Changes in vacuolation in the root apex cells of soybean seedlings in microgravity.

Changes in the vacuolation in root apex cells of soybean (Glycine max L. [Merr.]) seedlings grown in microgravity were investigated. Spaceflight and ground control seedlings were grown in the absence or presence of KMnO4 (to remove ethylene) for 6 days. After landing, in order to study of cell ultrastructure and subcellular free calcium ion distribution, seedling root apices were fixed in 2.5% (w/v) glutaraldehyde in 0.1 M cacodylate buffer and 2% (w/v) glutaraldehyde, 2.5% (w/v) formaldehyde, 2% (w/v) potassium antimonate K[Sb(OH)6] in 0.1 M K2HPO4 buffer with an osmolarity (calculated theoretically) of 0.45 and 1.26 osmol. The concentrations of ethylene in all spaceflight canisters were significantly higher than in the ground control canisters. Seedling growth was reduced in the spaceflight-exposed plants. Additionally, the spaceflight-exposed plants exhibited progressive vacuolation in the root apex cells, particularly in the columella cells, to a greater degree than the ground controls. Plasmolysis was observed in columella cells of spaceflight roots fixed in solutions with relatively high osmolarity (1.26 osmol). The appearance of plasmolysis permitted the evaluation of the water status of cells. The water potential of the spaceflight cells was higher than the surrounding fixative solution. A decrease in osmotic potential and/or an increase in turgor potential may have induced increases in cell water potential. However, the plasmolysed (i.e. non-turgid) cells implied that increases in water potential were accompanied with a decrease in osmotic potential. In such cells changes in vacuolation may have been involved to maintain turgor pressure or may have been a result of intensification of other vacuolar functions like digestion and storage.     

Mercury’s Weather-Beaten Surface: Understanding Mercury in the Context of Lunar and Asteroidal Space Weathering Studies

Mercury’s regolith, derived from the crustal bedrock, has been altered by a set of space weathering processes. Before we can interpret crustal composition, it is necessary to understand the nature of these surface alterations. The processes that space weather the surface are the same as those that form Mercury’s exosphere (micrometeoroid flux and solar wind interactions) and are moderated by the local space environment and the presence of a global magnetic field. To comprehend how space weathering acts on Mercury’s regolith, an understanding is needed of how contributing processes act as an interactive system. As no direct information (e.g., from returned samples) is available about how the system of space weathering affects Mercury’s regolith, we use as a basis for comparison the current understanding of these same processes on lunar and asteroidal regoliths as well as laboratory simulations. These comparisons suggest that Mercury’s regolith is overturned more frequently (though the characteristic surface time for a grain is unknown even relative to the lunar case), more than an order of magnitude more melt and vapor per unit time and unit area is produced by impact processes than on the Moon (creating a higher glass content via grain coatings and agglutinates), the degree of surface irradiation is comparable to or greater than that on the Moon, and photon irradiation is up to an order of magnitude greater (creating amorphous grain rims, chemically reducing the upper layers of grains to produce nanometer-scale particles of metallic iron, and depleting surface grains in volatile elements and alkali metals). The processes that chemically reduce the surface and produce nanometer-scale particles on Mercury are suggested to be more effective than similar processes on the Moon. Estimated abundances of nanometer-scale particles can account for Mercury’s dark surface relative to that of the Moon without requiring macroscopic grains of opaque minerals. The presence of nanometer-scale particles may also account for Mercury’s relatively featureless visible–near-infrared reflectance spectra. Characteristics of material returned from asteroid 25143 Itokawa demonstrate that this nanometer-scale material need not be pure iron, raising the possibility that the nanometer-scale material on Mercury may have a composition different from iron metal [such as (Fe,Mg)S]. The expected depletion of volatiles and particularly alkali metals from solar-wind interaction processes are inconsistent with the detection of sodium, potassium, and sulfur within the regolith. One plausible explanation invokes a larger fine fraction (grain size <45 μm) and more radiation-damaged grains than in the lunar surface material to create a regolith that is a more efficient reservoir for these volatiles. By this view the volatile elements detected are present not only within the grain structures, but also as adsorbates within the regolith and deposits on the surfaces of the regolith grains. The comparisons with findings from the Moon and asteroids provide a basis for predicting how compositional modifications induced by space weathering have affected Mercury’s surface composition.     

Simulation of launch and re-entry acceleration profiles for testing of shuttle and unmanned microgravity research payloads

Microgravity experiments designed for execution in Get-Away Special canisters, Hitchhiker modules, and Reusable Re-entry Satellites will be subjected to launch and re-entry accelerations. Crew-dependent provisions for preventing acceleration damage to equipment or products will not be available for these payloads during flight; therefore, the effects of launch and re-entry accelerations on all aspects of such payloads must be evaluated prior to flight. A procedure was developed for conveniently simulating the launch and re-entry acceleration profiles of the Space Shuttle (3.3 and 1.7 × g maximum, respectively) and of two versions of NASA's proposed materials research Re-usable Re-entry Satellite (8 × g maximum in one case and 4 × g in the other). By using the 7 m centrifuge of the Gravitational Plant Physiology Laboratory in Philadelphia it was found possible to simulate the time dependence of these 5 different acceleration episodes for payload masses up to 59 kg. A commercial low-cost payload device, the “Materials Dispersion Apparatus” of Instrumentation Technology Associates was tested for (1) integrity of mechanical function, (2) retention of fluid in its compartments, and (3) integrity of products under simulated re-entry g-loads. In particular, the sharp rise from 1 g to maximum g-loading that occurs during re-entry in various unmanned vehicles was successfully simulated, conditions were established for reliable functioning of the MDA, and crystals of 5 proteins suspended in compartments filled with mother liquor were subjected to this acceleration load.     

The Geology of Mercury: The View Prior to the MESSENGER Mission

Mariner 10 and Earth-based observations have revealed Mercury, the innermost of the terrestrial planetary bodies, to be an exciting laboratory for the study of Solar System geological processes. Mercury is characterized by a lunar-like surface, a global magnetic field, and an interior dominated by an iron core having a radius at least three-quarters of the radius of the planet. The 45% of the surface imaged by Mariner 10 reveals some distinctive differences from the Moon, however, with major contractional fault scarps and huge expanses of moderate-albedo Cayley-like smooth plains of uncertain origin. Our current image coverage of Mercury is comparable to that of telescopic photographs of the Earth’s Moon prior to the launch of Sputnik in 1957. We have no photographic images of one-half of the surface, the resolution of the images we do have is generally poor (∼1 km), and as with many lunar telescopic photographs, much of the available surface of Mercury is distorted by foreshortening due to viewing geometry, or poorly suited for geological analysis and impact-crater counting for age determinations because of high-Sun illumination conditions. Currently available topographic information is also very limited. Nonetheless, Mercury is a geological laboratory that represents (1) a planet where the presence of a huge iron core may be due to impact stripping of the crust and upper mantle, or alternatively, where formation of a huge core may have resulted in a residual mantle and crust of potentially unusual composition and structure; (2) a planet with an internal chemical and mechanical structure that provides new insights into planetary thermal history and the relative roles of conduction and convection in planetary heat loss; (3) a one-tectonic-plate planet where constraints on major interior processes can be deduced from the geology of the global tectonic system; (4) a planet where volcanic resurfacing may not have played a significant role in planetary history and internally generated volcanic resurfacing may have ceased at ∼3.8 Ga; (5) a planet where impact craters can be used to disentangle the fundamental roles of gravity and mean impactor velocity in determining impact crater morphology and morphometry; (6) an environment where global impact crater counts can test fundamental concepts of the distribution of impactor populations in space and time; (7) an extreme environment in which highly radar-reflective polar deposits, much more extensive than those on the Moon, can be better understood; (8) an extreme environment in which the basic processes of space weathering can be further deduced; and (9) a potential end-member in terrestrial planetary body geological evolution in which the relationships of internal and surface evolution can be clearly assessed from both a tectonic and volcanic point of view. In the half-century since the launch of Sputnik, more than 30 spacecraft have been sent to the Moon, yet only now is a second spacecraft en route to Mercury. The MESSENGER mission will address key questions about the geologic evolution of Mercury; the depth and breadth of the MESSENGER data will permit the confident reconstruction of the geological history and thermal evolution of Mercury using new imaging, topography, chemistry, mineralogy, gravity, magnetic, and environmental data.     

THE DIGITAL WAVE-PROCESSING EXPERIMENT ON CLUSTER

The wide variety of geophysical plasmas that will be investigated by the Cluster mission contain waves with a frequency range from DC to over 100 kHz with both magnetic and electric components. The characteristic duration of these waves extends from a few milliseconds to minutes and a dynamic range of over 90 dB is desired. All of these factors make it essential that the on-board control system for the Wave-Experiment Consortium (WEC) instruments be flexible so as to make effective use of the limited spacecraft resources of power and telemetry-information bandwidth. The Digital Wave Processing Experiment, (DWP), will be flown on Cluster satellites as a component of the WEC. DWP will coordinate WEC measurements as well as perform particle correlations in order to permit the direct study of wave/particle interactions. The DWP instrument employs a novel architecture based on the use of transputers with parallel processing and re-allocatable tasks to provide a high-reliability system. Members of the DWP team are also providing sophisticated electrical ground support equipment, for use during development and testing by the WEC. This is described further in Pedersen et al. (this issue).     

Rendezvous Navigation and Guidance Using a Hand-Held Self-Powered Digital Computer

This paper describes a simplified solution of the space rendezvous problem based on optical sightings taken with a hand-held sextant entered into and processed by a small digital computer. Compared to the manual method described in [1], this approach greatly reduces the astronaut's work load. Also, the computer can provide a more accurate solution, can present more flight plan options (allowing more tradeoffs between fuel usage and time), and can allow greater freedom in the sequencing of measurements and maneuvers. Physical characteristics, input-output parameters, and logic design of the computer are briefly described.     

Cytochemical localization of calcium in soybean root cap cells in microgravity.

The antimonate precipitation technique was used to evaluate the effects of microgravity and ethylene on the cellular and subcellular distribution of free calcium ions in soybean root apices. Soybean (Glycine max L. [Merr.]) dry seeds were launched, activated by hydration, and germinated in the presence of KMnO4 (to remove ethylene) and in its absence onboard the space shuttle Columbia during the STS-87 mission. Primary root apices of 6-day old seedlings were fixed for electron microscopy after landing. Ultrastructural studies indicated that antimonate precipitation appeared as individual electron-dense particles which were more or less round in shape and varied in diameter from 10 nm (minimum size beginning from which the particles were well identified) to 90 nm. It was revealed that analyzed root cap cells varied in both the precipitate particle sizes and the amount particles per unit of the cellular area. In both flight and ground control treatments, antimonate precipitation level increases from apical meristem cells to peripheral (secretory) cells of root apices. In root cap statocytes, subcellular localization of precipitate particles was revealed in the cytoplasm, nucleus and small vacuoles. The quantitative analysis showed a reduction of precipitate density in the cytoplasm and the nucleus, and an increase in precipitate density in the vacuoles from statocytes of both spaceflight treatments in comparison with ground controls.     

Topside sounding of the earth's ionosphere

The evolution of topside sounding through rocket and satellite experiments is reviewed. Topside sounding has proved valuable for revealing the structure of the high ionosphere and for investigating the resonance properties of a magnetoplasma. The limits of our present knowledge of these rapidly developing subjects are discussed. Topside sounding is predicted to have a long and useful role in exploration of the ionosphere of earth and other planets.