Gravity effects on connective tissue biosynthesis by cultured mesenchymal cells.

Quantitative and qualitative aspects of collagen synthesis under microgravity, normal gravity and hypergravity conditions were investigated during the spacelab D-2 mission by incubating human fibroblast cultures with [3H]-proline for 0, 4, 7, 10 and 20 hours. Quantitative analysis revealed an increase of collagen synthesis under microgravity conditions, being 40% higher than 1g controls. Hypergravity samples at 1.44g, 6.6g and 10g showed a decrease in collagen synthesis with increasing g, being down to about 15% at 10g. The relative proportion of collagen from total protein synthesized, the secretion of collagen by the cells, proline hydroxylation of individual collagen alpha-chains and the relative proportions of collagens I, III and V synthesized were not affected at any of the applied conditions.     

Simulation experiments of the effect of space environment on bacteriophage and DNA thin films.

The main goal of PUR experiment (phage and uracil response) is to examine and quantify the effect of specific space conditions on nucleic acid models. To achieve this an improved method was elaborated for the preparation of DNA and bacteriophage thin films. The homogeneity of the films was controlled by UV spectroscopy and microscopy. To provide experimental evidence for the hypothesis that interplanetary transfer of the genetic material is possible, phage T7 and isolated T7 DNA thin films have been exposed to selected space conditions: intense UVC radiation (lambda=254 nm) and high vacuum (10(-4) Pa). The effects of DNA hydration, conformation and packing on UV radiation damage were examined. Characteristic changes in the absorption spectrum, in the electrophoretic pattern of DNA and the decrease of the amount of PCR products have been detected indicating the photodamage of isolated and intraphage DNA.     

RPC-MAG The Fluxgate Magnetometer in the ROSETTA Plasma Consortium

The fluxgate magnetometer experiment onboard the ROSETTA spacecraft aims to measure the magnetic field in the interaction region of the solar wind plasma with comet 67P/Churyumov-Gerasimenko. It consists of a system of two ultra light (about 28 g each ) triaxial fluxgate magnetometer sensors, mounted on the 1.5 m long spacecraft boom. The measurement range of each sensor is ±16384 nT with quantization steps of 31 pT. The magnetometer sensors are operated with a time resolution of up to 0.05 s, corresponding to a bandwidth of 0–10 Hz. This performance of the RPC-MAG sensors allows detailed analyses of magnetic field variations in the cometary environment. RPC-MAG furthermore is designed to study possible remnant magnetic fields of the nucleus, measurements which will be done in close cooperation with the ROSETTA lander magnetometer experiment ROMAP.     

Spectroscopic Measurement of Coronal Compositions

Although the elemental composition in all parts of the solar photosphere appears to be the same this is clearly not the case with the solar upper atmosphere (SUA). Spectroscopic studies show that in the corona elemental composition along solar equatorial regions is usually different from polar regions; composition in quiet Sun regions is often different from coronal hole and active region compositions and the transition region composition is frequently different from the coronal composition along the same line of sight. In the following two issues are discussed. The first involves abundance ratios between the high-FIP O and Ne and the low-FIP Mg and Fe that are important for meaningful comparisons between photospheric and SUA compositions and the second involves a review of composition and time variability of SUA plasmas at heights of 1.0≤h≤1.5R _⊙.     

Space Shuttle UHF communications performance evaluation

Computational investigations and experimental measurements were performed to evaluate the Space Shuttle UHF communication system performance for payload bay antenna at the proposed new location. To insure adequate communication coverage at relocated new location, the link margin for the Extravehicular Activity (EVA) astronauts and between Space Shuttle Orbiter and International Space Station (SSO-ISS) during rendezvous were analyzed. The multipath effects from the vehicle structures surrounding the antenna were investigated. The Radio Frequency (RF) electromagnetic radiation to the Orbiter Docking System (ODS) pyrotechnics was also analyzed to ensure the EMC/EMI compliances.     

Road profile recognition for autonomous car navigation and Navstar GPS support

We discuss autonomous car navigation based on updating dead reckoning (DR) by road profile recognition (RPR). The navigation system requires sensors to detect changes in altitude and driving direction which are installed in modern cars for different purposes (e.g. ABS sensors). The layout of the navigation system is discussed and simulations are carried out over driving distances of approximately 150 km on the basis of realistic road data and ordinary sensor accuracies. Positioning errors of lower than 10 m (standard deviation) are observed. To achieve this accuracy the synchronization error between measured and mapped data must be continually estimated. The introduced navigation method is ideal to complete present commercial car navigation systems using Navstar GPS.     

Sc在L10-TiAl金属间化合物中占位的理论研究

基于密度泛函理论,运用第一原理赝势平面波方法,采用CASTEP量子力学能量软件包研究了Sc占据Ti位与Sc占据A l位的超胞晶格常数、原子形成热、超胞总态密度与电子密度差分图,两种情况对比下,在L10-TiA l合金中,Sc优先占据Ti位,这一结论为进一步研究三元系TiA l-Sc合金提供了理论研究的基础。     

Chaos theory and radio emission

The application of chaos theory has become popular to understand the nature of various features of solar activity because most of them are far from regular. The usual approach, however, that is based on finding low-dimensional structures of the underlying processes seems to be successful only in a few exceptional cases, such as in rather coherent phenomena as coronal pulsations. It is important to note that most phenomena in solar radio emission are more complex. We present two kinds of techniques from nonlinear dynamics which can be useful to analyse such phenomena:

1. Fragmentation processes observed in solar spike events are studied by means of symbolic dynamics methods. Different measures of complexity calculated from such observations reveal that there is some order in this fragmentation.
2. Bursts are a typical transient phenomenon. To study energization processes causing impulsive microwave bursts, the wavelet analysis is applied. It exhibits structural differences of the pre- and post-impulsive phase in cases where the power spectra of both are not distinct.

     

Biological responses to space: results of the experiment "Exobiological Unit" of ERA on EURECA I.

Spores of different strains of Bacillus subtilis and the Escherichia coli plasmid pUC19 were exposed to selected conditions of space (space vacuum and/or defined wavebands and intensities of solar ultraviolet radiation) in the experiment ER 161 "Exobiological Unit" of the Exobiology Radiation Assembly (ERA) on board of the European Retrievable Carrier (EURECA). After the approximately 11 months lasting mission, their responses were studied in terms of survival, mutagenesis in the his (B. subtilis) or lac locus (pUC19), induction of DNA strand breaks, efficiency of DNA repair systems, and the role of external protective agents. The data were compared with those of a simultaneously running ground control experiment. The survival of spores treated with the vacuum of space, however shielded against solar radiation, is substantially increased, if they are exposed in multilayers and/or in the presence of glucose as protective, whereas all spores in "artificial meteorites", i.e. embedded in clays or simulated Martian soil, are killed. Vacuum treatment leads to an increase of mutation frequency in spores, but not in plasmid DNA. Extraterrestrial solar ultraviolet radiation is mutagenic, induces strand breaks in the DNA and reduces survival substantially; however, even at the highest fluences, i.e. 3 x 10(8) J m-2, a small but significant fraction of spores survives the insolation. Action spectroscopy confirms results of previous space experiments of a synergistic action of space vacuum and solar UV radiation with DNA being the critical target.     

Experimental Aspects of Mid-Frequency Pulsations (f≈10–100 mHz) in the Southern Polar Cap

We review the results obtained in the frequency range of Pc3 (22-100 mHz) and Pc4 (7-22 mHz) pulsations at Italian Antarctic stations in the southern polar cap (“Mario Zucchelli”, at Terra Nova Bay, TNB, 80^˚.S; “Concordia”, the Italian/French base at Dome C, DMC, 89^˚.S). The absence of a midnight enhancement in the pulsation power suggests a negligible substorm influence at extreme latitudes, while the sharp noon enhancement, which appears only at TNB, is determined by the closer proximity of the station to cusp related phenomena. The relationship between the frequency of the band-limited signals and the interplanetary magnetic field strength, the cone angle influence, and the higher correlation of the Pc3 power with the solar wind speed in the morning hours suggest a global scenario in which upstream waves would be mainly responsible for the mid-frequency activity in the polar cap. However, the polarization pattern is odd with respect to the predictions for tailward propagating modes.