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The recent discovery of methane in the martian atmosphere is arguably one of the most important discoveries in the field of astrobiology. One possible source of this methane could be a microorganism analogous to those on Earth in the domain Archaea known as methanogens. Methanogens are described as obligately anaerobic, and methods developed to work with methanogens typically include anaerobic media and buffers, gassing manifolds, and possibly anaerobic chambers. To determine if the time, effort, and supplies required to maintain anaerobic conditions are necessary to maintain viability, we compared anaerobically washed cells with cells that were washed in the presence of atmospheric oxygen. Anaerobic tubes were opened, and cultures were poured into plastic centrifuge tubes, centrifuged, and suspended in fresh buffer, all in the presence of atmospheric oxygen. Washed cells from both aerobic and anaerobic procedures were inoculated into methanogenic growth media under anaerobic conditions and incubated at temperatures conducive to growth for each methanogenic strain tested. Methane production was measured at time intervals using a gas chromatograph. In three strains, significant differences were not seen between aerobically and anaerobically washed cells. In one strain, there was significantly less methane production observed following aerobic washing at some time points; however, substantial methane production occurred following both procedures. Thus, it appears that aerobic manipulations for relatively short periods of time with at least a few species of methanogens may not lead to loss of viability. With the discovery of methane in the martian atmosphere, it is likely that there will be an increase in astrobiology-related methanogen research. The research reported here should simplify the methodology. 相似文献
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The jets of icy particles and water vapor issuing from the south pole of Enceladus are evidence for activity driven by some geophysical energy source. The vapor has also been shown to contain simple organic compounds, and the south polar terrain is bathed in excess heat coming from below. The source of the ice and vapor, and the mechanisms that accelerate the material into space, remain obscure. However, it is possible that a liquid water environment exists beneath the south polar cap, which may be conducive to life. Several theories for the origin of life on Earth would apply to Enceladus. These are (1) origin in an organic-rich mixture, (2) origin in the redox gradient of a submarine vent, and (3) panspermia. There are three microbial ecosystems on Earth that do not rely on sunlight, oxygen, or organics produced at the surface and, thus, provide analogues for possible ecologies on Enceladus. Two of these ecosystems are found deep in volcanic rock, and the primary productivity is based on the consumption by methanogens of hydrogen produced by rock reactions with water. The third ecosystem is found deep below the surface in South Africa and is based on sulfur-reducing bacteria consuming hydrogen and sulfate, both of which are ultimately produced by radioactive decay. Methane has been detected in the plume of Enceladus and may be biological in origin. An indicator of biological origin may be the ratio of non-methane hydrocarbons to methane, which is very low (0.001) for biological sources but is higher (0.1-0.01) for nonbiological sources. Thus, Cassini's instruments may detect plausible evidence for life by analysis of hydrocarbons in the plume during close encounters. 相似文献
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The relatively recent discoveries that liquid water likely existed on the surface of past Mars and that methane currently exists in the martian atmosphere have fueled the possibility of extant or extinct life on Mars. One possible explanation for the existence of the methane would be the presence of methanogens in the subsurface. Methanogens are microorganisms in the domain Archaea that can metabolize molecular hydrogen as an energy source and carbon dioxide as a carbon source and produce methane. One factor of importance is the arid nature of Mars, at least at the surface. If one is to assume that life exists below the surface, then based on the only example of life that we know, liquid water must be present. Realistically, however, that liquid water may be seasonal just as it is at some locations on our home planet. Here we report on research designed to determine how long certain species of methanogens can survive desiccation on a Mars soil simulant, JSC Mars-1. Methanogenic cells were grown on JSC Mars-1, transferred to a desiccator within a Coy anaerobic environmental chamber, and maintained there for varying time periods. Following removal from the desiccator and rehydration, gas chromatographic measurements of methane indicated survival for varying time periods. Methanosarcina barkeri survived desiccation for 10 days, while Methanobacterium formicicum and Methanothermobacter wolfeii were able to survive for 25 days. 相似文献
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Recent experience with different turbulence models applied to the calculation of flow over aircraft components 总被引:5,自引:0,他引:5
An evaluation of ten turbulence models is made for compressible flows encountered in current aircraft applications. The Baldwin–Lomax and P.D. Thomas algebraic models, the Baldwin–Barth and Spalart–Allmaras one-equation models, five low-Reynolds-number k– models and the Menter SST blended k–/k–ω model are examined. A zonal, upwind, implicit, factored algorithm is used to solve both the mean flow equations and the turbulence model equations for three-dimensional, compressible turbulent flow. Calculations are presented for both internal and external flowfields including a two-stream mixing layer, a supersonic flat-plate boundary layer, a transonic supercritical airfoil, a shock wave/turbulent boundary layer interaction, an ejector nozzle, a highly offset diffuser, and a twin impinging jet flowfield. The influence of two modifications to the production of turbulent kinetic energy for the low-Reynolds-number k– models is evaluated, a vorticity-based strain rate and a production limiter. A compressibility correction for high speed shear layers is also examined. Comparisons of the results of the various turbulence models are made with experimental measurements. Significant differences are observed in the model predictions when applied to the same problem using the same computational mesh and mean flow solver. The algebraic models are unable to capture the physics of these complex flowfields, particularly for the internal flow calculations. The performance of each model is dependent on the application. No universal model is found for all flowfields examined. Each one-equation and two-equation model has specific strengths and weaknesses and the performance of each model is assessed. 相似文献
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用于铝合金的集成计算材料工程是将微观(10-10~10-8m)、细观(10-8~10-4m)、介观(10-4~10-2m)和宏观(10-2~10 m)等多尺度计算模拟和关键实验集成到铝合金设计开发的全过程中,通过成分-工艺-结构-性能的集成化,把铝合金的研发由传统经验式提升到以组织演化及其与性能相关性为基础的科学设计上,从而大大加快其研发速度,降低研发成本。本文详细阐述了原子尺度模拟、相图计算、相场、元胞自动机和有限元等计算模拟方法及微结构表征和性能测定的实验方法,论述了其在铝合金研发中所发挥的具体作用。基于集成计算材料工程,提出了从用户需要、设计制备和工业生产3个层面研发铝合金的具体框架。通过2个应用实例,展示了集成计算材料工程在铝合金研发中的强大功能,这也为新型铝合金及其它新材料的设计和开发提供了新模式。 相似文献
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Methanogens have not been shown to metabolize in conditions exactly analogous to those present in Mars' subsurface. In typical studies of methanogenic metabolism, nutrient-rich buffered media and reducing agents are added to the cultures in an attempt to optimize the environment for methanogen survival and growth. To study methanogens in more Mars-relevant laboratory conditions, efforts should be made to eliminate artificial media, buffers, and reducing agents from investigations of methanogenic metabolism. After preliminary work to compare methanogen viability on montmorillonite clay and JSC Mars-1 regolith simulant, a study was conducted to determine whether biological methanogenesis could occur in non-reduced, non-buffered environments containing only H(2), CO(2), montmorillonite, and the liquid fraction extracted from a montmorillonite/deionized water suspension. Biogenic methane was observed in the microenvironments despite the omission of traditional media, buffers, and reducing agents. Mean headspace methane concentration after 96 days of observation was 10.23%?±?0.64% (% vol?±?SEM, n?=?4). However, methane production was severely decreased with respect to reduced, buffered microenvironments (Day 28: 31.98%?±?0.19%, n?=?3). Analysis of results and comparison to previous work indicate that montmorillonite clay has a strong ability to supply micronutrients necessary for methanogenic metabolism, and the liquid fraction from a montmorillonite/deionized water slurry can successfully be used as an alternative to reduced and buffered nutritive media in Mars-relevant studies of methanogenic metabolism. 相似文献
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为了研究水润滑高分子材料轴承的微观界面的流场特性,本文以单个微凹体中的流场作为研究对象,建立了单个微凹体流动模型。采用多松弛格子玻尔兹曼方法MRT-LBM (multi-relaxation time lattice Boltzmann method)对单个微凹体的流体流动问题进行数值模拟。研究了流场中速度的变化规律,以及流场中流线分布随雷诺数的变化规律。结果表明当雷诺数较低时,流场处于稳定性流动状态;当雷诺数达到一定程度时,流场处于周期流动状态;当雷诺数很高时,流场处于湍流运动状态。探究微观流场中复杂的变化规律,为研究水润滑高分子材料轴承的润滑和摩擦性能提供一些理论支撑。 相似文献
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针对车载捷联惯导系统(SINS),提出一种快速行进间粗对准方法。将捷联矩阵分解为3个矩阵相乘,利用GPS提供的载体在导航坐标系的速度和里程计提供的载体在载体坐标系的速度构建不共线向量,求解初始载体坐标系相对惯性坐标系的常值转换矩阵,进而求得初始姿态矩阵。该对准方法对载车的唯一要求是在对准过程中做一个转弯机动。与现有对准方法相比,该方法没有用到加速度计信息。仿真结果表明,该方法能在1 min之内完成粗对准,采用零偏为0.1(°)/h的低精度陀螺,对准误差小于0.3°。 相似文献
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