Properties of electrophoretic fractions of human embryonic kidney cells separated on Space Shuttle flight STS-8.

Suspensions of cultured primary human embryonic kidney cells were subjected to continuous flow electrophoresis on Space Shuttle flight STS-8. The objectives of the experiments were to obtain electrophoretically separated fractions of the original cell populations and to test these fractions for the amount and kind of urokinase (a kidney plasminogen activator that is used medically for digesting blood clots), the morphologies of cells in the individual fractions, and their cellular electrophoretic mobilities after separation and subsequent proliferation. Individual fractions were successfully cultured after return from orbit, and they were found to differ substantially from one another and from the starting sample with respect to all of these properties.     

The NASA astrobiology program

The new discipline of astrobiology addresses fundamental questions about life in the universe: "Where did we come from?" "Are we alone in the universe?" "What is our future beyond the Earth?" Developing capabilities in biotechnology, informatics, and space exploration provide new tools to address these old questions. The U.S. National Aeronautics and Space Administration (NASA) has encouraged this new discipline by organizing workshops and technical meetings, establishing a NASA Astrobiology Institute, providing research funds to individual investigators, ensuring that astrobiology goals are incorporated in NASA flight missions, and initiating a program of public outreach and education. Much of the initial effort by NASA and the research community was focused on determining the technical content of astrobiology. This paper discusses the initial answer to the question "What is astrobiology?" as described in the NASA Astrobiology Roadmap.     

Electrophoretic separation of kidney and pituitary cells on STS-8.

A Continuous Flow Electrophoresis System (CFES) was used on Space Shuttle flight STS-8 to separate specific secretory cells from suspensions of cultured primary human embryonic kidney cells and rat pituitary cells. The objectives were to isolate the subfractions of kidney cells that produce the largest amounts of urokinase (plasminogen activator), and to isolate the subfractions of rat pituitary cells that secrete growth hormone, prolactin, and other hormones. Kidney cells were separated into more than 32 fractions in each of two electrophoretic runs. Electrophoretic mobility distributions in flight experiments were spread more than the ground controls. Multiple assay methods confirmed that all cultured kidney cell fractions produced some urokinase, and five to six fractions produced significantly more urokinase than the other fractions. Several fractions also produced tissue plasminogen activator. The pituitary cells were separated into 48 fractions in each of the two electrophoretic runs, and the amounts of growth hormone (GH) and prolactin (PRL) released into the medium for each cell fraction were determined. Cell fractions were grouped into eight mobility classes and immunocytochemically assayed for the presence of GH, PRL, ACTH, LH, TSH, and FSH. The patterns of hormone distribution indicate that the specialized cells producing GH and PRL are isolatable due to the differences in electrophoretic mobilities.     

Thermophysics of the planet Mercury

Recent observations of the thermal emission of Mercury at microwave and infrared frequencies now permit a determination of the thermal and electrical properties of the subsurface of the planet. Radar and optical measurements show that the rotation period is 58.65 days, 2/3 of the orbital period. Several negative spectrographic searches verify that the effects of an atmosphere need not be taken into account in computing surface and subsurface temperatures. The observed thermal emission from the planet can then be interpreted from models similar to those developed for study of the Moon but adapted to the peculiar diurnal insolation of Mercury. The observations of Epstein et al. (1970) at 3.3 mm and of Klein (1970a) at 3.75 cm, when interpreted together with recent laboratory measurements of thermal properties of terrestrial and lunar rock powders, indicate that the ratio of electrical to thermal skin depth is 0.9 ± 0.3 times the wavelength in centimeters. Further results of this analysis of the subsurface are: Density = 1.5 ± 0.4 g cm^-3; Electric loss tangent = 0.009 ± 0.004; Inverse thermal inertia = (15 ± 6) × 10^–6 erg^-1 cm² s^1/2 K; Equatorial midnight temperature = 100 ± 15K.The microwave data generally conform to the predictions of the thermophysical models of Mercury developed by Morrison and Sagan (1967), including a suggestion that variations having mean periods of 50 days and 35 days are present in addition to the classical phase effect with period about 116 days. The time-averaged microwave temperature of the planet appears to increase 25 % from millimeter to decimeter wavelengths; this increase suggests that radiation plays an important role in the transport of heat in the subsurface. All of the conclusions of this review indicate that the thermophysical behavior of Mercury closely approximates that expected for the Moon, were it placed in the orbit of Mercury.     

Impacts and evolution: future prospects

The discipline of astrobiology includes the dynamics of biological evolution. One of the major ways that the cosmos influences life is through the catastrophic environmental disruptions caused when comets and asteroids collide with a planet. We now recognize that such impacts have caused mass extinctions and played a major role in determining the evolution of life on Earth. The time-averaged impact flux as a function of projectile energy can be derived from lunar cratering statistics as well as the current population of near Earth asteroids (NEAs). Effects of impacts of various energies can be modeled, using data from historic impacts [such as the Cretaceous-Tertiary (KT) impactor 65 million years ago] and the observed 1994 bombardment of Jupiter by fragments of Comet Shoemaker-Levy 9. It is of particular interest to find from such models that the terrestrial environment is highly vulnerable to perturbation from impacts, so that even such a small event as the KT impact (by a projectile 10-15 km in diameter) can lead to a mass extinction. Similar considerations allow us to model the effects of still smaller (and much more likely) impacts, down to the size of the asteroid that exploded over Tunguska in 1908 (energy approximately 10 megatons). Combining the impact flux with estimates of environmental and ecological effects reveals that the greatest contemporary hazard is associated with impactors near 1 million megatons in energy (approximately 2 km in diameter for an asteroid). The current impact hazard is significant relative to other natural hazards, and arguments can be developed to illuminate a variety of public policy issues. The first priority in any plan for defense against impactors is to survey the population of Earth-crossing NEAs and project their orbits forward in time. This is the purpose of the Spaceguard Survey, which has already found more than half of the NEAs >1 km in diameter. If there is an NEA on a collision course with Earth, it can be discovered and the impact predicted with decades or more of warning. It is then possible to consider how to deflect or disrupt the NEA. Unlike other natural hazards, the impact risk can be largely eliminated, given sufficient advanced knowledge to take action against the threatening projectile.     

Cellular changes in microgravity and the design of space radiation experiments.

Cell metabolism, secretion and cell-cell interactions can be altered during space flight. Early radiobiology experiments have demonstrated synergistic effects of radiation and microgravity as indicated by increased mutagenesis, increased chromosome aberrations, inhibited development, and retarded growth. Microgravity-induced changes in immune cell functions include reduced blastogenesis and cell-mediated, delayed-type hypersensitivity responses, increased cytokine secretions, but inhibited cytotoxic effects and macrophage differentiation. These effects are important because of the high radiosensitivity of immune cells. It is difficult to compare ground studies with space radiation biology experiments because of the complexity of the space radiation environment, types of radiation damage and repair mechanisms. Altered intracellular functions and molecular mechanisms must be considered in the design and interpretation of space radiation experiments. Critical steps in radiocarcinogenesis could be affected. New cell systems and hardware are needed to determine the biological effectiveness of the low dose rate, isotropic, multispectral space radiation and the potential usefulness of radioprotectants during space flight.     

Production and action of cytokines in space.

B6MP102 cells, a continuously cultured murine bone marrow macrophage cell line, were tested for secretion of tumor necrosis factor-alpha and Interleukin-1 during space flight. We found that B6MP102 cells secreted more tumor necrosis factor-alpha and interleukin-1 when stimulated in space with lipopolysaccharide than controls similarly stimulated on earth. This compared to increased secretion of interferon-beta and -gamma by lymphocytes that was measured on the same shuttle flights. Although space flight enhanced B6MP102 secretion of tumor necrosis factor-alpha, an experiment on a subsequent space flight (STS-50) found that cellular cytotoxicity, mediated by tumor necrosis factor-alpha, was inhibited.     

The mitigation, management, and survivability of asteroid/comet impact with Earth

The chances that Earth will collide with a significant near earth object (NEO) within the next century are very small, but such a collision is possible, would be catastrophic, and could happen at any time. Much discussion has been devoted to methods of diverting these objects away from Earth through the use of space technology. However, if these efforts are unsuccessful, we would need to implement effective strategies to survive the event, no matter how cataclysmic. To date, disaster management for various impact scenarios has not been addressed (except in novels and Hollywood films). An impact disaster may be many orders of magnitude greater than any disaster the human species has ever experienced. Initially, technology and experience gained in other large-scale disasters will most likely form the foundation of how these impact events will be managed and classified. Given the size and energy of the projectile, the estimated area of damage, and whether impact effects might be localized or global in nature, we can begin to build basic disaster response scenarios, anticipate public health concerns, and formulate questions in need of answers. Questions we must deal with include: what will be required technologically, sociologically, and medically to survive? What types of evacuation plans and warning systems might be required? Capabilities in need of further investigation include: technological protection strategies related to ‘impact winter’, expanded chemical hazard control methodologies, food storage and production, roles of national governments, and international cooperation. Whatever the magnitude and severity of the event, we must reflect on what we know, what capabilities we can apply, develop or adapt, and seriously investigate what might be done to manage it and survive.