Simulation experiments of the effect of space environment on bacteriophage and DNA thin films.

The main goal of PUR experiment (phage and uracil response) is to examine and quantify the effect of specific space conditions on nucleic acid models. To achieve this an improved method was elaborated for the preparation of DNA and bacteriophage thin films. The homogeneity of the films was controlled by UV spectroscopy and microscopy. To provide experimental evidence for the hypothesis that interplanetary transfer of the genetic material is possible, phage T7 and isolated T7 DNA thin films have been exposed to selected space conditions: intense UVC radiation (lambda=254 nm) and high vacuum (10(-4) Pa). The effects of DNA hydration, conformation and packing on UV radiation damage were examined. Characteristic changes in the absorption spectrum, in the electrophoretic pattern of DNA and the decrease of the amount of PCR products have been detected indicating the photodamage of isolated and intraphage DNA.     

Choroidal responses in microgravity. (SLS-1, SLS-2 and hindlimb-suspension experiments)

Fluid and electrolyte shifts occuring during human spaceflight have been reported and investigated at the level of blood, cardio-vascular and renal responses. Very few data were available concerning the cerebral fluid and electrolyte adaptation to microgravity, even in animal models. It is the reason why we developed several studies focused on the effects of spaceflight (SLS-1 and SLS-2 programs, carried on NASA STS 40 and 56 missions, which were 9- and 14-day flights, respectively), on structural and functional features of choroid plexuses, organs which secrete 70–90 % of cerebrospinal fluid (CSF) and which are involved in brain homeostasis. Rats flown aboard space shuttles were sacrificed either in space (SLS-2 experiment, on flight day 13) or 4–8 hours after landing (SLS-1 and SLS-2 experiments). Quantitative autoradiography performed by microdensitometry and image analysis, showed that lateral and third ventricle choroid plexuses from rats flown for SLS-1 experiment demonstrated an increased number (about x 2) of binding sites to natriuretic peptides (which are known to be involved in mechanisms regulating CSF production). Using electron microscopy and immunocytochemistry, we studied the cellular response of choroid plexuses, which produce cerebrospinal fluid (CSF) in brain lateral, third and fourth ventricles. We demonstrated that spaceflight (SLS-2 experiment, inflight samples) induces changes in the choroidal cell structure (apical microvilli, kinocilia organization, vesicle accumulation) and protein distribution or expression (carbonic anhydrase II, water channels,…). These observations suggested a loss of choroidal cell polarity and a decrease in CSF secretion. Hindlimb-suspended rats displayed similar choroidal changes. All together, these results support the hypothesis of a modified CSF production in rats during long-term (9, 13 or 14 days) adaptations to microgravity.     

Three-dimensional cosmic-ray simulations: Heliographic latitude and current-sheet tilt

We present the results from a study of the variations of the cosmic-ray intensity with time, heliographic latitude, and longitude, and for varying interplanetary conditions, using our three-dimensional, time-dependent computer code for cosmic-ray transport in the heliosphere. Our code also produces a solar-wind and interplanetary magnetic field (IMF) configuration which is compared with observations. Because of the fully threedimensional nature of the model calculations, we are able to model time variations which would be expected to be observed along Ulysses's trajectory as it moves to high latitudes. In particular we can model the approximately 13-and 26-day solar-rotation induced variations in cosmic rays, solar wind and IMF, as a function of increasing heliographic latitude, as one moves poleward of the interplanetary current sheet. Our preliminary model results seem to be in general form quite similar to published data, but depend on the physical parameters used such as cosmic-ray diffusion coefficients, boundary conditions, and the nature of the solar wind and IMF and current sheet.     

The Galileo Dust Detector

The Galileo Dust Detector is intended to provide direct observations of dust grains with masses between 10^-19 and 10^-9 kg in interplanetary space and in the Jovian system, to investigate their physical and dynamical properties as functions of the distances to the Sun, to Jupiter and to its satellites, to study its interaction with the Galilean satellites and the Jovian magnetosphere. Surface phenomena of the satellites (like albedo variations), which might be effects of meteoroid impacts will be compared with the dust environment. Electric charges of particulate matter in the magnetosphere and its consequences will be studied; e.g., the effects of the magnetic field on the trajectories of dust particles and fragmentation of particles due to electrostatic disruption. The investigation is performed with an instrument that measures the mass, speed, flight direction and electric charge of individual dust particles. It is a multicoincidence detector with a mass sensitivity 10⁶ times higher than that of previous in-situ experiments which measured dust in the outer solar system. The instrument weighs 4.2 kg, consumes 2.4 W, and has a normal data transmission rate of 24 bits s^-1 in nominal spacecraft tracking mode. On December 29, 1989 the instrument was switched-on. After the instrument had been configured to flight conditions cruise science data collection started immediately. In the period to May 18, 1990 at least 168 dust impacts have been recorded. For 81 of these dust grains masses and impact speeds have been determined. First flux values are given.     

Annual solar UV exposure and biological effective dose rates on the Martian surface.

The ultraviolet (UV) environment of Mars has been investigated to gain an understanding of the variation of exposure throughout a Martian year, and link this flux to biological effects and possible survival of organisms at the Martian surface. To gain an idea of how the solar UV radiation varies between different regions, including planned landing sites of two future Mars surface missions, we modelled the total solar UV surface flux throughout one Martian year for two different dust scenarios. To understand the degree of solar UV stress on micro-organisms and/or molecules essential for life on the surface of Mars, we also calculated the biologically effective dose (BED) for T7 and Uracil in relevant wavelength regions at the Martian surface as a function of season and latitude, and discuss the biological survival rates in the presence of Martian solar UV radiation. High T7/Uracil BED ratios indicate that even at high latitudes where the UV flux is significantly reduced, the radiation environment is still hostile for life due to the persisting UV-C component of the flux.     

On the levels of enzymatic substrate specificity: implications for the early evolution of metabolic pathways.

The most frequently invoked explanation for the origin of metabolic pathways is the retrograde evolution hypothesis. In contrast, according to the so-called "patchwork" theory, metabolism evolved by the recruitment of relatively inefficient small enzymes of broad specificity that could react with a wide range of chemically related substrates. In this paper it is argued that both sequence comparisons and experimental results on enzyme substrate specificity support the patchwork assembly theory. The available evidence supports previous suggestions that gene duplication events followed by a gradual neoDarwinian accumulation of mutations and other minute genetic changes lead to the narrowing and modification of enzyme function in at least some primordial metabolic pathways.     

Investigations of the Mars Upper Atmosphere with ExoMars Trace Gas Orbiter

Space Science Reviews - Time measured by an ideal clock crucially depends on the gravitational potential and velocity of the clock according to general relativity. Technological advances in...     

基于神经网络的智能刀具状态检测系统

可靠的在线刀具磨损状态检测是柔性制造系统、计算机集成制造系统以及自动化机床必不可少的一个环节。文中论述了用反传神经网络与一类模糊神经网络分析处理由力传感器和声发射传感器所测得的刀具状态信号,识别出刀具的磨损情况,从而进一步实现刀具磨损状态的在线检测,控制自动机床及时更换刀具。本研究对四种规格的钻头的磨损情况进行了全程检测,并比较分析了反传神经网络与模糊神经网络对这一问题的有效性。实验结果表明,这两种方法对处理刀具磨损状态检测均有显著的效果与很高的准确性。用一类模糊神经网络处理多传感器信息是实现刀具状态在线检测的一个极为有效的方法。     

Multimodal characteristics of a piezoelectric lead zirconate titanate element impacted with iron particles having velocities above 20 km/s

The responses of a piezoelectric lead zirconate titanate (PZT) element to hypervelocity collisions were experimentally studied. In this study, the particles of masses ranging from 0.3 to 10 fg were made to collide with PZT at velocities between 20 and 96 km/s. The amplitude and the corresponding rise time of the single-pulse output signals that were produced in the piezoelectric PZT element were measured to determine the possible collision states. The results revealed an apparently multimodal output; three classes were assumed to be involved in the pulse formation mechanism. The amplitude and rise time were sensitive to the collision velocity. The multimodal behavior implied that the PZT-based cosmic dust detectors should be calibrated according to the class they belong to.