Molecular alterations in tumorigenic human bronchial and breast epithelial cells induced by high LET radiation.

Carcinogenesis is a multi-stage process with sequence of genetic events governing the phenotypic expression of a series of transformation steps leading to the development of metastatic cancer. In the present study, immortalized human bronchial (BEP2D) and breast (MCF-10F) cells were irradiated with graded doses of either 150 keV/micrometer alpha particles or 1 GeV/nucleon 56Fe ions. Transformed cells developed through a series of successive steps before becoming tumorigenic in nude mice. Cell fusion studies indicated that radiation-induced tumorigenic phenotype in BEP2D cells could be completely suppressed by fusion with non-tumorigenic BEP2D cells. The differential expressions of known genes between tumorigenic bronchial and breast cells induced by alpha particles and their respective control cultures were compared using cDNA expression array. Among the 11 genes identified to be differentially expressed in BEP2D cells, three (DCC, DNA-PK and p21(CIP1)) were shown to be consistently down-regulated by 2 to 4 fold in all the 5 tumor cell lines examined. In contrast, their expressions in the fusion cell lines were comparable to control BEP2D cells. Similarly, expression levels of a series of genes were found to be altered in a step-wise manner among tumorigenic MCF-10F cells. The results are highly suggestive that functional alterations of these genes may be causally related to the carcinogenic process.     

典型加筋板的优化设计

对飞机结构设计中典型加筋板优化设计问题进行了研究 ,对典型加筋板形式、优化问题、计算方法和优化策略进行了描述 ,编写了优化设计程序 ,并进行了算例考核。计算结果表明 ,优化设计结果合理 ,可对该类加筋板的结构设计提供参考和指导。     

U型复合材料结构件热压罐固化变形及补偿技术应用研究

以复合材料U型构件为研究对象,采用数值模拟的方法对U型件进行热压罐温度场的模拟,模拟出在不同时间段工装和工件的温度分布情况,模拟工件在工装传热和变形影响下的内部传热、固化度、最终变形情况和残余应力水平等。根据模拟结果,优化热压罐固化工艺参数,从而改善固化热变形问题,根据分析结果制定补偿方案并实施验证,同时优化工装结构形式,采用工装型面补偿技术对零件固化变形进行预补偿。结果表明,补偿后零件变形后的外形与理论几何外形相差1.37mm,验证了补偿技术的可行性。工装型面补偿技术可以有效提高复合材料结构件的制造精度。     

Genomic instability and tumorigenic induction in immortalized human bronchial epithelial cells by heavy ions

Carcinogenesis is postulated to be a progressive multistage process characterized by an increase in genomic instability and clonal selection with each mutational event endowing a selective growth advantage. Genomic instability as manifested by the amplification of specific gene fragments is common among tumor and transformed cells. In the present study, immortalized human bronchial (BEP2D) cells were irradiated with graded doses of either 1GeV/nucleon ⁵⁶Fe ions or 150 keV/μm alpha particles. Transformed cells developed through a series of successive steps before becoming tumorigenic in nude mice. Tumorigenic cells showed neither ras mutations nor deletion in the p16 tumor suppressor gene. In contrast, they harbored mutations in the p53 gene and over-expressed cyclin D1. Genomic instability among transformed cells at various stage of the carcinogenic process was examined based on frequencies of PALA resistance. Incidence of genomic instability was highest among established tumor cell lines relative to transformed, non-tumorigenic and control cell lines. Treatment of BEP2D cells with a 4 mM dose of the aminothiol WR-1065 significantly reduced their neoplastic transforming response to ⁵⁶Fe particles. This model provides an opportunity to study the cellular and molecular mechanisms involved in malignant transformation of human epithelial cells by heavy ions.     

Rubbing-Induced Vibration Response Analysis of Dual-Rotor-Casing System

Considering gyroscopic effects caused by rotational speed,torsional vibration as well as coupling effects among inner rotor,out rotor and casing,a dynamic model of the dual-rotor-casing system is established using finite element(FE)method.By comparing the natural characteristics obtained from MATLAB and ANSYS,the developed model is verified.Then rubbing-induced vibration responses in dual-rotor-casing system are analyzed.The effects of rotational speed and speed ratio on rubbing vibration responses of the system are discussed.Results show that different combined frequency components will appear in the spectrum except two unbalanced excitation frequencies and their multiple frequency components,and these frequencies can be used as the dual-rotor aero-engine rubbing failure diagnosis frequencies when rubbing occurs.Besides,the amplitude of torsional vibration is larger than that of lateral vibration under the same working condition,and speed ratio has a great impact on the periodicity of the system rubbing-induced motion trajectory.The amplitude of rubbing-induced responses under counter-rotation is less than that under co-rotation with the same parameters.     

镍对C/SiC化学气相渗透碳的催化研究

用液相浸渗还原法将Ni渗入C/SiC复合材料,研究复合材料中Ni对化学气相渗透PyC过程的影响.用重量分析法研究Ni的催化作用,用扫描电子显微镜(SEM)、X射线衍射(XRD)技术分析材料的微观结构及组成.结果表明,Ni对化学气相渗透PyC具有催化作用,它加速了PyC的沉积,Ni含量为4.4%时增重率达最大值9.98%.沉积的PyC为细小颗粒聚集体.CVI过程中Ni与基体SiC反应生成Ni2Si,Ni3Si.     

航煤裂解气低温自点火特性与等离子体助燃研究

为获得燃料低温自点火特性以及等离子体对燃料点火的促进作用,基于快速压缩机实验系统,研究了52％He稀释、当量比1.0、压力3.4MPa、温度877.7～915.9K条件下,航煤裂解气(29.4％CH4/30.8％C2H6/21.3％C2H4/18.5％C3H6)的自点火特性,并获得其点火延迟时间.在此基础上,采用放电与...     

C/SiC复合材料渗Ni对热物理性能的影响

采用液相浸渍还原法将Ni渗入C/SiC复合材料,用SEM、XRD技术分析材料的微观结构及组成.采用热膨胀仪和激光脉冲导热仪对材料的热膨胀和热扩散性能进行研究.结果表明：Ni在复合材料内部呈颗粒聚集体态,CVD SiC涂层过程中,Ni与SiC基体反应生成Ni2Si.渗Ni后复合材料线胀系数变化趋势在200～700℃内与C/SiC原料的一致,800℃出现肩峰;再经沉积SiC涂层后线胀系数增大,但整体变化趋势仍与原料的一致.渗Ni后材料的热扩散系数明显高于C/SiC材料.Ni的渗入对C/SiC复合材料的三点弯曲强度基本无影响.     

空中交通管理系统安全绩效评估模型研究

客观准确的安全绩效评估,可以反映出空中交通管制系统运行的安全状况,并识别出安全管理存在的薄弱环节.从管制员、空中交通管制设备、环境、管理四方面找出影响系统安全运行的过程性指标,将过程性指标与结果性指标相结合构建空中交通管制系统安全绩效评估指标体系;在模糊物元分析法的基础上,对隶属度函数的确定方法进行改进,建立基于改进的模糊物元分析法的空中交通管制系统安全绩效评估模型;利用该模型对某空中交通管制单位的安全绩效进行评估,评估结果能够反映出该单位的安全状况,并指出应当改进的环节.结果表明:本模型可以对空中交通管制系统的安全绩效作出综合评判,并可以对空中交通管制系统的安全性进行量化描述.     

标准质量生产的基本原理、理念和实施对策

在介绍标准化概念、含义以及企业标准化必要性的基础上,着重介绍了标准质量生产(SQM)的基本原理、内容及其推进步骤,并探讨了SQM管理方法的理念。最后提出了对我国实施标准化管理的几项建议。