Morphological Biosignatures in Early Terrestrial and Extraterrestrial Materials

Biosignatures in early terrestrial rocks are highly relevant in the search for traces of life on Mars because the early geological environments of the two planets were, in many respects, similar and, thus, the potential habitats for early life forms were similar. However, the identification and interpretation of biosignatures in ancient terrestrial rocks has proven contentious over the last few years. Recently, new investigations using very detailed field studies combined with highly sophisticated analytical techniques have begun to document a large range of biosignatures in Early Archaean rocks. Early life on Earth was diversified, widespread and relatively evolved, but its traces are generally, but not always, small and subtle. In this contribution I use a few examples of morphological biosignatures from the Early-Mid Archaean to demonstrate their variety in terms of size and type: macroscopic stromatolites from the 3.443 Ga Strelley Pool Chert, Pilbara; a meso-microscopic microbial mat from the 3.333 Ga Josefsdal Chert, Barberton; microscopic microbial colonies and a biofilm from the 3.446 Ga Kitty’s Gap Chert, Pilbara; and microscopic microbial corrosion pits in the glassy rinds of 3.22–3.48 Ga pillow lavas from Barberton. Some macroscopic and microscopic structures may be identifiable in an in situ robotic mission to Mars and in situ methods of organic molecule detection may be able to reveal organic traces of life. However, it is concluded that it will probably be necessary to return suitably chosen Martian rocks to Earth for the reliable identification of signs of life, since multiple observational and analytical methods will be necessary, especially if Martian life is significantly different from terrestrial life.     

The PROCESS experiment: an astrochemistry laboratory for solid and gaseous organic samples in low-earth orbit

The PROCESS (PRebiotic Organic ChEmistry on the Space Station) experiment was part of the EXPOSE-E payload outside the European Columbus module of the International Space Station from February 2008 to August 2009. During this interval, organic samples were exposed to space conditions to simulate their evolution in various astrophysical environments. The samples used represent organic species related to the evolution of organic matter on the small bodies of the Solar System (carbonaceous asteroids and comets), the photolysis of methane in the atmosphere of Titan, and the search for organic matter at the surface of Mars. This paper describes the hardware developed for this experiment as well as the results for the glycine solid-phase samples and the gas-phase samples that were used with regard to the atmosphere of Titan. Lessons learned from this experiment are also presented for future low-Earth orbit astrochemistry investigations.     

Potential fossil endoliths in vesicular pillow basalt, Coral Patch Seamount, eastern North Atlantic Ocean

The chilled rinds of pillow basalt from the Ampère-Coral Patch Seamounts in the eastern North Atlantic were studied as a potential habitat of microbial life. A variety of putative biogenic structures, which include filamentous and spherical microfossil-like structures, were detected in K-phillipsite-filled amygdules within the chilled rinds. The filamentous structures (～2.5 μm in diameter) occur as K-phillipsite tubules surrounded by an Fe-oxyhydroxide (lepidocrocite) rich membranous structure, whereas the spherical structures (from 4 to 2 μm in diameter) are associated with Ti oxide (anatase) and carbonaceous matter. Several lines of evidence indicate that the microfossil-like structures in the pillow basalt are the fossilized remains of microorganisms. Possible biosignatures include the carbonaceous nature of the spherical structures, their size distributions and morphology, the presence and distribution of native fluorescence, mineralogical and chemical composition, and environmental context. When taken together, the suite of possible biosignatures supports the hypothesis that the fossil-like structures are of biological origin. The vesicular microhabitat of the rock matrix is likely to have hosted a cryptoendolithic microbial community. This study documents a variety of evidence for past microbial life in a hitherto poorly investigated and underestimated microenvironment, as represented by the amygdules in the chilled pillow basalt rinds. This kind of endolithic volcanic habitat would have been common on the early rocky planets in our Solar System, such as Earth and Mars. This study provides a framework for evaluating traces of past life in vesicular pillow basalts, regardless of whether they occur on early Earth or Mars.     

Prebiotic significance of extraterrestrial ice photochemistry: detection of hydantoin in organic residues

The delivery of extraterrestrial organic materials to primitive Earth from meteorites or micrometeorites has long been postulated to be one of the origins of the prebiotic molecules involved in the subsequent apparition of life. Here, we report on experiments in which vacuum UV photo-irradiation of interstellar/circumstellar ice analogues containing H(2)O, CH(3)OH, and NH(3) led to the production of several molecules of prebiotic interest. These were recovered at room temperature in the semi-refractory, water-soluble residues after evaporation of the ice. In particular, we detected small quantities of hydantoin (2,4-imidazolidinedione), a species suspected to play an important role in the formation of poly- and oligopeptides. In addition, hydantoin is known to form under extraterrestrial, abiotic conditions, since it has been detected, along with various other derivatives, in the soluble part of organic matter of primitive carbonaceous meteorites. This result, together with other related experiments reported recently, points to the potential importance of the photochemistry of interstellar "dirty" ices in the formation of organics in Solar System materials. Such molecules could then have been delivered to the surface of primitive Earth, as well as other telluric (exo-) planets, to help trigger first prebiotic reactions with the capacity to lead to some form of primitive biomolecular activity.     

The Emergence of Life

The Rosetta observations have greatly advanced our knowledge of the cometary nucleus and its immediate environment. However, constraints on the mission (both planned and unplanned), the only partially successful Philae lander, and other instrumental issues have inevitably resulted in open questions. Surprising results from the many successful Rosetta observations have also opened new questions, unimagined when Rosetta was first planned. We discuss these and introduce several mission concepts that might address these issues. It is apparent that a sample return mission as originally conceived in the 1980s during the genesis of Rosetta would provide many answers but it is arguable whether it is technically feasible even with today’s technology and knowledge. Less ambitious mission concepts are described to address the suggested main outstanding scientific goals.

     

Mars exploration program analysis group goal one: determine if life ever arose on Mars

The Mars Exploration Program Analysis Group (MEPAG) maintains a standing document that articulates scientific community goals, objectives, and priorities for mission-enabled Mars science. Each of the goals articulated within the document is periodically revisited and updated. The astrobiology-related Goal One, "Determine if life ever arose on Mars," has recently undergone such revision. The finalized revision, which appears in the version of the MEPAG Goals Document posted on September 24, 2010, is presented here.     

The Geology and Habitability of Terrestrial Planets: Fundamental Requirements for Life

The current approach to the study of the origin of life and to the search for life elsewhere is based on two assumptions. First, life is a purely physical phenomenon closely linked to specific environmental conditions. From this, we hypothesise that when these environmental conditions are met, life will arise and evolve. If these assumptions are valid, the search for life elsewhere should be a matter of mapping what we know about the range of environments in which life can exist, and then simply trying to find these environments elsewhere. Second, life can be clearly distinguished from the non-living world. While a single feature of a living organism left in the rock record is not always sufficient to determine unequivocally whether life was present, life often leaves multiple structural, mineralogical and chemical biomarkers that, in sum, support a conclusion that life was present. Our understanding of the habitats that can sustain or have sustained life has grown tremendously with the characterisation of extremophiles. In this chapter, we highlight the range of environments that are known to harbour life on Earth, describe the environments that existed during the period of time when life originated on Earth, and compare these habitats to the suitable environments that are found elsewhere in our solar system, where life could have arisen and evolved.     

The PROCESS experiment: exposure of amino acids in the EXPOSE-E experiment on the international space station and in laboratory simulations

To understand the chemical behavior of organic molecules in the space environment, amino acids and a dipeptide in pure form and embedded in meteorite powder were exposed in the PROCESS experiment in the EXPOSE-E facility mounted on the European Technology Exposure Facility (EuTEF) platform on board the International Space Station (ISS). After exposure to space conditions for 18 months, the samples were returned to Earth and analyzed in the laboratory for reactions caused by solar UV and cosmic radiation. Chemical degradation and possible racemization and oligomerization, the main reactions caused by photochemistry in the vacuum ultraviolet domain (VUV, wavelength range 100-200?nm for photon energy from 6.2 to 12.4?eV) were examined in particular. The molecules were extracted and derivatized by silylation and analyzed by gas chromatograph coupled to a mass spectrometer (GC-MS) to quantify the rate of the degradation of the compounds. Laboratory exposure in several wavelength ranges from UV to VUV was carried out in parallel in the Cologne Deutsches Zentrum für Luft- und Raumfahrt (DLR) Center and Centre de biophysique moléculaire (CBM) laboratories. The results show that resistance to irradiation is a function of the chemical nature of the exposed molecules and the wavelengths of the UV light. The most altered compounds were the dipeptide, aspartic acid, and aminobutyric acid. The most resistant were alanine, valine, glycine, and aminoisobutyric acid. Our results also demonstrate the protective effect of meteorite powder, which reemphasizes the importance of exogenic contribution to the inventory of prebiotic organics on early Earth.     

The Importance of Water for Life

Liquid water is essential for life as we know it, i.e. carbon-based life. Although other compound-solvent pairs that could exist in very specific physical environments could be envisaged, the elements essential to carbon and water-based life are among the most common in the universe. Carbon molecules and liquid water have physical and chemical properties that make them optimised compound-solvent pairs. Liquid water is essential for important prebiotic reactions. But equally important for the emergence of life is the contact of carbon molecules in liquid water with hot rocks and minerals. We here review the environmental conditions of the early Earth, as soon as it had liquid water at its surface and was habitable. Basing our approach to life as a “cosmic phenomenon” (de Duve 1995), i.e. a chemical continuum, we briefly address the various hypotheses for the origin of life, noting their relevance with respect to early environmental conditions. It appears that hydrothermal environments were important in this respect. We continue with the record of early life noting that, by 3.5 Ga, when the sedimentary environment started being well-preserved, anaerobic life forms had colonised all habitable microenvironments from the sea floor to exposed beach environments and, possibly, in the photic planktonic zone of the sea. Life on Earth had also evolved to the relatively sophisticated stage of anoxygenic photosynthesis. We conclude with an evaluation of the potential for habitability and colonisation of other planets and satellites in the Solar System, noting that the most common life forms in the Solar System and probably in the Universe would be similar to terrestrial chemotrophs whose carbon source is either reduced carbon or CO₂ dissolved in water and whose energy would be sourced from oxidized carbon, H₂, or other transition elements.     

Creating Habitable Zones, at all Scales, from Planets to Mud Micro-Habitats, on Earth and on Mars

The factors that create a habitable planet are considered at all scales, from planetary inventories to micro-habitats in soft sediments and intangibles such as habitat linkage. The possibility of habitability first comes about during accretion, as a product of the processes of impact and volatile inventory history. To create habitability water is essential, not only for life but to aid the continual tectonic reworking and erosion that supply key redox contrasts and biochemical substrates to sustain habitability. Mud or soft sediment may be a biochemical prerequisite, to provide accessible substrate and protection. Once life begins, the habitat is widened by the activity of life, both by its management of the greenhouse and by partitioning reductants (e.g. dead organic matter) and oxidants (including waste products). Potential Martian habitats are discussed: by comparison with Earth there are many potential environmental settings on Mars in which life may once have occurred, or may even continue to exist. The long-term evolution of habitability in the Solar System is considered.