Current precise point positioning (PPP) techniques are mainly based on GPS which has been extensively investigated. With the increase of available GLONASS satellites during its revitalization, GLONASS observations were increasingly integrated into GPS-based PPP. Now that GLONASS has reached its full constellation, there will be a wide interest in PPP systems based on only GLONASS since it provides a PPP implementation independent of GPS. An investigation of GLONASS-based PPP will also help the development of GPS and GLONASS combined PPP techniques for improved precision and reliability. This paper presents an observation model for GLONASS-based PPP in which the GLONASS hardware delay biases are addressed. In view of frequently changed frequency channel number (FCN) for GLONASS satellites, an algorithm has been developed to compute the FCN for GLONASS satellites using code and phase observations, which avoids the need to provide the GLONASS frequency channel information during data processing. The observation residuals from GLONASS-based PPP are analyzed and compared to those from GPS-based PPP. The performance of GLONASS-based PPP is assessed using data from 15 globally distributed stations. 相似文献
We have explored the role of Ca2+ signaling in microfilament reorganization of osteoblasts induced by simulated weightlessness using a random positioning machine (RPM). The RPM-induced alterations of cell morphology, microfilament distribution, cell proliferation, cell migration, cytosol free calcium concentration ([Ca2+]i), and protein expression in MG63 osteoblasts were investigated. Simulated weightlessness reduced cell size, disrupted microfilament, inhibited cellular proliferation and migration, and induced an increase in [Ca2+]i in MG63 human osteosarcoma cells. Gadolinium chloride (Gd), an inhibitor for stretch-activated channels, attenuated the increase in [Ca2+]i and microfilament disruption. Further, the expression of calmodulin was significantly increased by simulated weightlessness, and an inhibitor of calmodulin, W-7, aggravated microfilament disruption. Our findings demonstrate that simulated weightlessness induces Ca2+ influx through stretch-activated channels, then results in microfilament disruption. 相似文献