Evidence that far-infrared radiation promotes growth of Xenopus laevis

In most ectotherms, environmental temperature has differential effects on growth and differentiation. For example, amphibian size at maturity decreases with increasing temperature. To address how radiant heat in the form of far-infrared radiation (FIR) may affect development of the aquatic ectotherm Xenopus laevis, we continuously irradiated swimming larvae as they developed into young adults. Here we report evidence that FIR promotes growth of these organisms in an aqueous environment.     

Temporal regulation of global gene expression and cellular morphology in Xenopus kidney cells in response to clinorotation.

Here, we report changes gene expression and morphology of the renal epithelial cell line, A6, which was derived from Xenopus laevis adult kidney that had been induced by long-term culturing with a three-dimensional clinostat. An oligo microarray analysis on the A6 cells showed that mRNA levels for 52 out of 8091 genes were significantly altered in response to clinorotation. On day 5, there was no dramatic change in expression level, but by day 8 and day 10, either upregulation or downregulation of gene expression became evident. By day 15, the expression levels of 18 out of 52 genes had returned to the original levels, while the remaining 34 genes maintained the altered levels of expression. Quantitative analyses of gene expression by real-time PCR confirmed that changes in the mRNA levels of selected genes were found only under clinorotation and not under hypergravity (7 g) or ground control. Morphological changes including loss of dome-like structures and disorganization of both E-cadherin adherence junctions and cortical actin were also observed after 10 days of culturing with clinorotation. These results revealed that the expression of selected genes was altered specifically in A6 cells cultured under clinorotation.     

Ground-based millimeter-wave observations of water vapor emission (183 GHz) at Atacama,Chile

We report the first results of ground-based millimeter-wave measurements of 183 GHz atmospheric water vapor spectra from Atacama highland (4800 m alt.), Chile. The measurements were carried out in December 2005 by using a spectroscopic radiometer equipped with a superconductive heterodyne receiver. A conspicuous H₂O spectrum at 183 GHz was detected with an integration time of only 1.5 min, and this is the first high frequency-resolution H₂O spectrum at 183 GHz obtained in the southern subtropical region. The vertical profile of H₂O volume mixing ratio between 40 and 64 km were retrieved from the spectrum by using the modified optimal estimation method.     

Evidence that far-infrared radiation promotes growth of Xenopus laevis.

In most ectotherms, environmental temperature has differential effects on growth and differentiation. For example, amphibian size at maturity decreases with increasing temperature. To address how radiant heat in the form of far-infrared radiation (FIR) may affect development of the aquatic ectotherm Xenopus laevis, we continuously irradiated swimming larvae as they developed into young adults. Here we report evidence that FIR promotes growth of these organisms in an aqueous environment.