Optimized design of high-efficiency lunar soil sampling tube structure based on stress–strain law

Understanding the influence of the sampling apparatus on in-situ lunar soil lamination information during the sampling process of the direct push-through lunar weathering layer is of great importance. This paper develops a discrete element model for direct push-through lunar weathering layer sampling using the CUG-1A simulated lunar soil developed by the China University of Geosciences as a simulation object and determines the stress–strain law of the inner wall of a sampling tube. A method for optimizing the inner wall structure of a sampling tube based on the stress–strain law is proposed. The structural disturbance of the marker layer and the lunar soil disturbance rate evaluation function are used to assess the degree of disturbance of the laminar information during the sampling process. The results show that the proposed method can effectively reduce the structural disturbance of the lunar soil in-situ laminar information. In addition, it can also optimize the structural information disturbance of the marker layer. The proposed method can decrease the simulated lunar soil disturbance rate from 0.31 to 0.251 and effectively reduce the penetration force load by 15–20% in the direct push-through sampling process.     

烟草丛顶病毒ORF3的克隆及原核表达

利用RT-PCR方法,自烟草丛顶病毒(Tobacco bush top virus,TBTV)龙陵分离物(TBTV-YLLi)的RNA中扩增出ORF3目的片段,并克隆到pMD18-T载体上进行序列分析.结果表明,TBTV-YLLi的ORF3全长714 bp,与TBTV其他分离物的核苷酸相似性和氨基酸相似性分别为98.3%～98.6%和95.4%～95.8%;与同属的花生丛簇病毒(Groundnut rosette virus,GRV)、豌豆耳突花叶病毒2号(Pea enation mosaic virus-2,PEMV-2)和胡萝卜拟斑驳病毒(Carrot mottle mimic virus,CMoMV)的核苷酸相似性分别为65.1%、61.6%和49.7%,氨基酸相似性分别为36.4%、34.6%和16.5%.将ORF3克隆到原核表达载体pET28a(+)上,获得的重组子pET28-ORF3转化大肠杆菌BL21-plysS,使用终浓度为1.0 mmol/L 的IPTG在37 ℃诱导培养4 h时,该融合蛋白表达量最高.融合蛋白经Ni~(2+)亲和柱层析纯化后,SDS-PAGE 电泳表明,融合蛋白相对分子质量约为35 000,与预计的相对分子质量大小相一致.