Osmoadaptive strategies of the archaeon Halococcus hamelinensis isolated from a hypersaline stromatolite environment

Biogenic stromatolites are sources of significant information on the evolution of microbial life. Despite their evolutionary significance, little is known about the mechanisms of osmoadaptation by microorganisms that comprise living stromatolites thriving in hypersaline environments. Osmoadaptive strategies for Halococcus hamelinensis, a novel halophilic archaeon recently isolated from living stromatolites in the hypersaline reaches of Shark Bay, were thus a particular interest in this study. To investigate the possibility of "salt-in-cytoplasm"-associated osmoadaptation for this archaeon, flame photometry studies were performed. From the results, it was evident that this halophilic archaeon did not accumulate intracellular K(+) ions when cells were exposed to either osmotic shock or conditions with gradual increments in salinity. These results were further supported by polymerase chain reaction (PCR) analyses where there was no evidence for the existence of homologous genes to an ATP-driven, high-affinity potassium uptake system in Halococcus hamelinensis. To identify an alternative salt adaptation mechanism associated with accumulation of compatible solutes for this archaeon, (1)H nuclear magnetic resonance (NMR) spectroscopy experiments were carried out. Results indicate that glycine betaine, trehalose, and glutamate are solutes likely to be involved in osmoregulation in this archeaon. Subsequent (1)H NMR analysis of cell extracts from this microorganism grown under various NaCl concentrations revealed that intracellular levels of glycine betaine increased with increasing concentrations of NaCl. This behavior of increasing glycine betaine concentration with increasing external NaCl is consistent with its identity as an osmolyte. In contrast, intracellular levels of trehalose were decreased in high concentrations of NaCl. This provides evidence that compatible solute accumulation appears to be the preferential salt regulation mechanism for this haloarchaeon, in contrast to the salt-in-cytoplasm strategy employed by many other halophilic archaea.     

Saturn kilometric radiation as a monitor for the solar wind?

Since the Voyager mission it is known that Saturn Kilometric Radiation (SKR) is strongly influenced by external forces, i.e., the solar wind and in particular the solar wind ram pressure. Recent studies using Cassini data essentially confirmed these findings for particular periods during the first Cassini orbit of Saturn. The data coverage of SKR by the Cassini/RPWS experiment for the period of six months prior to Saturn Orbit Insertion (July 1, 2004) is rather continuous, whereas there are gaps in the solar wind plasma data. The strong correlation of SKR with the solar wind may provide an indication on the variations of the solar wind plasma, specifically during the gap periods. These periods lacking solar wind data are substituted by Ulysses solar wind data which have been propagated over ∼4 AU, applying magnetohydrodynamic propagation models. Cross correlation studies showed that Ulysses solar wind data can be taken as a substitute for missing Cassini data. The use of SKR as monitor for solar wind variations is discussed. With the present set of observations the SKR proxy lacks significant reliability.     

基于双因素激励理论的高校图书馆志愿者管理机制研究

图书馆志愿者的招募、培训、考评是目前图书馆志愿者的主要管理内容。通过对现有高校图书馆志愿者管理的研究,分析其存在的不足,构建出一种新的基于双因素激励理论的图书馆志愿者管理机制,以期能更好地为用户服务。