Preservation of martian organic and environmental records: final report of the Mars biosignature working group

The Mars Science Laboratory (MSL) has an instrument package capable of making measurements of past and present environmental conditions. The data generated may tell us if Mars is, or ever was, able to support life. However, the knowledge of Mars' past history and the geological processes most likely to preserve a record of that history remain sparse and, in some instances, ambiguous. Physical, chemical, and geological processes relevant to biosignature preservation on Earth, especially under conditions early in its history when microbial life predominated, are also imperfectly known. Here, we present the report of a working group chartered by the Co-Chairs of NASA's MSL Project Science Group, John P. Grotzinger and Michael A. Meyer, to review and evaluate potential for biosignature formation and preservation on Mars. Orbital images confirm that layered rocks achieved kilometer-scale thicknesses in some regions of ancient Mars. Clearly, interplays of sedimentation and erosional processes govern present-day exposures, and our understanding of these processes is incomplete. MSL can document and evaluate patterns of stratigraphic development as well as the sources of layered materials and their subsequent diagenesis. It can also document other potential biosignature repositories such as hydrothermal environments. These capabilities offer an unprecedented opportunity to decipher key aspects of the environmental evolution of Mars' early surface and aspects of the diagenetic processes that have operated since that time. Considering the MSL instrument payload package, we identified the following classes of biosignatures as within the MSL detection window: organism morphologies (cells, body fossils, casts), biofabrics (including microbial mats), diagnostic organic molecules, isotopic signatures, evidence of biomineralization and bioalteration, spatial patterns in chemistry, and biogenic gases. Of these, biogenic organic molecules and biogenic atmospheric gases are considered the most definitive and most readily detectable by MSL.     

Molecular diversity of cyanobacteria inhabiting coniform structures and surrounding mat in a Yellowstone hot spring

Lithified coniform structures are common within cyanobacterial mats in Yellowstone National Park hot springs. It is unknown whether these structures and the mats from which they develop are inhabited by the same cyanobacterial populations. Denaturing gradient gel electrophoresis and sequencing and phylogenetic analysis of 16S rDNA was used to determine whether (1) three different morphological types of lithified coniform structures are inhabited by different cyanobacterial species, (2) these species are partitioned along a vertical gradient of these structures, and (3) lithified and non-lithified sections of mat are inhabited by different cyanobacterial species. Our results, based on multiple samplings, indicate that the cyanobacterial community compositions in the three lithified morphological types were identical and lacked any vertical differentiation. However, lithified and non-lithified portions of the same mat were inhabited by distinct and different populations of cyanobacteria. Cyanobacteria inhabiting lithified structures included at least one undefined Oscillatorialean taxon, which may represent the dominant cyanobacteria genus in lithified coniform stromatolites, Phormidium, three Synechococcus-like species, and two unknown cyanobacterial taxa. In contrast, the surrounding mats contained four closely related Synechococcus-like species. Our results indicate that the distribution of lithified coniform stromatolites may be dependent on the presence of one or more microorganisms, which are phylogenetically different from those inhabiting surrounding non-lithified mats.     

XANES analysis of organic residues produced from the UV irradiation of astrophysical ice analogs

Organic residues formed in the laboratory from the ultraviolet (UV) photo-irradiation or ion bombardment of astrophysical ice analogs have been extensively studied for the last 15 years with a broad suite of techniques, including infrared (IR) and UV spectroscopies, as well as mass spectrometry. Analyses of these materials show that they consist of complex mixtures of organic compounds stable at room temperature, mostly soluble, that have not been fully characterized. However, the hydrolysis products of these residues have been partly identified using chromatography techniques, which indicate that they contain molecular precursors of prebiotic interest such as amino acids, nitrile-bearing compounds, and amphiphilic compounds. In this study, we present the first X-ray absorption near-edge structure (XANES) spectroscopy measurements of three organic residues made from the UV irradiation of ices having different starting compositions. XANES spectra confirm the presence of different chemical functions in these residues, and indicate that they are rich in nitrogen- and oxygen-bearing species. These data can be compared with XANES measurements of extraterrestrial materials. Finally, this study also shows how soft X rays can alter the chemical composition of samples.     

Phobos regolith simulants PGI-1 and PCA-1

The Martian moon Phobos is the target of the upcoming JAXA Martian Moons eXploration (MMX) mission. There are currently no known samples of Phobos, so spacecraft hardware testing and scientific studies require Phobos regolith simulants. Here, we present two new Phobos regolith simulants: Phobos Captured Asteroid-1 (PCA-1) and Phobos Giant Impact (PGI-1). These two simulants reflect the two hypotheses for Phobos’s formation, and thus the two broad possibilities for composition. This work follows previous efforts to document the development of mineralogically accurate simulants, in an effort to overcome past pitfalls with inappropriate uses of simulants. We report physical and geotechnical properties of PCA-1 and PGI-1, including their reflectance spectra, grain size distributions, abrasivity, cohesion, strength, and hardness. Our intent is for PCA-1 and PGI-1 to be open standards for Phobos simulants, and we present the simulant recipes and production methodology for use and modification by the community.     

Lagrangian coherent structures in various map representations for application to multi-body gravitational regimes

The Finite-Time Lyapunov Exponent (FTLE) has been demonstrated as an effective metric for revealing distinct, bounded regions within a flow. The dynamical differential equations derived in multi-body gravitational environments model a flow that governs the motion of a spacecraft. Specific features emerge in an FTLE map, denoted Lagrangian Coherent Structures (LCS), that define the extent of regions that bound qualitatively different types of behavior. Consequently, LCS supply effective barriers to transport in a generic system, similar to the notion of invariant manifolds in autonomous systems. Unlike traditional invariant manifolds associated with solutions in an autonomous system, LCS evolve with the flow in time-dependent systems while continuing to bound distinct regions of behavior. Moreover, in general, FTLE values supply information describing the relative sensitivity in the neighborhood of a trajectory. Here, different models and variable representations are used to generate maps of FTLE, and the resulting structures are applied to design and analysis within an astrodynamical context. Application of FTLE and LCS to transfers from LEO to the L₁ region in the Earth–Moon system are presented and discussed. In an additional example, an FTLE analysis is offered of a few stationkeeping maneuvers from the Earth–Moon mission ARTEMIS (Acceleration, Reconnection, Turbulence and Electrodynamics of the Moon's Interaction with the Sun).