表面活性剂十二烷基磺酸钠(SDS)对核酸-Tb3+体系荧光性质的影响

研究了 SDS对核酸 -Tb3+体系荧光性质的影响。实验结果表明 ,RNA可以直接与 Tb3+ 反应产生荧光 ,而DNA只能在变性后才能与 Tb3+ 反应。SDS存在下 ,DNA与 RNA体系荧光强度分别增加 4.0和 3 .5倍。     

MTS安全分析与应用

安全是评价分布式系统性能的重要指标,它可能在客户层、业务层或数据层实现。在数据层只有低级别安全控制能力的分布式系统中,往往在业务层实现附加的安全控制以保障整个系统的安全。本文分析了微软分布式企业应用框架(WindowsDNA)的业务层组件框架———MTS(Mi crosoftTransactionServer)的安全模型,并探讨了在数据层只有低级别安全控制的WindowsDNA应用系统中如何实现MTS的安全控制。     

Space radiation effects on plant and mammalian cells

The study of the effects of ionizing radiation on organisms is related to different research aims. The current review emphasizes the studies on the effects of different doses of sparsely and densely ionizing radiation on living organisms, with the final purpose of highlighting specific and common effects of space radiation in mammals and plants. This topic is extremely relevant in the context of radiation protection from space environment. The response of different organisms to ionizing radiation depends on the radiation quality/dose and/or the intrinsic characteristics of the living system. Macromolecules, in particular DNA, are the critical targets of radiation, even if there is a strong difference between damages encountered by plant and mammalian cells. The differences in structure and metabolism between the two cell types are responsible for the higher resistance of the plant cell compared with its animal counterpart.     

Effects of space flight exposure on cell growth,tumorigenicity and gene expression in cancer cells

It is well recognized that harsh outer space environment, consisting of microgravity and radiation, poses significant health risks for human cells. To investigate potential effects of the space environment exposure on cancer cells we examined the biological changes in Caski cells carried by the “Shen Zhou IV” spaceship. After exposure for 7 days in spaceflight, 1440 survival subclonal cell lines were established and 4 cell lines were screened. 44F10 and 17E3 were selected because of their increased cell proliferation and tumorigenesis, while 48A9 and 31F2 had slower cytological events. Experiments with cell proliferation assay, flow cytometry, soft agar assay, tumorigenesis assay and DNA microarray analysis have shown that selected cell lines presented multiple biological changes in cell morphology, cell growth, tumorigenicity and gene expression. These results suggest that space environment exposure can make significant biological impact on cancer cells and provide an entry point to find the immunological target of tumorigenesis.