Quality characteristics of the radish grown under reduced atmospheric pressure

This study addresses whether reduced atmospheric pressure (hypobaria) affects the quality traits of radish grown under such environments. Radish (Raphanus sativus L. cv. Cherry Bomb Hybrid II) plants were grown hydroponically in specially designed hypobaric plant growth chambers at three atmospheric pressures; 33, 66, and 96 kPa (control). Oxygen and carbon dioxide partial pressures were maintained constant at 21 and 0.12 kPa, respectively. Plants were harvested at 21 days after planting, with aerial shoots and swollen hypocotyls (edible portion of the radish referred to as the “root” hereafter) separated immediately upon removal from the chambers. Samples were subsequently evaluated for their sensory characteristics (color, taste, overall appearance, and texture), taste-determining factors (glucosinolate and soluble carbohydrate content and myrosinase activity), proximate nutrients (protein, dietary fiber, and carbohydrate) and potential health benefit attributes (antioxidant capacity). In roots of control plants, concentrations of glucosinolate, total soluble sugar, and nitrate, as well as myrosinase activity and total antioxidant capacity (measured as ORAC_FL), were 2.9, 20, 5.1, 9.4, and 1.9 times greater than the amount in leaves, respectively. There was no significant difference in total antioxidant capacity, sensory characteristics, carbohydrate composition, or proximate nutrient content among the three pressure treatments. However, glucosinolate content in the root and nitrate concentration in the leaf declined as the atmospheric pressure decreased, suggesting perturbation to some nitrogen-related metabolism.     

Gas exchange rates of potato stands for bioregenerative life support

Plants can provide a means for removing carbon dioxide (CO₂) while generating oxygen (O₂) and clean water for life support systems in space. To study this, 20 m² stands of potato (Solanum tuberosum L.) plants were grown in a large (113 m³ vol.), atmospherically closed chamber. Photosynthetic uptake of CO₂ by the stands was detected about 10 DAP (days after planting), after which photosynthetic rates rose rapidly as stand ground cover and total light interception increased. Photosynthetic rates peaked ca. 50 DAP near 45 μmol CO₂ m⁻² s⁻¹ under 865 μmol m⁻² s⁻¹ PPF (average photosynthetic photon flux), and near 35 μmol CO₂ m⁻² s⁻¹ under 655 μmol m⁻² s⁻¹ PPF. Short term changes in PPF caused a linear response in stand photosynthetic rates up to 1100 μmol m⁻² s⁻¹ PPF, with a light compensation point of 185 μmol m⁻² s⁻¹ PPF. Comparisons of stand photosynthetic rates at different CO₂ concentrations showed a classic C₃ response, with saturation occurring near 1200 μmol mol⁻¹ CO₂ and compensation near 100 μmol mol⁻¹ CO₂. In one study, the photoperiod was changed from 12 h light/12 h dark to continuous light at 58 DAP. This caused a decrease in net photosynthetic rates within 48 h and eventual damage (scorching) of upper canopy leaves, suggesting the abrupt change stressed the plants and/or caused feedback effects on photosynthesis. Dark period (night) respiration rates increased during early growth as standing biomass increased and peaked near 9 μmol CO₂ m⁻² s⁻¹ ca. 50 DAP, after which rates declined gradually with age. Stand transpiration showed a rapid rise with canopy ground cover and peaked ca. 50 DAP near 8.9 L m⁻² d⁻¹ under 860 μmol m⁻² s⁻¹ PPF and near 6.3 L m⁻² d⁻¹ under 650 μmol m⁻² s⁻¹ PPF. Based on the best photosynthetic rates from these studies, approximately 25 m² of potato plants under continuous cultivation would be required to support the CO₂ removal and O₂ requirements for one person.     

Children's control of reference systems in spatial tasks: Foundations of spatial cognitive skill?

Two experiments are presented, one that focused on the abilities of 3- through 8-year-old children to use frames of reference as means for solving a search task in a small-scale perceived space and one that focused on the abilities of 8- through 11-year-old children to use different frames of reference to answer questions about the locations of objects in an environment they had read about. Experiment 1 revealed developmental improvement in the consistent application of object-, place-, and viewer-based frames of reference, with scale of space impacting the pattern of age-related improvement. Experiment 2 showed that young readers' first successes with switching from one reference system to another in representational space involved a vantage point perspective, a viewer-based ``gaze tour' of a small-scale area. Although disparate in focus and method, the studies point to the need for a new theoretical framework for examining developmental issues in the application of spatial frames of reference.     

Degradation of cyanobacterial biosignatures by ionizing radiation

Primitive photosynthetic microorganisms, either dormant or dead, may remain today on the martian surface, akin to terrestrial cyanobacteria surviving endolithically in martian analog sites on Earth such as the Antarctic Dry Valleys and the Atacama Desert. Potential markers of martian photoautotrophs include the red edge of chlorophyll reflectance spectra or fluorescence emission from systems of light-harvesting pigments. Such biosignatures, however, would be modified and degraded by long-term exposure to ionizing radiation from the unshielded cosmic ray flux onto the martian surface. In this initial study into this issue, three analytical techniques--absorbance, reflectance, and fluorescence spectroscopy--were employed to determine the progression of the radiolytic destruction of cyanobacteria. The pattern of signal loss for chlorophyll reflection and fluorescence from several biomolecules is characterized and quantified after increasing exposures to ionizing gamma radiation. This allows estimation of the degradation rates of cyanobacterial biosignatures on the martian surface and the identification of promising detectable fluorescent break-down products.     

Crystallisation of alpha-crustacyanin, the lobster carapace astaxanthin-protein: results from EURECA.

Crystallisation of alpha-crustacyanin, the lobster carapace astaxanthin-protein was attempted under microgravity conditions in EURECA satellite using liquid-liquid diffusion with polyethyleneglycol (PEG) as precipitant; in a second reaction chamber phenol and dioxan were used as additives to prevent composite crystal growth. Crystals of alpha-crustacyanin grown under microgravity from PEG were larger than those grown terrestrially in the same apparatus under otherwise identical conditions. On retrieval, the crystals from PEG were shown to be composite and gave a powder diffraction pattern. The second reaction chamber showed leakage on retrieval and had also been subjected to rapid temperature variation during flight. Crystal fragments were nevertheless recovered but showed a powder diffraction pattern. It is concluded, certainly for liquid-liquid diffusion using PEG alone, that, for crustacyanin, although microgravity conditions resulted in an increase in dimensions of crystals, a measurable improvement in molecular ordering was not achieved.     

Near term approach to data relay for satellites under test

The increasing need for a continuous communications link with U.S. Department of Defense (DoD) spacecraft during test missions in low Earth orbit (LEG) has resulted in greater interest in geosynchronous data relay services. This may be a more economical alternative to building additional remote tracking stations for the Air Force Satellite Control Network (AFSCN), and avoids tying up operational assets for a test mission. A low-cost near-term approach for such a space-based data relay system would utilize two existing Defense Satellite Communication System III spacecraft, two existing ground terminals, and a small, standardized terminal using autonomous antenna pointing for the space vehicle under test. Such a system design is presented     

Spherule beds 3.47-3.24 billion years old in the Barberton Greenstone Belt,South Africa: a record of large meteorite impacts and their influence on early crustal and biological evolution

Four layers, S1-S4, containing sand-sized spherical particles formed as a result of large meteorite impacts, occur in 3.47-3.24 Ga rocks of the Barberton Greenstone Belt, South Africa. Ir levels in S3 and S4 locally equal or exceed chondritic values but in other sections are at or only slightly above background. Most spherules are inferred to have formed by condensation of impact-produced rock vapor clouds, although some may represent ballistically ejected liquid droplets. Extreme Ir abundances and heterogeneity may reflect element fractionation during spherule formation, hydraulic fractionation during deposition, and/or diagenetic and metasomatic processes. Deposition of S1, S2, and S3 was widely influenced by waves and/or currents interpreted to represent impact-generated tsunamis, and S1 and S2 show multiple graded layers indicating the passage of two or more wave trains. These tsunamis may have promoted mixing within a globally stratified ocean, enriching surface waters in nutrients for biological communities. S2 and S3 mark the transition from the 300-million-year-long Onverwacht stage of predominantly basaltic and komatiitic volcanism to the late orogenic stage of greenstone belt evolution, suggesting that regional and possibly global tectonic reorganization resulted from these large impacts. These beds provide the oldest known direct record of terrestrial impacts and an opportunity to explore their influence on early life, crust, ocean, and atmosphere. The apparent presence of impact clusters at 3.26-3.24 Ga and approximately 2.65-2.5 Ga suggests either spikes in impact rates during the Archean or that the entire Archean was characterized by terrestrial impact rates above those currently estimated from the lunar cratering record.     

Temperature-Driven Segmentation for Autonomous Anti-Tank Weapons

In this paper we discuss a simple temperature-driven segmentation technique that can be used for autonomous terminal guidance of anti-tank munitions. A ratio between two images from different IR bands is taken to form an emissivity independent image that is a function of temperature only. Size and temperature discrimination can then be used to find hot spots of interest. Candidate targets are segmented from the image about the points of interest using an.algorithm that finds all regions in multi-region objects and does not require a priori intensity information. Size and gross shape features are used to determine if the segmented objects are to be classified as targets. The key to this approach is the initial hot spot extraction. The temperature dependent ratio image allows quick and confident screening of the entire field of view, so that only the regions of interest require further processing. The described techniques effectively found tanks in the presence of common battlefield clutter when used with synthetic IR imagery provided by Georgia Tech Research Institute. The simplicity, effectiveness and potential speed of this technique make it ideal for autonomous guidance of expendable anti-tank weapons, especially where only low resolution IR imagery is available.     

Non-BBN Constraints on the Key Cosmological Parameters

Since the baryon-to-photon ratio 10 is in some doubt at present, we ignore the constraints on 10 from big bang nucleosynthesis (BBN) and fit the three key cosmological parameters (h, M, 10) to four other observational constraints: Hubble parameter (ho), age of the universe (to), cluster gas (baryon) fraction (fo fGh3/2), and effective shape parameter (o). We consider open and flat CDM models and flat CDM models, testing goodness of fit and drawing confidence regions by the 2 method. CDM models with M = 1 (SCDM models) are accepted only because we allow a large error on ho, permitting h < 0.5. Open CDM models are accepted only for _M 0.4. CDM models give similar results. In all of these models, large 10 ( 6) is favored strongly over small 10 ( 2), supporting reports of low deuterium abundances on some QSO lines of sight, and suggesting that observational determinations of primordial 4He may be contaminated by systematic errors. Only if we drop the crucial o constraint are much lower values of M and 10 permitted.