10Be Production in the Atmosphere by Galactic Cosmic Rays

Galactic cosmic ray nuclei and energetic protons produced in solar flares and accelerated by coronal mass ejections are the main sources of high-energy particles of extraterrestrial origin in near-Earth space and inside the Earth’s atmosphere. The intensity of galactic cosmic rays inside the heliosphere is strongly influenced by the modulation of the interstellar source particles on their way through interplanetary space. Among others, this modulation depends on the activity of the Sun, and the resulting intensity of the energetic particles in the atmosphere is an indicator of the solar activity. Therefore, rare isotopes found in historical archives and produced by spallation reactions of primary and secondary hadrons of cosmic origin in the atmosphere, so-called cosmogenic nuclides, can be used to reconstruct the solar activity in the past. The production rate of ¹⁰Be, one of the cosmogenic nuclides most adequate to study the solar activity, is presented showing its variations with geographic latitude and altitude and the dependence on different production cross-sections present in literature. In addition, estimates for altitude integrated production rates of ¹⁰Be at different locations since the early nineteen sixties are shown.     

Effects of lunar and mars dust simulants on HaCaT keratinocytes and CHO-K1 fibroblasts

Exposure to lunar dust during Apollo missions resulted in occasional reports of ocular, respiratory and dermal irritations which showed that lunar dust has a risk potential for human health. This is caused by its high reactivity as well as its small size, leading to a wide distribution also inside habitats. Hence, detailed information regarding effects of extraterrestrial lunar dusts on human health is required to best support future missions to moon, mars or other destinations. In this study, we used several methods to assess the specific effects of extraterrestrial dusts onto mammalian skin by exposing HaCaT keratinocytes and CHO-K1 fibroblasts to dusts simulating lunar or mars soils. These particular cell types were chosen because the skin protects the human body from potentially harmful substances and because a well orchestrated program ensures proper wound healing. Keratinocytes and fibroblasts were exposed to the dusts for different durations of time and their effects on morphology and viability of the cells were determined. Cytotoxicity was measured using the MTT assay and by monitoring culture impedance, while phalloidin staining of the actin cytoskeleton was performed to address structural integrity of the cells which was also investigated by propidium iodide intake. It was found that the effects of the two types of dust simulants on the different features of both cell lines varied to a considerable extent. Moreover, proliferation of HaCaT keratinocytes, as analyzed by Ki67 labeling, was suppressed in sub-confluent cultures exposed to lunar dust simulant. Furthermore, experimental evidence is provided for a delay in regeneration of keratinocyte monolayers from scratch-wounding when exposed to lunar dust simulant. The obtained results will facilitate further investigations of dust exposure during wound healing and will ease risk assessment studies e.g., for lunar lander approaches. The investigations will help to determine safety measures to be taken during extraterrestrial expeditions in order to minimize risks to human health associated with exposure of human skin to dust contaminants.