Spaceflight and clinorotation cause cytoskeleton and mitochondria changes and increases in apoptosis in cultured cells.

The cytoskeleton is a complex network of fibers that is sensitive to environmental factors including microgravity and altered gravitational forces. Cellular functions such as transport of cell organelles depend on cytoskeletal integrity; regulation of cytoskeletal activity plays a role in cell maintenance, cell division, and apoptosis. Here we report cytoskeletal and mitochondria alterations in cultured human lymphocyte (Jurkat) cells after exposure to spaceflight and in insect cells of Drosophila melanogaster (Schneider S-1) after exposure to conditions created by clinostat rotation. Jurkat cells were flown on the space shuttle in Biorack cassettes while Schneider S-1 cells were exposed to altered gravity forces as produced by clinostat rotation. The effects of both treatments were similar in the different cell types. Fifty percent of cells displayed effects on the microtubule network in both cell lines. Under these experimental conditions mitochondria clustering and morphological alterations of mitochondrial cristae was observed to various degrees after 4 and 48 hours of culture. Jurkat cells underwent cell divisions during exposure to spaceflight but a large number of apoptotic cells was also observed. Similar results were obtained in Schneider S-1 cells cultured under clinostat rotation. Both cell lines displayed mitochondria abnormalities and mitochondria clustering toward one side of the cells which is interpreted to be the result of microtubule disruption and failure of mitochondria transport along microtubules. The number of mitochondria was increased in cells exposed to altered gravity while cristae morphology was severely affected indicating altered mitochondria function. These results show that spaceflight as well as altered gravity produced by clinostat rotation affects microtubule and mitochondria organization and results in increases in apoptosis. Grant numbers: NAG 10-0224, NAG2-985.     

The WIND magnetic field investigation

The magnetic field experiment on WIND will provide data for studies of a broad range of scales of structures and fluctuation characteristics of the interplanetary magnetic field throughout the mission, and, where appropriate, relate them to the statics and dynamics of the magnetosphere. The basic instrument of the Magnetic Field Investigation (MFI) is a boom-mounted dual triaxial fluxgate magnetometer and associated electronics. The dual configuration provides redundancy and also permits accurate removal of the dipolar portion of the spacecraft magnetic field. The instrument provides (1) near real-time data at nominally one vector per 92 s as key parameter data for broad dissemination, (2) rapid data at 10.9 vectors s^–1 for standard analysis, and (3) occasionally, snapshot (SS) memory data and Fast Fourier Transform data (FFT), both based on 44 vectors s^–1. These measurements will be precise (0.025%), accurate, ultra-sensitive (0.008 nT/step quantization), and where the sensor noise level is <0.006 nT r.m.s. for 0–10 Hz. The digital processing unit utilizes a 12-bit microprocessor controlled analogue-to-digital converter. The instrument features a very wide dynamic range of measurement capability, from ±4 nT up to ±65 536 nT per axis in eight discrete ranges. (The upper range permits complete testing in the Earth's field.) In the FTT mode power spectral density elements are transmitted to the ground as fast as once every 23 s (high rate), and 2.7 min of SS memory time series data, triggered automatically by pre-set command, requires typically about 5.1 hours for transmission. Standard data products are expected to be the following vector field averages: 0.0227-s (detail data from SS), 0.092 s (detail in standard mode), 3 s, 1 min, and 1 hour, in both GSE and GSM coordinates, as well as the FFT spectral elements. As has been our team's tradition, high instrument reliability is obtained by the use of fully redundant systems and extremely conservative designs. We plan studies of the solar wind: (1) as a collisionless plasma laboratory, at all time scales, macro, meso and micro, but concentrating on the kinetic scale, the highest time resolution of the instrument (=0.022 s), (2) as a consequence of solar energy and mass output, (3) as an external source of plasma that can couple mass, momentum, and energy to the Earth's magnetosphere, and (4) as it is modified as a consequence of its imbedded field interacting with the moon. Since the GEOTAIL Inboard Magnetometer (GIM), which is similar to the MFI instrument, was developed by members of our team, we provide a brief discussion of GIM related science objectives, along with MFI related science goals.     

Conjecture on superrotation in planetary atmospheres: A diffusion model with mixing length theory

Superrotation on Venus is discussed in the context of comparative planetary atmospheres. In our planetary system, the rigid shell component (global average) of superrotation is ubiquitous (Jupiter, Saturn, Earth, Venus, Mars, Titan). The largest equatorial values of the component are between 25 and 150 m/sec. We present a simplified, heuristic analysis, utilizing mixing length theory to describe the small scale non-linear advections of energy and angular momentum, thereby providing a closure of the dynamic system. This leads to the conjecture that the zonal velocity may be crudely estimated by

, approximating the observed planetary trends; with c the speed of sound, the parameter a being 1 or 2 for geostrophic or cyclostrophic conditions respectively, P_α an effective Prandtl number which becomes less than one when radiative cooling is important, S_o the average stability, Γ the adiabatic lapse rate and γ the ratio of specific heats.     

Microgravity effects on sea urchin fertilization and development.

Gravity has been a pervasive influence on all living systems and there is convincing evidence to suggest that it alters fertilization and embryogenesis in several developmental systems. Notwithstanding the global importance of gravity on development, it has only been recently possible to begin to design experiments which might directly investigate the specific effects of this vector. The goal of this research program is to explore and understand the effects of gravity on fertilization and early development using sea urchins as a model system. Sea urchin development has several advantages for this project including the feasibility of maintaining and manipulating these cells during spaceflight, the high percentage of normal fertilization and early development, and the abundant knowledge about molecular, biochemical, and cellular events during embryogenesis which permits detailed insights into the mechanism by which gravity might interfere with development. Furthermore, skeletal calcium is deposited into the embryonic spicules within a day of fertilization permitting studies of the effects of gravity on bone calcium deposition.