The role of DNA repair on cell killing by charged particles.

It can be noted that it is not simple double strand breaks (dsb) but the non-reparable breaks that are associated with high biological effectiveness in the cell killing effect for high LET radiation. Here, we have examined the effectiveness of fast neutrons and low (initial energy = 12 MeV/u) or high (135 MeV/u) energy charged particles on cell death in 19 mammalian cell lines including radiosensitive mutants. Some of the radiosensitive lines were deficient in DNA dsb repair such as LX830, M10, V3, and L5178Y-S cells and showed lower values of relative biological effectiveness (RBE) for fast neutrons if compared with their parent cell lines. The other lines of human ataxia-telangiectasia fibroblasts, irs 1, irs 2, irs 3 and irs1SF cells, which were also radiosensitive but known as proficient in dsb repair, showed moderated RBEs. Dsb repair deficient mutants showed low RBE values for heavy ions. These experimental findings suggest that the DNA repair system does not play a major role against the attack of high linear energy transfer (LET) radiations. Therefore, we hypothesize that a main cause of cell death induced by high LET radiations is due to non-reparable dsb, which are produced at a higher rate compared to low LET radiations.     

Austrian dose measurements onboard space station MIR and the International Space Station--overview and comparison.

The Atominstitute of the Austrian Universities has conducted various space research missions in the last 12 years in cooperation with the Institute for Biomedical Problems in Moscow. They dealt with the exact determination of the radiation hazards for cosmonauts and the development of precise measurement devices. Special emphasis will be laid on the last experiment on space station MIR the goal of which was the determination of the depth distribution of absorbed dose and dose equivalent in a water filled Phantom. The first results from dose measurements onboard the International Space Station (ISS) will also be discussed. The spherical Phantom with a diameter of 35 cm was developed at the Institute for Biomedical Problems and had 4 channels where dosimeters can be exposed in different depths. The exposure period covered the timeframe from May 1997 to February 1999. Thermoluminescent dosimeters (TLDs) were exposed inside the Phantom, either parallel or perpendicular to the hull of the spacecraft. For the evaluation of the linear energy transfer (LET), the high temperature ratio (HTR) method was applied. Based on this method a mean quality factor and, subsequently, the dose equivalent is calculated according to the Q(LET infinity) relationship proposed in ICRP 26. An increased contribution of neutrons could be detected inside the Phantom. However the total dose equivalent did not increase over the depth of the Phantom. As the first Austrian measurements on the ISS dosimeter packages were exposed for 248 days, starting in February 2001 at six different locations onboard the ISS. The Austrian dosimeter sets for this first exposure on the ISS contained five different kinds of passive thermoluminescent dosimeters. First results showed a position dependent absorbed dose rate at the ISS.     

Carbon dioxide and oxygen budgets of a plant cultural system in a CELSS--a case of cultivation of lettuce and turnips.

In order to collect basic data about CO2 and O2 budgets of a plant cultural system in a CELSS, the variation of the CO2 absorption rates of lettuce and turnips were observed during the growing period, under different conditions. The O2 release rates were deduced from the CO2 absorption rates multiplied by 32/44. As a result, when the light intensity, the photoperiod and the atmospheric CO2 concentration increased, the rates also increased. The effects on the turnips were more significant than those on the lettuce. Turnips at 310 micromoles/m2/s of PPFD, 24 hours of photoperiod and 1100 ppm of CO2 concentration grew most actively in the present experimental conditions. One turnip absorbed 32.3 g CO2 and released 23.5 g O2 for 6 days between 24 days and 30 days after sowing.     

The influence of dose, dose-rate and particle fragmentation on cataract induction by energetic iron ions.

Because activities in space necessarily involve chronic exposure to a heterogeneous charged particle radiation field it is important to assess the influence of dose-rate and the possible modulating role of heavy particle fragmentation on biological systems. Using the well-studied cataract model, mice were exposed to plateau 600 MeV/amu 56Fe ions either as acute or fractionated exposures at total doses of 5 - 504 cGy. Additional groups of mice received 20, 360 and 504 cGy behind 50 mm of polyethylene, which simulates body shielding. The reference radiation consisted of 60Co gamma radiation. The animals were examined by slit lamp biomicroscopy over their three year life spans. In accordance with our previous observations with heavy particles, the cataractogenic potential of the 600 MeV/amu 56Fe ions was greater than for low-LET radiation and increased with decreasing dose relative to gamma-rays. Fractionation of a given dose of 56Fe ions did not reduce the cataractogenicity of the radiation compared to the acute regimen. Fragmentation of the beam in the polyethylene did not alter the cataractotoxicity of the ions, either when administered singly or in fractions.     

Space Shuttle UHF communications performance evaluation

Computational investigations and experimental measurements were performed to evaluate the Space Shuttle UHF communication system performance for payload bay antenna at the proposed new location. To insure adequate communication coverage at relocated new location, the link margin for the Extravehicular Activity (EVA) astronauts and between Space Shuttle Orbiter and International Space Station (SSO-ISS) during rendezvous were analyzed. The multipath effects from the vehicle structures surrounding the antenna were investigated. The Radio Frequency (RF) electromagnetic radiation to the Orbiter Docking System (ODS) pyrotechnics was also analyzed to ensure the EMC/EMI compliances.     

Space experiment on behaviors of treefrog.

Japanese treefrogs (Hyla japonica) are planned to be sent to the space station MIR. Experimental system was developed to observe their behaviors under microgravity.     

Distance estimation at 60 GHz

An FM-CW radar front-end was fabricated in an integrated manner at 60 GHz by using the NRD guide. Main emphasis was placed on compactness in size and high-precise operation in performance. The fabricated radar consists of an FM Gunn oscillator, a balanced mixer, and a planar antenna fed by leaky NRD guide with a mechanically beam-scanning performance. All circuit components and the antenna were contained in a compact housing of 170 /spl times/ 140 mm in area and 25 mm in thickness, and thus, a thin type of millimeter-wave radar front-end was successfully developed. Moreover, an error of distance estimation was measured to be less than a distance of 0.7 m.     

型号综合标准化与航空发动机研制

结合航空发动机型号研制,分析了传统型号标准化工作中存在的问题,提出了综合标准化工作的目标和组织体系,探讨了型号综合标准化的工作方法。     

The Evolving Sigmoid: Evidence for Magnetic Flux Ropes in the Corona Before, During, and After CMES

It is generally accepted that the energy that drives coronal mass ejections (CMEs) is magnetic in origin. Sheared and twisted coronal fields can store free magnetic energy which ultimately is released in the CME. We explore the possibility of the specific magnetic configuration of a magnetic flux rope of field lines that twist about an axial field line. The flux rope model predicts coronal observables, including heating along forward or inverse S-shaped, or sigmoid, topological surfaces. Therefore, studying the observed evolution of such sigmoids prior to, during, and after the CME gives us crucial insight into the physics of coronal storage and release of magnetic energy. In particular, we consider (1) soft-X-ray sigmoids, both transient and persistent; (2) The formation of a current sheet and cusp-shaped post-flare loops below the CME; (3) Reappearance of sigmoids after CMEs; (4) Partially erupting filaments; (5) Magnetic cloud observations of filament material.     

Using high-energy proton fluence to improve risk prediction for consequences of solar particle events

The potential for exposure to large solar particle events (SPEs) with high energy levels is a major concern during interplanetary transfer and extra-vehicular activities (EVAs) on the lunar and Mars surface. Previously, we have used data from the last 5 solar cycles to estimate percentiles of dose to a typical blood-forming organ (BFO) for a hypothetical astronaut in a nominally shielded spacecraft during a 120-d lunar mission. As part of this process, we made use of complete energy spectra for 34 large historical SPEs to calculate what the BFO mGy-Eq dose would have been in the above lunar scenario for each SPE. From these calculated doses, we then developed a prediction model for BFO dose based solely on an assumed value of integrated fluence above 30 MeV (Φ₃₀) for an otherwise unspecified future SPE. In this study, we reasoned that since BFO dose is determined more by protons with higher energies than by those with lower energies, more accurate BFO dose prediction models could be developed using integrated fluence above 60 (Φ₆₀) and above 100 MeV (Φ₁₀₀) as predictors instead of Φ₃₀. However to calculate the unconditional probability of a BFO dose exceeding a pre-specified limit (“BFO dose risk”), one must also take into account the distribution of the predictor (Φ_30,Φ₆₀, or Φ₁₀₀), as estimated from historical SPEs. But Φ₆₀ and Φ₁₀₀ have more variability, and less available historical information on which to estimate their distributions over many SPE occurrences, than does Φ₃₀. Therefore, when estimating BFO dose risk there is a tradeoff between increased BFO dose prediction at a given energy threshold and decreased accuracy of models for describing the distribution of that threshold over future SPEs as the threshold increases. Even when taking the second of these two factors into account, we still arrived at the conclusion that overall prediction improves as the energy level threshold increases from 30 to 60 to 100 MeV. These results can be applied to the development of approaches to improve radiation protection of astronauts and the optimization of mission planning for future space missions.