Electrophoretic separation of kidney and pituitary cells on STS-8.

A Continuous Flow Electrophoresis System (CFES) was used on Space Shuttle flight STS-8 to separate specific secretory cells from suspensions of cultured primary human embryonic kidney cells and rat pituitary cells. The objectives were to isolate the subfractions of kidney cells that produce the largest amounts of urokinase (plasminogen activator), and to isolate the subfractions of rat pituitary cells that secrete growth hormone, prolactin, and other hormones. Kidney cells were separated into more than 32 fractions in each of two electrophoretic runs. Electrophoretic mobility distributions in flight experiments were spread more than the ground controls. Multiple assay methods confirmed that all cultured kidney cell fractions produced some urokinase, and five to six fractions produced significantly more urokinase than the other fractions. Several fractions also produced tissue plasminogen activator. The pituitary cells were separated into 48 fractions in each of the two electrophoretic runs, and the amounts of growth hormone (GH) and prolactin (PRL) released into the medium for each cell fraction were determined. Cell fractions were grouped into eight mobility classes and immunocytochemically assayed for the presence of GH, PRL, ACTH, LH, TSH, and FSH. The patterns of hormone distribution indicate that the specialized cells producing GH and PRL are isolatable due to the differences in electrophoretic mobilities.     

A liquid propulsion panorama

Liquid-propellant rocket engines are widely used all over the world, thanks to their high performances, in particular high thrust-to-weight ratio. The present paper presents a general panorama of liquid propulsion as a contribution of the IAF Advanced Propulsion Prospective Group.After a brief history of its past development in the different parts of the world, the current status of liquid propulsion, the currently observed trends, the possible areas of future improvement and a summarized road map of future developments are presented. The road map includes a summary of the liquid propulsion status presented in the “Year in review 2007” of Aerospace America.Although liquid propulsion is often seen as a mature technology with few areas of potential improvement, the requirements of an active commercial market and a renewed interest for space exploration has led to the development of a family of new engines, with more design margins, simpler to use and to produce associated with a wide variety of thrust and life requirements.     

A multi-disciplinary plan for easier access, management, and analysis of science data

NASA’s COST LESS Team is pursuing strategies to reduce the cost and complexity of planning and executing space missions. The team’s technical goal is to reverse the trend of constructing unique solutions for similar problems. To this end, the team is exploring ways to represent mission functionality in terms of building blocks and is discovering approaches that could accommodate the same building blocks for seemingly disparate activities, such as organizing processed telemetry data, controlling onboard experiments, searching science archives, reducing and presenting information to science users, and supporting educational outreach. Reusable object technology (UOT), a research undertaking by the authors, is showing promise in recognizing similarities in functions which were previously viewed as unique because they appeared in different programs or mission phases. Since UOT is aimed at being implementation independent (i.e. the function performed could be accomplished manually, by an automated process, by a specialized instrument, etc.), no premature judgment for automation or autonomy need be made. In this paper, the authors attempt to strike a balance between theory and reality as they describe UOT, including its beginnings, its underpinning, its utility, and its potential for achieving substantive reductions in cost and complexity for the Agency’s space programs. The authors discuss their collaboration with the Center for EUV Astrophysics, University of California, Berkeley to reduce the cost and complexity of science investigations. Their multi-disciplinary plan incorporates both UOT and a complementary technology introduced in this paper, called interactive archives.     

Investigations on gel forming media for use in low gravity bioseparations research.

Microgravity research includes investigations designed to gain insight on methods of separating living cells. During a typical separation certain real-time measurements can be made by optical methods, but some materials must also be subjected to subsequent analyses, sometimes including cultivation of the separated cells. In the absence of on-orbit analytical or fraction collecting procedures, some means is required to "capture" cells after separation. The use of solutions that form gels was therefore investigated as a means of maintaining cells and/or macromolecules in the separated state after two types of simple ground-based experiments. Microgravity electrophoresis experiments were simulated by separating model cell types (rat, chicken, human and rabbit erythrocytes) in a vertical density gradient containing low-conductivity buffer, 1.7%-6.5% Ficoll, 6.8-5.0% sucrose, and 1% SeaPrep low-melting temperature agarose and demonstrating that, upon cooling, a gel formed in the column, and cells could be captured in the positions to which they had migrated. Two-phase extraction experiments were simulated by choosing two-polymer solutions in which phase separation occurs in normal saline at temperatures compatible with cell viability and in which one or both phases form a gel upon cooling. Suitable polymers included commercial agaroses (1-2%), maltodextrin (5-7%) and gelatin (5-20%).     

The evolving microlesion concept.

The "microlesion concept", introduced by D. Grahn in the 1970's, was further explored using published quantitative carcinogenesis data. Laboratory experiments performed by T. C. H. Yang and co-workers and by R. J. M. Fry and co-workers using the Fe ion beam of the Lawrence Berkeley Laboratory Bevalac have resulted in quantitative data on in vitro and in vivo (respectively) carcinogenesis in mouse systems. These data sets were interpreted on the basis of track calculations, and it was found that the action cross section for tumor induction in cultured cells is approximately 0.032 micrometer2 and that it is about 1/1000th as great in mouse Harderian gland cells. This great difference in carcinogenic sensitivity is a reflection of the biological differences between these two highly promoted systems, neither of which may be quantitatively applicable to humans in space.     

An exploration of the effectiveness of artificial mini-magnetospheres as a potential solar storm shelter for long term human space missions

If mankind is to explore the solar system beyond the confines of our Earth and Moon the problem of radiation protection must be addressed. Galactic cosmic rays and highly variable energetic solar particles are an ever-present hazard in interplanetary space.     

Formate as an energy source for microbial metabolism in chemosynthetic zones of hydrothermal ecosystems

Formate, a simple organic acid known to support chemotrophic hyperthermophiles, is found in hot springs of varying temperature and pH. However, it is not yet known how metabolic strategies that use formate could contribute to primary productivity in hydrothermal ecosystems. In an effort to provide a quantitative framework for assessing the role of formate metabolism, concentration data for dissolved formate and many other solutes in samples from Yellowstone hot springs were used, together with data for coexisting gas compositions, to evaluate the overall Gibbs energy for many reactions involving formate oxidation or reduction. The result is the first rigorous thermodynamic assessment of reactions involving formate oxidation to bicarbonate and reduction to methane coupled with various forms of iron, nitrogen, sulfur, hydrogen, and oxygen for hydrothermal ecosystems. We conclude that there are a limited number of reactions that can yield energy through formate reduction, in contrast to numerous formate oxidation reactions that can yield abundant energy for chemosynthetic microorganisms. Because the energy yields are so high, these results challenge the notion that hydrogen is the primary energy source of chemosynthetic microbes in hydrothermal ecosystems.     

Functional weightlessness during clinorotation of cell suspensions.

A clinostat is a device often used in gravitational biology studies. Selecting an appropriate speed of rotation, however, is a frequently debated topic, particularly for suspended cells. In an attempt to define the necessary criteria for determining an acceptable revolution speed, the primary forces governing particle behavior during clinorotation--gravity, diffusion and centrifugation--were mathematically assessed. In support of the theoretical exercise, bacterial growth experiments indicated that results obtained using a clinostat followed trends resembling previous space flight results. It is suspected that this is due, in part at least, to similarly altered external transport processes in each environment.     

Laboratory Tests of the Equivalence Principle at the University of Washington

Equivalence principle tests are probes for new fundamental physics. We have conducted several laboratory experiments to test the equivalence principle with unprecedented precision and generality using a torsion balance placed on a continuously rotating turntable. We present preliminary results comparing the differential accelerations of Ti, Be, and Al towards local masses, the earth and the center of our galaxy.