The Electric Antennas for the STEREO/WAVES Experiment

The STEREO/WAVES experiment is designed to measure the electric component of radio emission from interplanetary radio bursts and in situ plasma waves and fluctuations in the solar wind. Interplanetary radio bursts are generated from electron beams at interplanetary shocks and solar flares and are observed from near the Sun to 1 AU, corresponding to frequencies of approximately 16 MHz to 10 kHz. In situ plasma waves occur in a range of wavelengths larger than the Debye length in the solar wind plasma λ _D ≈10 m and appear Doppler-shifted into the frequency regime down to a fraction of a Hertz. These phenomena are measured by STEREO/WAVES with a set of three orthogonal electric monopole antennas. This paper describes the electrical and mechanical design of the antenna system and discusses efforts to model the antenna pattern and response and methods for in-flight calibration.     

Delta-electron emission in fast heavy ion atom collisions.

Biological damages such as mutations, chromosomal aberrations etc. are a consequence of biochemical changes mostly in the DNA. With ionizing radiation, these chemical changes are due to primary ionization events and secondary ionization effects caused by the primarily produced electrons. Differences in the biological response of densely ionizing radiation, like heavy charged particles, in comparison to sparsely ionizing radiation, such as X- or gamma-rays, are mainly due to the differences in the production of the so called delta-electrons. Therefore, the emission process of electrons i.e. the cross section for the primary ionization event as well as the energy and angular distribution of the emitted electrons should be understood in detail. The delta-electron emission processes occuring in fast heavy ion atom collisions are explained qualitatively. The different spectral structures of electron emission arising from either the target or the projectile are explained in terms of simple models of the kinetics of momentum transfer induced by the COULOMB forces. In collisions of very heavy ions with matter, high nuclear COULOMB forces are created. These forces lead to a strong polarization of the electronic states of the participated electrons. The effects of this polarization are discussed.     

High-LET radiation carcinogenesis.

Recent results for neutron radiation-induced tumors are presented to illustrate the complexities of the dose-response curves for high-LET radiation. It is suggested that in order to derive an appropriate model for dose-response curves for the induction of tumors by high-LET radiation it is necessary to take into account dose distribution, cell killing and the susceptibility of the tissue under study. Preliminary results for the induction of Harderian gland tumors in mice exposed to various heavy ion beams are presented. The results suggest that the effectiveness of the heavy ion beams increases with increasing LET. The slopes of the dose-response curves for the different high-LET radiations decrease between 20 and 40 rads and therefore comparisons of the relative effectiveness should be made from data obtained at doses below about 20-30 rads.     

S/WAVES: The Radio and Plasma Wave Investigation on the STEREO Mission

This paper introduces and describes the radio and plasma wave investigation on the STEREO Mission: STEREO/WAVES or S/WAVES. The S/WAVES instrument includes a suite of state-of-the-art experiments that provide comprehensive measurements of the three components of the fluctuating electric field from a fraction of a hertz up to 16 MHz, plus a single frequency channel near 30 MHz. The instrument has a direction finding or goniopolarimetry capability to perform 3D localization and tracking of radio emissions associated with streams of energetic electrons and shock waves associated with Coronal Mass Ejections (CMEs). The scientific objectives include: (i) remote observation and measurement of radio waves excited by energetic particles throughout the 3D heliosphere that are associated with the CMEs and with solar flare phenomena, and (ii) in-situ measurement of the properties of CMEs and interplanetary shocks, such as their electron density and temperature and the associated plasma waves near 1 Astronomical Unit (AU). Two companion papers provide details on specific aspects of the S/WAVES instrument, namely the electric antenna system (Bale et al., Space Sci. Rev., 2007) and the direction finding technique (Cecconi et al., Space Sci. Rev., 2007).     

Parabolic maneuvers of the Swiss Air Force fighter jet F-5E as a research platform for cell culture experiments in microgravity

Long-term sensitivity of human cells to reduced gravity has been supposed since the first Apollo missions and was demonstrated during several space missions in the past. However, little information is available on primary and rapid gravi-responsive elements in mammalian cells. In search of rapid-responsive molecular alterations in mammalian cells, short-term microgravity provided by parabolic flight maneuvers is an ideal way to elucidate such initial and primary effects. Modern biomedical research at the cellular and molecular level requires frequent repetition of experiments that are usually performed in sequences of experiments and analyses. Therefore, a research platform on Earth providing frequent, easy and repeated access to real microgravity for cell culture experiments is strongly desired. For this reason, we developed a research platform onboard the military fighter jet aircraft Northrop F-5E “Tiger II”. The experimental system consists of a programmable and automatically operated system composed of six individual experiment modules, placed in the front compartment, which work completely independent of the aircraft systems. Signal transduction pathways in cultured human cells can be investigated after the addition of an activator solution at the onset of microgravity and a fixative or lysis buffer after termination of microgravity. Before the beginning of a regular military training flight, a parabolic maneuver was executed. After a 1 g control phase, the parabolic maneuver starts at 13,000 ft and at Mach 0.99 airspeed, where a 22 s climb with an acceleration of 2.5g is initiated, following a free-fall ballistic Keplerian trajectory lasting 45 s with an apogee of 27,000 ft at Mach 0.4 airspeed. Temperature, pressure and acceleration are monitored constantly during the entire flight. Cells and activator solutions are kept at 37 °C during the entire experiment until the fixative has been added. The parabolic flight profile provides up to 45 s of microgravity at a quality of 0.05g in all axes. Access time is 30 min before take-off; retrieval time is 30 min after landing. We conclude that using military fighter jets for microgravity research is a valuable tool for frequent and repeated cell culture experiments and therefore for state-of-the art method of biomedical research.     

Deployment Mechanisms on the Fast Satellite: Magnetometer,Radial Wire,and Axial Booms

The Fast Auroral SnapshoT (FAST) satellite was launched by a Pegasus XL on August 21, 1996. This was the second launch in the NASA SMall EXplorer (SMEX) program. Early in the mission planning the decision was made to have the University of California at Berkeley Space Sciences Laboratory (UCB-SSL) mechanical engineering staff provide all of the spacecraft appendages, in order to meet the short development schedule, and to insure compatibility. This paper describes the design development, testing and on-orbit deployment of these boom systems: the 2 m carbon fiber magnetometer booms, the 58 m tip to tip spin-plane wire booms, and the 7 m dipole axial stiff booms.     

The STEREO IMPACT Boom

An essential component of the STEREO IMPACT investigation is its nearly 6 m long boom that provides several of the instruments with a sufficiently clean magnetic environment and minimally restricted fields of view, while having the required rigidity to ensure the spacecraft pointing accuracy for the STEREO imaging investigations. Details of the customized telescoping IMPACT Boom design, construction and testing are described in this review. The successful completion and verification of the IMPACT Booms represents a demonstration of the use of Stacers as motive forces for rigid boom deployment.     

Severe disruption of the cytoskeleton and immunologically relevant surface molecules in a human macrophageal cell line in microgravity—Results of an in vitro experiment on board of the Shenzhou-8 space mission

During spaceflight the immune system is one of the most affected systems of the human body. During the SIMBOX (Science in Microgravity Box) mission on Shenzhou-8, we investigated microgravity-associated long-term alterations in macrophageal cells, the most important effector cells of the immune system. We analyzed the effect of long-term microgravity on the cytoskeleton and immunologically relevant surface molecules. Human U937 cells were differentiated into a macrophageal phenotype and exposed to microgravity or 1g on a reference centrifuge on-orbit for 5 days. After on-orbit fixation, the samples were analyzed with immunocytochemical staining and confocal microscopy after landing. The unmanned Shenzhou-8 spacecraft was launched on board a Long March 2F (CZ-2F) rocket from the Jiuquan Satellite Launch Center (JSLC) and landed after a 17-day-mission. We found a severely disturbed actin cytoskeleton, disorganized tubulin and distinctly reduced expression of CD18, CD36 and MHC-II after the 5 days in microgravity. The disturbed cytoskeleton, the loss of surface receptors for bacteria recognition, the activation of T lymphocytes, the loss of an important scavenger receptor and of antigen-presenting molecules could represent a dysfunctional macrophage phenotype. This phenotype in microgravity would be not capable of migrating or recognizing and attacking pathogens, and it would no longer activate the specific immune system, which could be investigated in functional assays. Obviously, the results have to be interpreted with caution as the model system has some limitations and due to numerous technical and biological restrictions (e.g. 23 °C and no CO₂ supply during in-flight incubation). All parameter were carefully pre-tested on ground. Therefore, the experiment could be adapted to the experimental conditions available on Shenzhou-8.     

T cell regulation in microgravity – The current knowledge from in vitro experiments conducted in space,parabolic flights and ground-based facilities

Dating back to the Apollo and Skylab missions, it has been reported that astronauts suffered from bacterial and viral infections during space flight or after returning to Earth. Blood analyses revealed strongly reduced capability of human lymphocytes to become active upon mitogenic stimulation. Since then, a large number of in vitro studies on human immune cells have been conducted in space, in parabolic flights, and in ground-based facilities. It became obvious that microgravity affects cell morphology and important cellular functions. Observed changes include cell proliferation, the cytoskeleton, signal transduction and gene expression. This review gives an overview of the current knowledge of T cell regulation under altered gravity conditions obtained by in vitro studies with special emphasis on the cell culture conditions used. We propose that future in vitro experiments should follow rigorous standardized cell culture conditions, which allows better comparison of the results obtained in different flight- and ground-based experiment platforms.