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991.
992.
生物样品的分离纯化是生命科学研究中的重要环节。在常规地面分离方法中,往往用到离心、萃取等操作,然而在空间微重力条件下,常规的方法无法进行,需要采用特定的方法和技术进行研究。以实现空间环境下细胞内生物大分子的分离为目标,研制了一种可以自动化一体化的装置,其先对细胞样品进行清洗、裂解并释放内溶物,然后在超滤分离池中将生物大分子和小分子化合物进行分离。通过对分离获得的大分子物质如蛋白质的回收率来优化仪器装置的最佳运行条件。结果表明,所研制的装置通过泵在一定频率下的切换,可使裂解体系在膜上进行反复的往返振动,从而使裂解液与细胞进行充分的接触,有效提高了细胞裂解的程度,提高了大分子蛋白的回收率,并通过条件优化确定了装置的最佳运行条件。 相似文献
993.
激波速度测量是激波管和激波风洞运行状态的一个重要参数,压电传感器或光学方法测速系统成本高,而传统电离探针在激波马赫数较低、波后温度达不到空气电离程度的情况下无法满足实验要求。提出了一种使用同轴热电偶作为测速探针来测激波速度的方法,弥补了电离探针在激波马赫数较低时的不足。介绍了同轴热电偶探针测速原理,并设计了测量激波速度的系统电路。通过信号放大电路锁定激波冲激信号,触发脉冲信号发生电路,实现了一种单通道、多测点的激波风洞测速系统。分别开展以温度与热流为触发信号的风洞实验,结果表明只有使用热流信号才能满足激波测速的时间要求。 相似文献
994.
Multi-sensor image matching based on salient edges has broad prospect in applications, but it is difficult to extract salient edges of real multi-sensor images with noises fast and accurately by using common algorithms. According to the analysis of the features of salient edges, a novel salient edges detection algorithm and its rapid calculation are proposed based on possibility fuzzy C-means (PFCM) kernel clustering using two-dimensional vectors composed of the values of gray and texture. PFCM clustering can overcome the shortcomings that fuzzy C-means (FCM) cluster- ing is sensitive to noises and possibility C-means (PCM) clustering tends to find identical clusters. On this basis, a method is proposed to improve real-time performance by compressing data sets based on the idea of data reduction in the field of mathematical analysis. In addition, the idea that kernel-space is linearly separable is used to enhance robustness further. Experimental results show that this method extracts salient edges for real multi-sensor images with noises more accurately than the algorithm based on force fields and the FCM algorithm; and the proposed method is on average about 56 times faster than the PFCM algorithm in real time and has better robustness. 相似文献
995.
996.
Aeroelastic two-level optimization for preliminary design of wing structures considering robust constraints 总被引:1,自引:0,他引:1
An aeroelastic two-level optimization methodology for preliminary design of wing struc- tures is presented, in which the parameters for structural layout and sizes are taken as design vari- ables in the first-level optimization, and robust constraints in conjunction with conventional aeroelastic constraints are considered in the second-level optimization. A low-order panel method is used for aerodynamic analysis in the first-level optimization, and a high-order panel method is employed in the second-level optimization. It is concluded that the design of the abovementioned structural parameters of a wing can be improved using the present method with high efficiency. An improvement is seen in aeroelastic performance of the wing obtained with the present method when compared to the initial wing. Since these optimized structures are obtained after consideration of aerodynamic and structural uncertainties, they are well suited to encounter these uncertainties when they occur in reality. 相似文献
997.
This paper presents a two-level geometric calibration method for the permanent magnet (PM) spherical actuator to improve its motion control accuracy. The proposed actuator is com- posed of a stator with circumferential coils and a rotor with multiple PM poles. Due to the assembly and fabrication errors, the real geometric parameters of the actuator will deviate from their design values. Hence, the identification of such errors is critical for the motion control tasks. A two-level geometric calibration approach is proposed to identify such errors. In the first level, the calibration model is formulated based on the differential form of the kinematic equation, which is to identify the geometric errors in the spherical joint. In the second level, the calibration model is formulated based on the differential form of torque formula, which is to calibrate the geometric parameters of the magnetization axes of PM poles and coils axes. To demonstrate the robustness and availability of the calibration algorithm, simulations are conducted. The results have shown that the proposed two-level calibration method can effectively compensate the geometric parameter errors and improve the positioning accuracy of the spherical actuator. 相似文献
998.
Gray bootstrap method for estimating frequency-varying random vibration signals with small samples 总被引:2,自引:0,他引:2
During environment testing, the estimation of random vibration signals (RVS) is an important technique for the airborne platform safety and reliability. However, the available meth- ods including extreme value envelope method (EVEM), statistical tolerances method (STM) and improved statistical tolerance method (ISTM) require large samples and typical probability distri- bution. Moreover, the frequency-varying characteristic of RVS is usually not taken into account. Gray bootstrap method (GBM) is proposed to solve the problem of estimating frequency-varying RVS with small samples. Firstly, the estimated indexes are obtained including the estimated inter- val, the estimated uncertainty, the estimated value, the estimated error and estimated reliability. In addition, GBM is applied to estimating the single flight testing of certain aircraft. At last, in order to evaluate the estimated performance, GBM is compared with bootstrap method (BM) and gray method (GM) in testing analysis. The result shows that GBM has superiority for estimating dynamic signals with small samples and estimated reliability is proved to be 100% at the given confidence level. 相似文献
999.
A seal device as an important component of aeroengines has decisive influence on per- formance, reliability, and working life of aeroengines. With the development of aeroengines, demands on the performance characteristics of seal devices are made strictly. Finger seal as a novel kind of sealing device, recently attracts more and more attentions in academic circles and engineer- ing fields at home and abroad. Research on finger seals has been extensively developed, especially on leakage and wear performances under dynamic conditions. However, it is a pity that the work on finger seals has been limited with a single approach that is improving the performance by structural optimization; in addition, the technology of dynamic analysis on finger seals is weak. Aiming at the problems mentioned above, a distributed mass equivalent dynamic model of finger seals considering the coupling effect of overlaid laminates is established in the present paper, the dynamic perfor- mance of 2.5 dimension C/C composite finger seal is analyzed with the model, and then the effects of fiber bundle density and fiber bundle preparation direction on finger seal's dynamic performance are discussed, as well as compared with those of Co-based alloy finger seal. The current work is about dynamic analysis of finger seals and application of C/C composite in this paper may have much academic significance and many engineering values for improving research level of finger seal dynamics and exploring feasibility of C/C composite being used for finger seals. 相似文献
1000.