X-ray induced alterations in the differentiation and mineralization potential of murine preosteoblastic cells

To evaluate the effects of ionizing radiation (IR) on murine preosteoblastic cell differentiation, we directed OCT-1 cells to the osteoblastic lineage by treatment with a combination of β-glycerophosphate (β-GP), ascorbic acid (AA), and dexamethasone (Dex). In vitro mineralization was evaluated based on histochemical staining and quantification of the hydroxyapatite content of the extracellular bone matrix. Expression of mRNA encoding Runx2, transforming growth factor β1 (TGF-β1), osteocalcin (OCN), and p21^CDKN1A was analyzed. Exposure to IR reduced the growth rate and diminished cell survival of OCT-1 cells under standard conditions. Notably, calcium content analysis revealed that deposition of mineralized matrix increased significantly under osteogenic conditions after X-ray exposure in a time-dependent manner. In this study, higher radiation doses exert significant overall effects on TGF-β1, OCN, and p21^CDKN1A gene expression, suggesting that gene expression following X-ray treatment is affected in a dose-dependent manner. Additionally, we verified that Runx2 was suppressed within 24 h after irradiation at 2 and 4 Gy. Although further studies are required to verify the molecular mechanism, our observations strongly suggest that treatment with IR markedly alters the differentiation and mineralization process of preosteoblastic cells.     

Medical survey of European astronauts during Mir missions

This paper reviews the medical operations performed on six European astronauts during seven space missions on board the space station Mir. These missions took place between November 1988 and August 1999, and their duration ranged from 14 days to 189 days. Steps of pre-flight medical selection and flight certification are presented. Countermeasures program used during the flight, as well as rehabilitation program following short and long-duration missions are described. Also reviewed are medical problems encountered during the flight, post-flight physiological changes such as orthostatic intolerance, exercise capacity, blood composition, muscle atrophy, bone density, and radiation exposure.     

Cutting the bread

Why the draft Treaty on the Prevention of the Placement of Weapons in Outer Space, the Threat or Use of Force Against Outer Space Objects (PPWT) will not work - whereas the Code of Conduct for Outer Space Activities may.     

Developments in space activities in Poland

Poland has a long-standing tradition in space activities. Polish institutions have participated as co-investigators in almost all European Space Agency (ESA) science projects, as well as on many other missions. However, the first Polish satellite (PW-SAT) was only launched in 2012. Poland was one of the first Eastern European countries to conclude a Cooperation Agreement with ESA in the peaceful use of outer space; it was signed in 1994 and followed by a second in January 2002. Negotiations on Polish membership in the ESA were started in autumn of 2011, and ended in April 2012. Following ratification of the agreement, Poland officially became the 20th Member State of ESA on 19 November 2012. This article examines how Poland is setting its way as a space nation. It describes recent developments in the Polish space programme, including the road to Poland's full membership in the European Space Agency.     

Precursor to an African Space Agency: Commentary on Dr Peter Martinez “Is there a need for an African Space Agency?”

In 2012 a working group was established to formulate the African space policy and strategy which would lead to the establishment of an African Space Agency (ASA). Dr Peter Martinez asserts in his article “Is there a need for an African Space Agency?” [1] that the arguments that have been posited in support of an ASA are flawed; namely the arguments of the existing example of the European Space Agency (ESA), and that an ASA would lead to fostering competition, synergy, industrial development and capacity building. While this viewpoint agrees that all the perfect conditions may not exist at present for the creation of an ASA, it addresses some of the issues raised by Martinez, and proposes ideas to foster intra-regional cooperation.     

High velocity impact characterization of Al alloys for oblique impacts

This paper describes the experimental and computational analyses of a high velocity aluminum projectile impact on an Al6061-T6 spacecraft inner wall at different oblique angles. Al2017-T4 spherical projectiles of 5.56 mm in diameter and 0.25 g in weight were chosen within the velocity range of 1000±200 m/s due to the limitation of the light gas gun. The energy absorbed was calculated by measuring the velocities before and after impact on the inner wall. The energy absorbed by the wall and the remaining energy carried by the projectile helped to estimate the severity of further damage to inner components. Afterwards, validation was done by using the commercially available software LS-DYNA with a dedicated SPH. On average, a 10% energy absorption difference between experimentation and simulation was found. By using C-SCAN, the damage area proportion of the total inner wall to impact penetration hole area was found to be on average 6%, 26% and 53% greater than the projectile cross sectional area for the oblique angle impacts of 30°, 45°, and 60°, respectively. These findings helped to understand the relationship between the oblique impact event and the damage area on a spacecraft inner wall along with space debris cloud propagation and comparison with experimental results using LS-DYNA.     

航天飞行器中舷窗温度场及换热的研究：外部界面处于第三类非线性边界条件

本文采用控制容积法,光线踪迹法结合谱带模型,研究航天飞机返回大气层时舷窗硅玻璃的非稳态复合换热。文章给出了界面S_2处于第三类非线性边界条件下,舷窗硅玻璃内部的温度场和进入机舱内部的热流密度。计算结果表明,如不考虑硅玻璃内部的辐射作用,对温度场影响不太大,但对进入机舱内的热流密度将会产生很大的误差。     

Human Mission from Planet Earth: finding a rationale for exploration of the Moon and Mars

This paper advances scenarios for an evolutionary approach to the establishment of a Human Mission from Planet Earth involving exploration and utilization of the Moon and Mars. Of critical importance are the concepts of robotic–human and Moon–Mars synergies. The paper discusses the rationale variables that have an influence on types of space activities pursued and thus the policy that leads to particular scenarios. In this regard, the technological, scientific, and sociopolitical/socioeconomic impacts and consequences related to a Human Mission from Planet Earth are examined.     

Sodium and potassium uptake of Ulva – Application of marine macro-algae for space agriculture

The treatment of sodium, Na, and potassium, K, presents a challenge in space agriculture material recycling, as humans require Na and plants cannot grow at high Na concentrations. Hence, we are proposing the use of marine macro-algae to harvest K and other minerals from composted human waste. Ulva was selected for this feasibility study, since it tolerates a wide range of salinity levels. Growth capability of Ulva was examined under various total salinity levels and proportions of Na and K in the incubation medium. A homeostatic feature of Ulva was found in its intra-cellular concentration of Na and K, and in the intra-cellular ratio between Na and K (at 0.58 ± 0.30, lower than that of human metabolic waste). Intracellular concentration of K in Ulva is 20 times higher than seawater. Because of these characteristics, Ulva is a good candidate species for space agriculture.     

Seasonal dependence of TEC and SLAB thickness

Several years of hourly daily GPS measurements of the vertical total electron content (TEC) and of the equivalent slab thickness made at different European locations are analysed by using the linear regression technique to demonstrate the response of these two ionospheric parameters to seasonal variations. It is found that both TEC and slab thickness are highly correlated with season. Analytical relationships are determined expressing the seasonal dependence of the vertical TEC and of the equivalent slab thickness as a function of the seasonal parameter cos χ at noon in each location.