Modelling the effects of microgravity on the permeability of air interface respiratory epithelial cell layers

Although it has been suggested that microgravity might affect drug absorption in vivo, drug permeability across epithelial barriers has not yet been investigated in vitro during modelled microgravity. Therefore, a cell culture/diffusion chamber was designed specifically to accommodate epithelial cell layers in a 3D-clinostat and allow epithelial permeability to be measured under microgravity conditions in vitro with minimum alteration to established cell culture techniques. Human respiratory epithelial Calu-3 cell layers were used to model the airway epithelium. Cells grown at an air interface in the diffusion chamber from day 1 or day 5 after seeding on 24-well polyester Transwell cell culture inserts developed a similar transepithelial electrical resistance (TER) to cells cultured in conventional cell culture plates. Confluent Calu-3 layers exposed to modelled microgravity in the 3D-clinostat for up to 48 h maintained their high TER. The permeability of the paracellular marker ¹⁴C-mannitol was unaffected after a 24 h rotation of the cell layers in the 3D-clinostat, but was increased 2-fold after 48 h of modelled microgravity. It was demonstrated that the culture/diffusion chamber developed is suitable for culturing epithelial cell layers and, when subjected to rotation in the 3D-clinostat, will be a valuable in vitro system in which to study the influence of microgravity on epithelial permeability and drug transport.     

A topside investigation over a mid-latitude digisonde station in Cyprus

We examine the systematic differences between topside electron density measurements recorded by different techniques over the low-middle latitude operating European station in Nicosia, Cyprus (geographical coordinates: 35.14^oN, 33.2^oE), (magnetic coordinates 31.86^oN, 111.83 ^oE). These techniques include space-based in-situ data by Langmuir probes on board.European Space Agency (ESA) Swarm satellites, radio occultation measurements on board low Earth orbit (LEO) satellites from the COSMIC/FORMOSAT-3 mission and ground-based extrapolated topside electron density profiles from manually scaled ionograms. The measurements are also compared with International Reference Ionosphere Model (IRI-2016) topside estimations and IRI-corrected NeQuick topside formulation (method proposed by Pezzopane and Pignalberi (2019)). The comparison of Swarm and COSMIC observations with digisonde and IRI estimations verifies that in the majority of cases digisonde underestimates while IRI overestimates Swarm observations but in general, IRI provides a better topside representation than the digisonde. For COSMIC and digisonde profiles matched at the F layer peak the digisonde systematically underestimates topside COSMIC electron density values and the relative difference between COSMIC and digisonde increases with altitude (above hmF2), while IRI overestimates the topside COSMIC electron density but after a certain altitude (~150 km above hmF2) this overestimation starts to decrease with altitude. The IRI-corrected NeQuick underestimates the majority of topside COSMIC electron density profiles and relative difference is lower up to approximately 100 km (above the hmF2) and then it increases. The overall performance of IRI-corrected NeQuick improves with respect to IRI and digisonde.     

粒子拖尾长度对高频DPIV测量误差的影响

基于连续激光源的高采样频率DPIV系统在进行曝光时可能会产生粒子拖尾现象。明渠湍流试验和数值模拟试验结果显示，粒子拖尾长度的增加会导致平均流速减小和湍流强度增大。分析表明，导致平均流速误差的原因之一是诊断窗口边缘局部粒子图像的缺失，且图像缺失程度会随着粒子拖尾长度增加而增大，从而使平均速度随粒子拖尾长度增加而减小；粒子拖尾的另一个直接后果是增大了粒子图像的有效粒径，从而导致湍流强度增大。通过增大第二个诊断窗口的尺寸，进行相关函数标准化和相关系数补偿等方法，可以消除粒子拖尾长度引起的误差。     

可变发射率器件中WO_3电致变色研究进展

概述了WO3电致变色材料的基本结构、电致变色机理和伏安特性.给出了WO3薄膜可变发射率器件的结构.介绍了部分典型非晶、多晶和其他形式的WO3电致变色薄膜,以及发射率及变化范围.     

Influence of atmospheric turbulence on planetary transceiver laser ranging

In this paper we investigate the influence of atmospheric turbulence on the performance of the uplink of a planetary transceiver laser ranging system using a single photon detector. We numerically combine the influence of turbulence in the mean intensity profile variations, scintillation, beam-wander induced pointing errors and stochastic time-of-flight variations, using the Hufnagel–Valley turbulence profile to model the ground turbulence behavior. We map the intensity variations due to turbulence to variations in the probability distribution of the arrival time of the 1st photon in a laser pulse, which influences the range measurement error probability distribution. The turbulence models are applied to assess the influence on single-pass range accuracy and precision statistics, as well as the parameter estimation quality of a Phobos Laser Ranging (PLR) mission.     

Lightweight structure of a phase-change thermal controller based on lattice cells manufactured by SLM

Thermal controllers equipped with phase-change materials are widely used for maintaining the moderate temperatures of various electric devices used in spacecraft. Yet, the structures of amounts of thermal controllers add up to such a large value that restricts the employment of scientific devices due to the limit of rocket capacity. A lightweight structure of phase-change thermal controllers has been one of the main focuses of spacecraft design engineering. In this work, we design a lightweight phase-change thermal controller structure based on lattice cells. The structure is manufactured entirely with AlSi10 Mg by direct metal laser melting. The dimensions of the structure are 230 mm × 170 mm × 15 mm, and the mass is 190 g, which is 60% lighter than most traditional structures(500–600 g) with the same dimensions. The 3 D-printed structure can reduce the risk of leakage at soldering manufacture by a welding process. Whether the strength of the designed structure is sufficient is determined through mechanical analysis and experiments. Thermal test results show that the thermal capacity of the lattice-based thermal controller is increased by50% compared to that of traditional controllers with the same volume.     

航空发动机叶片激光冲击处理过程控制研究

分析了在水约束模式下,光路、约束层、吸收层、靶材等主要因素对激光冲击处理过程控制工艺稳定性的影响。通过研究LSP、MIC、GE、RR等公司在航空发动机叶片激光冲击处理稳定性方面的经验,特别是涉及工艺稳定性的约束控制、光路清理、质量控制等技术,结合叶片激光冲击处理的工艺试验,系统地研究了航空发动机叶片激光冲击处理工艺稳定性的关键控制技术。     

沟槽面湍流边界层湍流度分布

应用激光测速(LDV)技术对沟槽面湍流边界层湍流度分布进行了精细的测量,实验结果表明,在湍流边界层区域沟槽面湍流度最大值比光滑面要小,但出现的位置比光滑面要远.此外,紧靠沟槽面,即y++<4内,沟槽面有较大的湍流度,说明沟槽面有使近壁区流动紊动增加的趋势.对于y/δ≥0.06的区域,湍流度可用u′\u=2.3 exp (-0.9y/δ)描述,而在y/δ<0.06内,则有u′/u =36.4y/δ.     

基于广义相对论的轨道摄动下星间激光相位比对

针对纳米到皮米量级星间激光测距,在地心非旋转坐标系(GCRS)下,考虑卫星轨道摄动引起的广义相对论效应,建立了星间单向以及双向星间激光相位比对模型.通过仿真研究了地球主引力场范围内轨道摄动引起的广义相对论效应对星间激光相位比对误差的影响,并通过星间激光相位比对误差计算星间激光测距误差.不同轨道高度的卫星仿真结果表明,对...     

星载激光测高仪辅助卫星摄影测量浅析

激光测高仪可以快速高精度获取地面高程信息,弥补卫星光学遥感影像三维信息获取能力的不足。采用高精度激光测高数据作为控制信息,符合卫星摄影测量尽量减少地面控制点的发展趋势。文章首先介绍了实际卫星立体测绘中难以解决的问题,结合激光测高的特点,设计了星载激光测高仪辅助空中三角测量立体测绘的方案。根据摄影测量观测方程和激光测高仪对地观测方程,以及卫星影像和激光测高数据外方位元素之间的联系,由光束法平差原理建立观测误差方程。对星载激光测高仪进行定位精度理论分析,采用高精度激光测高数据可以作为高程控制,提高高程观测精度。最后对卫星摄影测量数据与星载激光测高数据联合平差仿真实验,实验结果表明定位精度明显提高。