DNA damage and repair in oncogenic transformation by heavy ion radiation.

Energetic heavy ions are present in galactic cosmic rays and solar particle events. One of the most important late effects in risk assessment is carcinogenesis. We have studied the carcinogenic effects of heavy ions at the cellular and molecular levels and have obtained quantitative data on dose-response curves and on the repair of oncogenic lesions for heavy particles with various charges and energies. Studies with repair inhibitors and restriction endonucleases indicated that for oncogenic transformation DNA is the primary target. Results from heavy ion experiments showed that the cross section increased with LET and reached a maximum value of about 0.02 micrometer2 at about 500 keV/micrometer. This limited size of cross section suggests that only a fraction of cellular genomic DNA is important in radiogenic transformation. Free radical scavengers, such as DMSO, do not give any effect on induction of oncogenic transformation by 600 MeV/u iron particles, suggesting most oncogenic damage induced by high-LET heavy ions is through direct action. Repair studies with stationary phase cells showed that the amount of reparable oncogenic lesions decreased with an increase of LET and that heavy ions with LET greater than 200 keV/micrometer produced only irreparable oncogenic damage. An enhancement effect for oncogenic transformation was observed in cells irradiated by low-dose-rate argon ions (400 MeV/u; 120 keV/micrometer). Chromosomal aberrations, such as translocation and deletion, but not sister chromatid exchange, are essential for heavy-ion-induced oncogenic transformation. The basic mechanism(s) of misrepair of DNA damage, which form oncogenic lesions, is unknown.     

Organizing and managing satellite solar power

Building an organization and management structure to create, launch, utilize and protect a satellite solar power energy system will require a global policy for the beneficial use of SSP. The fundamental organizational tasks are: (1) R&D, achieved through a project organization characterized by the integrated management of applied science, development research and construction engineering; (2) investment, generated by a series of groups creating financial vehicles for public and private investment; (3) transmission and distribution, characterized by attention to an engineering and maintenance process emphasizing high reliability; and (4) crisis response, demanding readiness for instant response to potential internal or external scenarios. A differentiated global organization spanning the long timeframe of SSP will need to have a central management core representative of all parts of the organization, with the capacity for self-renewal and re-adaptation. To be successful over its long timeframe, the SSP organization will need to build continuity and public confidence through intergenerational communication, public education, and community outreach. Integrating structures must be created at all levels of the organization, and should encompass joint work tasks and information-sharing among both industrial and government members. Developmental and alliance partners who support the formation and financing of a differentiated satellite solar power organization will share commensurately in the technologies and competencies that are created.     

Life before RNA

The hypothesis that life originated and evolved from linear informational molecules capable of facilitating their own catalytic replication is deeply entrenched. However, widespread acceptance of this paradigm seems oblivious to a lack of direct experimental support. Here, we outline the fundamental objections to the de novo appearance of linear, self-replicating polymers and examine an alternative hypothesis of template-directed coding of peptide catalysts by adsorbed purine bases. The bases (which encode biological information in modern nucleic acids) spontaneously self-organize into two-dimensional molecular solids adsorbed to the uncharged surfaces of crystalline minerals; their molecular arrangement is specified by hydrogen bonding rules between adjacent molecules and can possess the aperiodic complexity to encode putative protobiological information. The persistence of such information through self-reproduction, together with the capacity of adsorbed bases to exhibit enantiomorphism and effect amino acid discrimination, would seem to provide the necessary machinery for a primitive genetic coding mechanism.     

Preservation of martian organic and environmental records: final report of the Mars biosignature working group

The Mars Science Laboratory (MSL) has an instrument package capable of making measurements of past and present environmental conditions. The data generated may tell us if Mars is, or ever was, able to support life. However, the knowledge of Mars' past history and the geological processes most likely to preserve a record of that history remain sparse and, in some instances, ambiguous. Physical, chemical, and geological processes relevant to biosignature preservation on Earth, especially under conditions early in its history when microbial life predominated, are also imperfectly known. Here, we present the report of a working group chartered by the Co-Chairs of NASA's MSL Project Science Group, John P. Grotzinger and Michael A. Meyer, to review and evaluate potential for biosignature formation and preservation on Mars. Orbital images confirm that layered rocks achieved kilometer-scale thicknesses in some regions of ancient Mars. Clearly, interplays of sedimentation and erosional processes govern present-day exposures, and our understanding of these processes is incomplete. MSL can document and evaluate patterns of stratigraphic development as well as the sources of layered materials and their subsequent diagenesis. It can also document other potential biosignature repositories such as hydrothermal environments. These capabilities offer an unprecedented opportunity to decipher key aspects of the environmental evolution of Mars' early surface and aspects of the diagenetic processes that have operated since that time. Considering the MSL instrument payload package, we identified the following classes of biosignatures as within the MSL detection window: organism morphologies (cells, body fossils, casts), biofabrics (including microbial mats), diagnostic organic molecules, isotopic signatures, evidence of biomineralization and bioalteration, spatial patterns in chemistry, and biogenic gases. Of these, biogenic organic molecules and biogenic atmospheric gases are considered the most definitive and most readily detectable by MSL.     

The applications of lower power satellites for direct television broadcasting

The use of 12 GHz satellites for TV broadcasting directly to individual homes and small communities has been the subject of analysis and design study by groups in many countries. Implementation of the concept has been slow to follow because of the high satellite transmitter powers of from 100 to 450 W that have usually been determined to be necessary. Accumulated experience in Canada with 12 GHz operation and the evolution of technology are leading to changes in the concept of direct broadcasting such that lower power satellites may be capable of meeting the requirements.

Hermes, the Canadian/U.S. 12/14 GHz Communications Technology Satellite, has been in use for over 3 years in a program of experiments and measurements. This program has included an extensive six month experiment in direct broadcasting to 7 small communities. Experience with Hermes has shown that the signal strength is stable over long periods of time and that in Canada, significant precipitation attenuation at 12 GHz is of relatively short duration and typically occurs only during certain seasons. Operation with low propagation margins is feasible if some picture degradation and some outages at these times are acceptable. The frequency and duration of occurrence of outages can be controlled by the Earth station G/T which is cost sensitive. An individual may choose to use a low cost system with a small antenna and accept a degraded picture and outages at some times. A small community may choose to pay more for a larger antenna and lower noise receiver to achieve better performance.

Developments in technology are reducing the noise figure of mass-producible receivers from more than 6 dB to as low as 4 dB. Another technology contribution is the use of reduced bandwidth and other signal processing techniques in low-cost receivers. While use of such techniques may introduce distortions that would be unacceptable in rebroadcasting systems, there is little impact for individual and community reception. Use of both technologies reduce the required satellite EIRP or ground terminal G/T.

A field trial was begun in April 1979 to test these concepts for use in television program delivery. One hundred Earth stations capable of being tuned across a 500 MHz band and having antennas with diameters of either 1.2 m or 1.8 m are being installed for a test in Canada to receive TV signals from the 20 W transponders of ANIK-B (peak EIRP of 51 dBw) on an experimental basis. The acceptability of the video signals and the technical performance of the low-cost terminals in the bands of non-technical users are being evaluated.

The paper will summarize the concept of TV broadcasting with lower power satellites and describe the results to date of the ANIK-B field trials.     

Heliospheric and Interstellar Phenomena Deduced From Pickup ion Observations

Pickup ions, created by ionization of slow moving atoms and molecules well inside the heliosphere, provide us with a new tool to probe remote regions in and beyond the heliosphere and to study injection and acceleration processes in the solar wind. Comprehensive and continuous measurements of H, He, C, N, O, Ne and other pickup ions, especially with the Solar Wind Ion Composition Spectrometer (SWICS) on both Ulysses and ACE, have given us a wealth of data that have been used to infer chemical and physical properties of the local interstellar cloud. With SWICS on Ulysses we discovered a new population of pickup ions, produced from atomic and molecular sources deep inside the heliosphere. The velocity distributions and composition of these “inner source” pickup ions are distinctly different from those of interstellar pickup ions, showing effects of strong adiabatic cooling, and a composition resembling that of the solar wind. Strong cooling indicates that the source of these pickup ions lies close to the Sun. The similarity of composition of inner source heavy ions to that of the solar wind implies that the dominant production mechanism for these pickup ions involves the absorption and re-emission of solar wind from interplanetary dust grains. While interstellar pickup ions are the seed population of the main Anomalous Cosmic Rays (ACRs), inner source pickup ions may be an important source of the rarer ACRs such as C, Mg, Si, S, and Fe. We present new results and review previous work with an emphasis on characteristics of the local interstellar cloud and properties of the inner source. This revised version was published online in August 2006 with corrections to the Cover Date.     

Measurement of the Abundance of Helium-3 in the Sun and in the Local Interstellar Cloud with SWICS on Ulysses

The abundance of 3He in the present day local interstellar cloud (LIC) and in the sun has important implications for the study of galactic evolution and for estimating the production of light nuclei in the early universe. Data from the Solar Wind Ion Composition Spectrometer (SWICS) on Ulysses is used to measure the isotopic ratio of helium (3He/4He = ) both in the solar wind and the local interstellar cloud. For the solar wind, the unique high-latitude orbit of Ulysses allows us to study this ratio in the slow and highly dynamic wind in the ecliptic plane as well as the steady high-latitude wind of the polar coronal holes. The 3He+/4He+ ratio in the local cloud is derived from the isotopic ratio of pickup helium measured in the high-speed solar wind. In the LIC the ratio is found to be (2.48 _-0.62 ^+0.68 ) × 10^-4 with the 1- uncertainty resulting almost entirely from statistical error. In the solar wind, is determined with great statistical accuracy but shows systematic differences between fast and slow solar wind streams. The slow wind ratio is variable. Its weighted average value (4.08 ± 0.25) × 10-4 is, within uncertainties, in agreement with the Apollo SWC results. The high wind ratio is less variable but smaller. The average in the fast wind is (3.3 ± 0.3) × 10-4.     

Atomic and molecular hydrogen in interstellar space

This paper reviews the present state of knowledge of the abundances and physical state of interstellar atomic and molecular hydrogen. Much new data in this area have been obtained in recent rocket observations. There have also been new developments as a result of ground-based infrared and 21-cm observations, and theoretical research.Rocket observations of the Lyman- interstellar absorption line of atomic hydrogen indicate that, in many directions in the sky, atomic hydrogen is up to a factor of 10 less abundant than previously indicated by 21-cm emission measurements. In the direction of the Orion Nebula, most of the absorbing gas appears to be concentrated in the near vicinity of the nebula and to have a temperature considerably lower than the average of 100 K obtained from 21-cm emission measurements. Molecular hydrogen appears essentially absent from the general interstellar medium, as confirmed by theoretical studies of photodissociation processes. However, ground-based infrared and 21-cm studies indicate that the hydrogen in dark dust clouds is mostly molecular.     

Upper limit of the total magnetic flux in an active region according to the photometric-magnetic dynamical model

The photometric-magnetic dynamical model handles the evolution of an individual sunspot as an autonomous nonlinear, though integrable, dynamical system. One of its consequences is the prediction of an upper limit of the sunspot areas. This upper limit is analytically expressed by the model parameters, while its calculated value is verified by the observational data. In addition, an upper limit for the magnetic strength inside the sunspot is also predicted, and then, we obtain the following significant result: The upper limit of the total magnetic flux in an active region is found to be of about 7.23 × 10²³ Mx, namely, phenomenologically equal to the magnetic flux concentrated in the totality of the granules of the quiet Sun, having a typical maximum magnetic strength of about 12G. Therefore, the magnetic flux concentrated in an active region cannot exceed the magnetic flux concentrated in the photosphere as a whole.