Possible applications of aquatic bioregenerative life support modules for food production in a Martian base.

Water is the essential precondition of life in general and also for the establishment of a Martian base suitable for long duration stays of humans. It is not yet proven if there is indeed a "frozen ocean" under the surface of Mars but if this could be verified it would open innovative aspects for the construction of bioregenerative life support systems (BLSS). In a general concept higher plants will play the predominant role in a Martian BLSS. It is not clear, however, how these will grow and bring seed in reduced gravity and there may be differences in the productivity in comparison to Earth conditions. Therefore, organisms which are already adapted to low gravity conditions, namely non-gravitropic aquatic plants and also aquatic animals may be used to enhance the functionality of the Martian BLSS as a whole. It has been shown already with the so-called C.E.B.A.S. MINIMODULE in the STS-89 and STS-90 spaceshuttle missions that the water plant Ceratophyllum demersum has an undisturbed and high biomass production under space conditions. Moreover, the teleost fish species Xiphophorus helleri adapted easily to the micro-g environment and maintained its normal reproductive functions. Based on this findings a possible scenario is presented in which aquatic plant production modules and combined animal-plant production systems may be used for human food production and water and air regeneration in a Martian base.     

Aquatic modules for bioregenerative life support systems based on the C.E.B.A.S. biotechnology

Most concepts for bioregenerative life support systems are based on edible higher land plants which create some problems with growth and seed generation under space conditions. Animal protein production is mostly neglected because of the tremendous waste management problems with tetrapods under reduced weightlessness. Therefore, the “Closed Equilibrated Biological Aquatic System” (C.E.B.A.S.) was developed which represents an artificial aquatic ecosystem containing aquatic organisms which are adpated at all to “near weightlessness conditions” (fishes Xiphophorus helleri, water snails Biomphalaria glabrata, ammonia oxidizing bacteria and the rootless non-gravitropic edible water plant Ceratophyllum demersum). Basically the C.E.B.A.S. consists of 4 subsystems: a ZOOLOGICASL COMPONENT (animal aquarium), a BOTANICAL COMPONENT (aquatic plant bioreactor), a MICROBIAL COMPONENT (bacteria filter) and an ELECTRONICAL COMPONENT (data acquisition and control unit). Superficially, the function principle appears simple: the plants convert light energy into chemical energy via photosynthesis thus producing biomass and oxygen. The animals and microorganisms use the oxygen for respiration and produce the carbon dioxide which is essential for plant photosynthesis. The ammonia ions excreted by the animals are converted by the bacteria to nitrite and then to nitrate ions which serve as a nitrogen source for the plants. Other essential ions derive from biological degradation of animal waste products and dead organic matter. The C.E.B.A.S. exists in 2 basic versions: the original C.E.B.A.S. with a volume of 150 liters and a self-sustaining standing time of more than 13 month and the so-called C.E.B.A.S. MINI MODULE with a volume of about 8.5 liters. In the latter there is no closed food loop by reasons of available space so that animal food has to be provided via an automated feeder. This device was flown already successfully on the STS-89 and STS-90 spaceshuttle missions and the working hypothesis was verified that aquatic organisms are nearly not affected at all by space conditions, i . e. that the plants exhibited biomass production rates identical to the ground controls and that as well the reproductive, and the immune system as the the embryonic and ontogenic development of the animals remained undisturbed. Currently the C.E.B.A.S. MINI MODLULE is prepared for a third spaceshuttle fligt (STS-107) in spring 2001. Based on the results of the space experiments a series of prototypes of aquatic food production modules for the implementation into BLSS were developed. This paper describes the scientific disposition of the STS-107 experiments and of open and closed aquaculture systems based on another aquatic plant species, the Lemnacean Wolffia arrhiza which is cultured as a vegetable in Southeastern Asia. This plant can be grown in suspension culture and several special bioreactors were developed for this purpose. W. arrhiza reproduces mainly vegetatively by buds but also sexually from time to time and is therefore especially suitable for genetic engineering, too. Therefore it was used, in addition, to optimize the C.E.B.A.S. MINI MODULE to allow experiments with a duration of 4 month in the International Space Station the basic principle of which will be explained. In the context of aquaculture systems for BLSS the continuous replacement of removed fish biomass is an essential demand. Although fish reproduction seems not to be affected in the short-term space experiments with the C.E.B.A.S. MIMI MODULE a functional and reliable hatchery for the production of siblings under reduced weightlessness is connected with some serious problems. Therefore an automated “reproduction module” for the herbivorous fish Tilapia rendalli was developed as a laboratory prototype. It is concluded that aquatic modules of different degrees of complexity can optimize the productivity of BLSS based on higher land plants and that they offer an unique opportunity for the production of animal protein in lunar or planetary bases.     

Evaluation of Turbulence Models in Predicting Hypersonic and Subsonic Base Flows Using Grid Adaptation Techniques

The flows behind the base of a generic rocket, at both hypersonic and subsonic flow conditions, are numerically studied. The main concerns are addressed to the evaluation of turbulence models and the using of grid adaptation techniques. The investiga- tion focuses on two configurations, related to hypersonic and subsonic experiments. The applicability tests of different turbulence models are conducted on the level of two-equation models calculating the steady state solution of the Reynolds-averaged Navier-Stokes(RANS) equations. All used models, the original Wilcox k-ω, the Menter shear-stress transport (SST) and the explicit algebraic Reynolds stress model(EARSM) formulation, predict an asymmetric base flow in both cases caused by the support of the models. A comparison with preliminary experimental results indicates a preference for the SST and EARSM results over the results from the older k-ω model. Sensitivity studies show no significant influence of the grid topology or the location of the laminar to turbulent transition on the base flow field, but a strong influence of even small angles of attack is reported from the related experiments.     

Solar Weather Event Modelling and Prediction

Key drivers of solar weather and mid-term solar weather are reviewed by considering a selection of relevant physics- and statistics-based scientific models as well as a selection of related prediction models, in order to provide an updated operational scenario for space weather applications. The characteristics and outcomes of the considered scientific and prediction models indicate that they only partially cope with the complex nature of solar activity for the lack of a detailed knowledge of the underlying physics. This is indicated by the fact that, on one hand, scientific models based on chaos theory and non-linear dynamics reproduce better the observed features, and, on the other hand, that prediction models based on statistics and artificial neural networks perform better. To date, the solar weather prediction success at most time and spatial scales is far from being satisfactory, but the forthcoming ground- and space-based high-resolution observations can add fundamental tiles to the modelling and predicting frameworks as well as the application of advanced mathematical approaches in the analysis of diachronic solar observations, that are a must to provide comprehensive and homogeneous data sets.     

Determination of temperature maps of EUV coronal hole jets

Coronal hole jets are fast ejections of plasma occurring within coronal holes, observed at Extreme-UltraViolet (EUV) and X-ray wavelengths. Recent observations of jets by the STEREO and Hinode missions show that they are transient phenomena which occur at much higher rates than large-scale impulsive phenomena like flares and Coronal Mass Ejections (CMEs). In this paper we describe some typical characteristics of coronal jets observed by the SECCHI instruments of STEREO spacecraft. We show an example of 3D reconstruction of the helical structure for a south pole jet, and present how the angular distribution of the jet position angles changes from the Extreme-UltraViolet-Imager (EUVI) field of view to the CORonagraph1 (COR1) (height ∼2.0 R_⊙ heliocentric distance) field of view. Then we discuss a preliminary temperature determination for the jet plasma by using the filter ratio method at 171 and 195 Å and applying a technique for subtracting the EUV background radiation. The results show that jets are characterized by electron temperatures ranging between 0.8 and 1.3 MK. We present the thermal structure of the jet as temperature maps and we describe its thermal evolution.     

On the practical exploitation of perturbative effects in low Earth orbit for space debris mitigation

This paper presents the results of a numerical evaluation of the natural lifetime reduction in low Earth orbit, due to dynamical perturbations. The study considers two values for the area-to-mass ratio, a nominal ratio which resembles a typical value of spacecraft in orbit today, and an enhanced ratio which covers the surface augmentation. The results were obtained with two orbit propagators, one of a semi-analytical nature and the second one using non-averaged equations of motion. The simulations for both propagators were set up similarly to allow comparison. They both use the solar radiation pressure and the secular terms of the geopotential ($J_2,J_4$ and $J_6$). The atmospheric drag was turned on and off in both propagators to alternatively study the eccentricity build up and the residual lifetime. The non-averaging case also covers a validation with the full 6?×?6 geopotential. The results confirm the findings in previous publications, that is, the possibility for de-orbiting from altitudes above the residual atmosphere if a solar sail is deployed at the end-of-life, due to the combined effect of solar radiation pressure and the oblateness of the Earth. At near polar inclinations, shadowing effects can be exploited to the same end. The results obtained with the full, non-averaging propagator revealed additional de-orbiting corridors associated with solar radiation pressure which were not found by previous work on space debris mitigation. The results of both tools are compared for specific initial conditions. For nominal values of area-to-mass ratio, instead, it is confirmed that this resonance effect is negligible.The paper then puts the findings in the perspective of the current satellite catalogue. It identifies space missions which are currently close to a resonance corridor and shows the orbit evolution within the resonances with a significantly shorter residual orbital lifetime. The paper finishes with a discussion on the exploitation of these effects with regards to the long-term simulation of the space debris environment and a flux and collision probability comparison.     

Aquatic modules for bioregenerative life support systems based on the C.E.B.A.S. biotechnology [correction of biotechnilogy

Most concepts for bioregenerative life support systems are based on edible higher land plants which create some problems with growth and seed generation under space conditions. Animal protein production is mostly neglected because of the tremendous waste management problems with tetrapods under reduced weightlessness. Therefore, the "Closed Equilibrated Biological Aquatic System" (C.E.B.A.S.) was developed which represents an artificial aquatic ecosystem containing aquatic organisms which are adapted at all to "near weightlessness conditions" (fishes Xiphophorus helleri, water snails Biomphalaria glabrata, ammonia oxidizing bacteria and the rootless non-gravitropic edible water plant Ceratophyllum demersum). Basically the C.E.B.A.S. consists of 4 subsystems: a ZOOLOGICAL (correction of ZOOLOGICASL) COMPONENT (animal aquarium), a BOTANICAL COMPONENT (aquatic plant bioreactor), a MICROBIAL COMPONENT (bacteria filter) and an ELECTRONICAL COMPONENT (data acquisition and control unit). Superficially, the function principle appears simple: the plants convert light energy into chemical energy via photosynthesis thus producing biomass and oxygen. The animals and microorganisms use the oxygen for respiration and produce the carbon dioxide which is essential for plant photosynthesis. The ammonia ions excreted by the animals are converted by the bacteria to nitrite and then to nitrate ions which serve as a nitrogen source for the plants. Other essential ions derive from biological degradation of animal waste products and dead organic matter. The C.E.B.A.S. exists in 2 basic versions: the original C.E.B.A.S. with a volume of 150 liters and a self-sustaining standing time of more than 13 month and the so-called C.E.B.A.S. MINI MODULE with a volume of about 8.5 liters. In the latter there is no closed food loop by reasons of available space so that animal food has to be provided via an automated feeder. This device was flown already successfully on the STS-89 and STS-90 spaceshuttle missions and the working hypothesis was verified that aquatic organisms are nearly not affected at all by space conditions, i.e. that the plants exhibited biomass production rates identical to the sound controls and that as well the reproductive, and the immune system as the embryonic and ontogenic development of the animals remained undisturbed. Currently the C.E.B.A.S. MINI MODLULE is prepared for a third spaceshuttle flight (STS-107) in spring 2001. Based on the results of the space experiments a series of prototypes of aquatic food production modules for the implementation into BLSS were developed. This paper describes the scientific disposition of the STS-107 experiment and of open and closed aquaculture systems based on another aquatic plant species, the Lemnacean Wolffia arrhiza which is cultured as a vegetable in Southeastern Asia. This plant can be grown in suspension culture and several special bioreactors were developed for this purpose. W. arrhiza reproduces mainly vegetatively by buds but also sexually from time to time and is therefore especially suitable for genetic engineering, too. Therefore it was used, in addition, to optimize the C.E.B.A.S. MINI MODULE to allow experiments with a duration of 4 month in the International Space Station the basic principle of which will be explained. In the context of aquaculture systems for BLSS the continuous replacement of removed fish biomass is an essential demand. Although fish reproduction seems not to be affected in the shortterm space experiments with the C.E.B.A.S. MINI MODULE a functional and reliable hatchery for the production of siblings under reduced weightlessness is connected with some serious problems. Therefore an automated "reproduction module" for the herbivorous fish Tilapia rendalli was developed as a laboratory prototype. It is concluded that aquatic modules of different degrees of complexity can optimize the productivity of BLSS based on higher land plants and that they offer an unique opportunity for the production of animal protein in lunar or planetary bases.     

The C.E.B.A.S.-Minimodule: behaviour of an artificial aquatic ecological system during spaceflight.

The C.E.B.A.S.-Minimodule, a closed aquatic ecosystem integrated into a middeck locker and consisting of a Zoological (animal tanks), a Botanical (plant bioreactor), a Microbial (bacteria filter) and an Electronic Component (data acquisition/control system) was flown on the STS-89 spaceshuttle mission in January 1998 for 9 days. Preflight the plant bioreactor was loaded with 53 g of Ceratophyllum demersum (coontail) and the animal tanks with 4 adult pregnant females of the fish, Xiphophorus helleri (sword-tails), 200 juveniles of the same species less than 1 week of age, 38 large and 30 juvenile Biomphalaria glabrata water snails. The filter compartment was filled with 200 g of lava grain inoculated with laboratory strains of ammonia-oxidizing bacteria. A ground reference was undertaken with the same biological setup with a delay of 4 d. After an adaptation period of 5 d the system was closed and integrated into the spaceshuttle one day before launch. Video recordings of the animals were automatically taken for 10 minutes in 2-hour periods; the tapes were changed daily by the astronauts. The chemical and physical data for the aquatic system were within the expected range and were closely comparable in comparison to the ground reference. After 9 d under space conditions, the plant biomass increased to 117 g. The plants were all found in very good condition. All 4 adult female fish were retrieved in a good physiological condition. The juvenile fishes had a survival rate of about 33%. Almost 97% of the snails had survived and produced more than 250 neonates and 40 spawning packs. All samples were distributed according to a defined schedule and satisfied all scientific needs of the involved 12 principal investigators. This was the first successful spaceflight of an artificial aquatic ecosystem containing vertebrates, invertebrates, higher plants and microorganisms self-sustained by its inhabitants only. C.E.B.A.S. in a modified form and biological setup is a promising candidate for the early space station utilization as a first midterm experiment.