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The recent discovery of methane in the martian atmosphere is arguably one of the most important discoveries in the field of astrobiology. One possible source of this methane could be a microorganism analogous to those on Earth in the domain Archaea known as methanogens. Methanogens are described as obligately anaerobic, and methods developed to work with methanogens typically include anaerobic media and buffers, gassing manifolds, and possibly anaerobic chambers. To determine if the time, effort, and supplies required to maintain anaerobic conditions are necessary to maintain viability, we compared anaerobically washed cells with cells that were washed in the presence of atmospheric oxygen. Anaerobic tubes were opened, and cultures were poured into plastic centrifuge tubes, centrifuged, and suspended in fresh buffer, all in the presence of atmospheric oxygen. Washed cells from both aerobic and anaerobic procedures were inoculated into methanogenic growth media under anaerobic conditions and incubated at temperatures conducive to growth for each methanogenic strain tested. Methane production was measured at time intervals using a gas chromatograph. In three strains, significant differences were not seen between aerobically and anaerobically washed cells. In one strain, there was significantly less methane production observed following aerobic washing at some time points; however, substantial methane production occurred following both procedures. Thus, it appears that aerobic manipulations for relatively short periods of time with at least a few species of methanogens may not lead to loss of viability. With the discovery of methane in the martian atmosphere, it is likely that there will be an increase in astrobiology-related methanogen research. The research reported here should simplify the methodology. 相似文献
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瑞士威力铭-马科黛尔公司 《航空制造技术》2008,(13)
瑞士加工中心制造商威力铭-马科黛尔公司着重于质量和生产效率,主要活跃于航空/航天、精密机械、钟表和医疗行业,并以其为用户提供的高性能交钥匙解决方案而著称. 相似文献
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采用基于密度泛函理论(DFT)的第一性原理计算方法,研究了M2CoA型Heusler合金Mn2CoAl、Mn2CoSi、Ti2CoAl和Ti2CoSi的电子结构和磁学性质。发现Heusler合金Mn2CoAl是亚铁磁性自旋无带隙半导体,Mn2CoSi与Ti2CoAl是亚铁磁性自旋半金属,而Ti2CoSi是铁磁性自旋半金属。它们的总自旋磁矩均为整数,符合Slater-Pauling规则。然后,在分析电子能带结构和态密度的基础上,探讨了自旋无带隙半导体与半金属性的根源。最后,声子谱和弹性常数计算结果表明所有M2CoA型Heusler合金在晶格动力学和力学上均是稳定的。 相似文献
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