Pressure performance for a thin-walled ring and turbulent-jet orifice modeled elastic squeeze film damper

This paper explores an analytical model for Elastic Ring Squeeze Film Damper (ERSFD) with thin-walled ring and turbulent-jet orifices, and uncovers its Oil Film Pressure Performance (OFPP). Firstly, the ring deformation is addressed by using the Fourier series expansion approach and the orifice outflow rate is characterized with the Prandtl boundary layer theory. Secondly, applying finite difference scheme, the influence of elastic ring flexibility, orifice diameter, and attitude angle on the OFPP is analyzed. Finally, Outer chamber pressure was measured experimentally at different rotor speeds. The results indicate that the outer chamber pressure coats an individual load-carrying region and spreads symmetrically pertaining to the attitude angle. Its amplitude drops as the elastic ring flexibility decreases but boosts with the reduction of the orifice diameter. For inner chamber pressure, the orifice diameter effects a similar trend to the outer cavity, but exhibits more stable distribution regarding the attitude angle. Minimizing the elastic ring flexibility causes an increase in amplitude. The model is validated by the test results giving that the outer chamber pressure shifts synchronously and periodically with the variation of the attitude angle, while the pressure amplitude increases slightly at higher rotor speeds.     

烟草丛顶病毒ORF3的克隆及原核表达

利用RT-PCR方法,自烟草丛顶病毒(Tobacco bush top virus,TBTV)龙陵分离物(TBTV-YLLi)的RNA中扩增出ORF3目的片段,并克隆到pMD18-T载体上进行序列分析.结果表明,TBTV-YLLi的ORF3全长714 bp,与TBTV其他分离物的核苷酸相似性和氨基酸相似性分别为98.3%～98.6%和95.4%～95.8%;与同属的花生丛簇病毒(Groundnut rosette virus,GRV)、豌豆耳突花叶病毒2号(Pea enation mosaic virus-2,PEMV-2)和胡萝卜拟斑驳病毒(Carrot mottle mimic virus,CMoMV)的核苷酸相似性分别为65.1%、61.6%和49.7%,氨基酸相似性分别为36.4%、34.6%和16.5%.将ORF3克隆到原核表达载体pET28a(+)上,获得的重组子pET28-ORF3转化大肠杆菌BL21-plysS,使用终浓度为1.0 mmol/L 的IPTG在37 ℃诱导培养4 h时,该融合蛋白表达量最高.融合蛋白经Ni~(2+)亲和柱层析纯化后,SDS-PAGE 电泳表明,融合蛋白相对分子质量约为35 000,与预计的相对分子质量大小相一致.     

基于ICA包络增强MEMD的滚动轴承故障诊断

针对多元经验模态分解(MEMD)存在模态混叠、带内噪声干扰导致轴承故障特征信息微弱难提取问题,提出了基于独立分量分析(ICA)包络增强MEMD的滚动轴承故障诊断.采用MEMD对多通道信号进行自适应分解,依据峭度和相关系数选取包含故障信息的本征模态函数(1MF);对所选取IMF分量的包络信号进行ICA分析,抑制模态混叠和...