforming response to ⁵⁶Fe particles. This model provides an opportunity to study the cellular and molecular mechanisms involved in malignant transformation of human epithelial cells by heavy ions.     

BTA 深孔钻削切屑变形与刀齿切削力分布规律仿真

采用ABAQUS有限元分析软件,建立了错齿BTA钻头钻削环状工件单元切削仿真模型,以45#钢为工件材料,对错齿BTA钻头各刀齿切削不同直径的环状工件进行了模拟仿真,分析了钻削过程中错齿BTA钻头各个刀齿切屑的形成过程与变形特点,研究了环状单元切削力三分量随工件半径的受力分布规律.结果表明,错齿BTA钻头各刀齿切削力分量呈现出随半径增大而增大的非均匀分布规律,并且切削速度和进给量对切削力分布规律几乎没有影响.