A role of cytoskeletal structure of cortical cells in the gravity-regulated formation of a peg in cucumber seedlings.

Seedlings of cucurbitaceous plants develop a protuberant tissue, or peg, on the lower side of the transition region between root and hypocotyl when germinated in a horizontal position. Peg develops due to a change in growth polarity of the cortical cells. We have examined the role of the cytoskeletal structure in peg formation of cucumber seedlings. We observed that in both peg and normal cortical cells of 36 h-old seedlings the microtubules (MTs) were arranged perpendicular to the longitudinal axis of the elongating cells. Application of colchicine perturbed the MTs structure and inhibited the formation of pegs. In 20 h-old seedlings, MTs in cortical cells destined to be a peg tissue had no preferential organization, whereas MTs in normal cortical cells were transversely oriented. After 24 h, the MTs in future peg cells were arranged similar to those of 36 h-old seedlings, although the initiation of peg tissue was not yet visible. These results suggest that reorganization of MTs is required for peg formation and causes the change in growth polarity of the cortical cells.     

Agravitropic mutant for the study of hydrotropism in seedling roots

Roots have been shown to respond to a moisture gradient by positive hydrotropism. Agravitropic mutant plants are useful for the study of the hydrotropism in roots because on Earth hydrotropism is obviously altered by the gravity response in the roots of normally gravitropic plants. The roots are able to sense water potential gradient as small as 0.5 MPa mm⁻¹. The root cap includes the sensing apparatus that causes a differential growth at the elongation region of roots. A gradient in apoplastic calcium and calcium influx through plasmamembrane in the root cap is somehow involved in the signal transduction mechanism in hydrotropism, which may cause a differential change in cell wall extensibility at the elongation region. We have isolated an endoxy loglucan transferase (EXGT) gene that is strongly expressed in pea roots and appears to be involved in the differential growth in hydrotropically responding roots. Thus, it is now possible to study hydrotropism in roots by comparing with or separate from gravitropism. These results also imply that microgravity conditions in space are useful for the study of hydrotropism and its interaction with gravitropism.     

JUXTA: A new probe of X-ray emission from the Jupiter system

For the future Japanese exploration mission of the Jupiter’s magnetosphere (JMO: Jupiter Magnetospheric Orbiter), a unique instrument named JUXTA (Jupiter X-ray Telescope Array) is being developed. It aims at the first in-situ measurement of X-ray emission associated with Jupiter and its neighborhood. Recent observations with Earth-orbiting satellites have revealed various X-ray emission from the Jupiter system. X-ray sources include Jupiter’s aurorae, disk emission, inner radiation belts, the Galilean satellites and the Io plasma torus. X-ray imaging spectroscopy can be a new probe to reveal rotationally driven activities, particle acceleration and Jupiter–satellite binary system. JUXTA is composed of an ultra-light weight X-ray telescope based on micromachining technology and a radiation-hard semiconductor pixel detector. It covers 0.3–2 keV with the energy resolution of <100 eV at 0.6 keV. Because of proximity to Jupiter (∼30 Jovian radii at periapsis), the image resolution of <5 arcmin and the on-axis effective area of >3 cm² at 0.6 keV allow extremely high photon statistics and high resolution observations.