A viable microbial community in a subglacial volcanic crater lake, Iceland

We describe a viable microbial community in a subglacial lake within the Grímsv?tn volcanic caldera, Iceland. We used a hot water drill to penetrate the 300-m ice shelf and retrieved lake water and volcanic tephra sediments. We also acquired samples of borehole water before and after penetration to the lake, overlying glacial ice and snow, and water from a nearby subaerial geothermal lake for comparative analyses. Lake water is at the freezing point and fresh (total dissolved solids = 260 mg L(-1)). Detectable numbers of cells were found in samples of the lake water column and tephra sediments: 2 x 10(4) ml(-1) and 4 x 10(7) g(-1), respectively. Plate counts document abundant cold-adapted cultivable organisms in the lake water, but not in the borehole (before penetration) or glacial ice. Denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene fragments amplified from genomic DNA extracted from Grímsv?tn samples indicates that the lake community is distinct from the assemblages of organisms in borehole water (before penetration) and the overlying ice and snow. Sequencing of selected DGGE bands revealed that many sequences are highly similar to known psychrophilic organisms or cloned DNA from other cold environments. Significant uptake of 14C-labeled bicarbonate occurred in dark, low-temperature incubations of lake water samples, indicating the presence of autotrophs. Acetylene reduction assays under similar incubation conditions showed no significant nitrogen fixation potential by lake water samples. This may be a consequence of the inhibition of diazotrophy by nitrogen in the lake.     

The origin of organic matter in the Martian meteorite ALH84001.

Stable carbon isotope measurements of the organic matter associated with the carbonate globules and the bulk matrix material in the ALH84001 Martian meteorite indicate that two distinct sources are present in the sample. The delta 13C values for the organic matter associated with the carbonate globules averaged -26% and is attributed to terrestrial contamination. In contrast, the delta 13C values for the organic matter associated with the bulk matrix material yielded a value of -15%. The only common carbon sources on the Earth that yield similar delta 13C values, other then some diagenetically altered marine carbonates, are C4 plants. A delta 13C value of -15%, on the other hand, is consistent with a kerogen-like component, the most ubiquitous form of organic matter found in carbonaceous chondrites such as the Murchison meteorite. Examination of the carbonate globules and bulk matrix material using laser desorption mass spectrometry (LDMS) indicates the presence of a high molecular weight organic component which appears to be extraterrestrial in origin, possibly derived from the exogenous delivery of meteoritic or cometary debris to the surface of Mars.     

Dynamically adaptable m-best 2-D assignment algorithm andmultilevel parallelization

In recent years, there has been considerable interest within the tracking community in an approach to data association based on the m-best two-dimensional (2D) assignment algorithm. Much of the interest has been spurred by its ability to provide various efficient data association solutions, including joint probabilistic data association (JPDA) and multiple hypothesis tracking (MHT). The focus of this work is to describe several recent improvements to the m-best 2D assignment algorithm. One improvement is to utilize a nonintrusive 2D assignment algorithm switching mechanism, based on a problem sparsity threshold. Dynamic switching between two different 2D assignment algorithms, highly suited for sparse and dense problems, respectively, enables more efficient solutions to the numerous 2D assignment problems generated in the m-best 2D assignment framework. Another improvement is to utilize a multilevel parallelization enabling many independent and highly parallelizable tasks to be executed concurrently, including 1) solving the multiple 2D assignment problems via a parallelization of the m-best partitioning task, and 2) calculating the numerous gating tests, state estimates, covariance calculations, and likelihood function evaluations (used as cost coefficients in the 2D assignment problem) via a parallelization of the data association interface task. Using both simulated data and an air traffic surveillance (ATS) problem based on data from two Federal Aviation Administration (FAA) air traffic control radars, we demonstrate that efficient solutions to the data association problem are obtainable using our improvements in the m-best 2D assignment algorithm     

Raman Spectroscopy—A Powerful Tool for in situ Planetary Science

This paper introduces Raman spectroscopy and discusses various scenarios where it might be applied to in situ planetary missions. We demonstrate the extensive capabilities of Raman spectroscopy for planetary investigations and argue that this technique is essential for future planetary missions.