Water movement on the convex surfaces of porous media under microgravity

A plant growth system for crop production under microgravity is part of a life supporting system designed for long-duration space missions. A plant growth in soil in space requires the understanding of water movement in soil void spaces under microgravity. Under 1G-force condition, on earth, water movement in porous media is driven by gradients of matric and gravitational potentials. Under microgravity condition, water movement in porous media is supposed to be driven only by a matric potential gradient, but it is still not well understood. We hypothesized that under microgravity water in void spaces of porous media hardly moved comparing in void spaces without obstacles because the concave surfaces of the porous media hindered water movement. The objective of this study was to investigate water movement on the convex surfaces of porous media under microgravity. We conducted parabolic flight experiments that provided 20–25?s of microgravity at the top of a parabolic flight. We observed water movement in void spaces in soil-like porous media made by glass beads and glass spheres (round-bottomed glass flasks) in the different conditions of water injection under microgravity. Without water injection, water did not move much in neither glass beads nor glass spheres. When water was injected during microgravity, water accumulated in contacts between the particles, and the water made thick fluid films on the particles surface. When the water injection was stopped under microgravity, water was held in the contacts between the particles. This study showed that water did not move upward in the void spaces with or without the water injection. In addition, our results suggested that the difficulty of water movement on the convex (i.e. particle surfaces) might result in slower water move in porous media under microgravity than at 1G-force.     

Establishment of a rearing system of the extremotolerant tardigrade Ramazzottius varieornatus: a new model animal for astrobiology

Studies on the ability of multicellular organisms to tolerate specific environmental extremes are relatively rare compared to those of unicellular microorganisms in extreme environments. Tardigrades are extremotolerant animals that can enter an ametabolic dry state called anhydrobiosis and have high tolerance to a variety of extreme environmental conditions, particularly while in anhydrobiosis. Although tardigrades have been expected to be a potential model animal for astrobiological studies due to their excellent anhydrobiotic and extremotolerant abilities, few studies of tolerance with cultured tardigrades have been reported, possibly due to the absence of a model species that can be easily maintained under rearing conditions. We report the successful rearing of the herbivorous tardigrade, Ramazzottius varieornatus, by supplying the green alga Chlorella vulgaris as food. The life span was 35 +/- 16.4 d, deposited eggs required 5.7 +/- 1.1 d to hatch, and animals began to deposit eggs 9 d after hatching. The reared individuals of this species had an anhydrobiotic capacity throughout their life cycle in egg, juvenile, and adult stages. Furthermore, the reared adults in an anhydrobiotic state were tolerant of temperatures of 90 degrees C and -196 degrees C, and exposure to 99.8% acetonitrile or irradiation with 4000 Gy (4)He ions. Based on their life history traits and tolerance to extreme stresses, R. varieornatus may be a suitable model for astrobiological studies of multicellular organisms.     

High resolution spectroscopy of X-ray emission from high mass X-ray binaries

This article briefly reviews first the progress of spectroscopy in X-ray astronomy from proportional counters, a major instrument in early phase of X-ray astronomy, to gas scintillation proportional counters, X-ray CCD cameras, transmission and reflection gratings, and finally to X-ray micro-calorimeters. As a typical example of spectral features observed from high mass X-ray binaries (HMXBs), the spectra observed from Vela X-1 with Chandra grating spectrometers are then presented and compared with computer simulations for high mass binary systems.     

LET dependence of cell death, mutation induction and chromatin damage in human cells irradiated with accelerated carbon ions.

We investigated the LET dependence of cell death, mutation induction and chromatin break induction in human embryo (HE) cells irradiated by accelerated carbon-ion beams. The results showed that cell death, mutation induction and induction of non-rejoining chromatin breaks detected by the premature chromosome condensation (PCC) technique had the same LET dependence. Carbon ions of 110 to 124keV/micrometer were the most effective at all endpoints. However, the number of initially induced chromatin breaks was independent of LET. About 10 to 15 chromatin breaks per Gy per cell were induced in the LET range of 22 to 230 keV/micrometer. The deletion pattern of exons in the HPRT locus, analyzed by the polymerase chain reaction (PCR), was LET-specific. Almost all of the mutants induced by 124 keV/micrometer beams showed deletion of the entire gene, while all mutants induced by 230keV/micrometer carbon-ion beams showed no deletion. These results suggest that the difference in the density distribution of carbon-ion track and secondary electron with various LET is responsible for the LET dependency of biological effects.     

Telescience testbed for biomedical experiments in space morphological and physiological experiments of rat musculoskeletal system.

As the second telescience testbed experiment we were examined sophisticated processes of biomedical experiment, such as an implantation of a transmitter into the hamster's abdominal cavity, non-stressful blood sampling, large amount of blood collection, muscle extirpation and biopsy from the hamsters on February 6-8, 1990. To make clear the differences between successful results obtained by an experienced hand and by a non-experienced one, three operators were selected for three successive experimental days; an engineer who had never experienced any biological experiment, a non-biology student, who experienced on biological experiments, and a veterinary surgeon. Surgical procedures need much experiences on maneuvering and understanding of theory to shorten the elapse time. Especially for a non-experienced hand, graphic instructions were much helpful to understand and to maneuver the procedures. Continuous recordings of ECG from a operator and PIs were of an advantage to grasp an extent of the mental strain, which was compared with their reports requested after end of each experimental day. The mental strain was not related to degrees of scientific achievement, but showed faithfully difficulty of each experimental procedure. Training effects on PIs in successive experimental days were found in their instructions for the operator to let understand the procedures.     

Small cryogenic propulsion unit for upper stage application

The feasibility study was conducted to use the cryogenic propulsion system for the third stage of the future H-1 vehicle. While the $\text{LO}{}_2\text{LH}{}_2$ third-stage mass fraction is less than the current solid propellant third stage, the 50% higher Isp results in a significantly higher payload. Two basic configurations of the new propulsion system were proposed: one pressure-fed system and two pump-fed systems. The first is a pressure-fed system providing a 700 kg thrust at an Isp of 441 sec with restart capability. The second is a pump-fed system, operating on an expander cycle principle. A midget turbopump with a 90 000 rpm shaft speed feeds the thrust chamber which delivers 1 ton of thrust at an Isp of 471 sec. The third proposed system is also a pump-fed design using a unique expander bleed cycle, and delivers a 1 ton thrust at an Isp of 470 sec with a turbopump speed of 80 000 rpm. The results of engine testing predict the performance feasibility of respective propulsion system designs.     

Induction of asymmetrical type of chromosomal aberrations in cultured human lymphocytes by ion beams of different energies at varying let from HIMAC and RRC

Frequencies of asymmetrical type of chromosome aberration were scored in cultured human blood lymphocytes irradiated with carbon and neon beams. Blood cells were irradiated with various doses to establish dose response curves for chromosome aberration frequency vs. dose, and chromosome preparation was made by conventional method. Dose response curves for the per cell frequencies of the dicentrics and centric rings as well as the excess amount of acentric fragments were described for 7 different qualities (LET= 22.4, 40.0, 41.5, 69.9, 70.0, 100.0 and 150 KeV/μm) of carbon and neon beams with three different energies, 135, 290 and 400 MeV/u. From the analysis of those dose response curves, the maximum effect was found in the region of LET value at near 70 KeV/μm together with linear expression in the response from all endpoints examined. The 135 MeV/u of carbons (69.9 KeV/μm) and neons (70.0 KeV/μm) showed linear response. The 290 MeV/u of carbons (100 KeV/m) and neons (150 KeV/μm) showed medium effects with different shape of response, linear with a plateau and upward concavity. The 2 carbon beams (41.5 and 40 KeV/μm) from 2 different accelerators showed much discrepancy in the response. RBE-LET relationship was also described by comparing the coefficient of the 7 different dose responses. The peak (near 70 KeV/m) was localized colse to that (80 KeV/m) for the survivals of dsb repair deficient cells (Eguchi-Kasai et al. 1998), but in different position from that previously reported in many other studies (100–200 KeV/mm). Identification of the RBEmax in the present study has yet to be definitive.     

Changes in prevalence of subjective fatigue during 14-day 6° head-down bed rest

The present study examines the prevalence of subjective fatigue in young healthy males during 14 days of 6° head-down bed rest (HDBR) by using a multidimensional questionnaire. Forty-one subjects completed the Subjective Fatigue Scale questionnaire to assess the fatigue-related complaints and symptoms. The questionnaire is composed of three sections, with 10 items each. The sections measured drowsiness and dullness (Section 1), difficulty in concentration (Section 2), and the projection of physical disintegration (Section 3). The subjects answered simple questions between 1400 and 1700 on 6 measurement days before and during the HDBR period. The prevalence rate of low back pain was markedly high (80.5%) on the second day and more than 50% in the first half of the HDBR period, and any complaints related to either a lack of sleep or a deterioration in the quality of sleep continued until the end of the HDBR period. Our findings may be useful in developing preventive strategies against physical and mental fatigue associated with prolonged HDBR, horizontal bed rest, and microgravity environments.     

Solar neutron events as a tool to study particle acceleration at the Sun

The Sun provides unique opportunities to study particle acceleration mechanisms using data from detectors placed on the Earth’s surface and on board spacecrafts. Particles may gain high energies by several physical mechanisms. Differentiating between these possibilities is a fundamental problem of cosmic ray physics. Energetic neutrons provide us with information that keeps the signatures of the acceleration site. A summary of some representative solar neutron events observed on the Earth’s surface, including associated X and γ-ray observations from spacecrafts is presented. We discuss evidence of acceleration of particles by the Sun to energies up to several tens of GeV. In addition, a recent solar neutron event that occurred on September 7th 2005 and detected by several observatories at Earth is analyzed in detail.     

Hands-on education system using water rocket

Trial of hands-on education of rocket technology for university students using water rocket, which consists of structural study, propulsion system study, aerodynamic study, stability study, and flight trajectory study, has been performed. Integrating each subject into a group of tasks, a hands-on education system of aerospace technology will become feasible. Advantage of the system is that students can verify the theory by hands-on practice. Development of recovery mechanism for water rocket will be a good subject of hands-on innovative design. Although the system has been developed mostly for university students, it can easily be converted to hands-on education system for school children and high school students.