Signal transduction in T lymphocytes--a comparison of the data from space, the free fall machine and the random positioning machine.

In this paper we discuss the effect of microgravity on T cells and we present the data of studies with two new machines for 0 g simulations. Several experiments in space show that mitogenic T cell activation is lost at 0 g. Immunocytochemistry indicates that such effect is associated with changes of the cytoskeleton. Biochemical studies suggest that the lack of expression of the interleukin-2 receptor is one of the major causes of the loss of activity. In fact, interleukin-2 is the third signal required for full activation. In order to deepen our investigations we are now working with the free-fall machine, FFM, invented by D. Mesland, and with the random positioning machine, RPM, or three-dimensional clinostat, developed by T. Hoson. The FFM produces periods of free-fall lasting approximately 800 ms followed by bounces of 15-30 g lasting 45-60 ms. The RPM eliminates the effect of gravity by rotating biological specimen randomly around two orthogonal axes. While the FFM failed to reproduce the results obtained with T lymphocytes in space, the data from the RPM are in good agreement with those in real microgravity. In fact, the inhibition of the mitotic index in the RPM is 89% compared to static controls. The RPM (as the FFM) can carry markedly larger specimen than the fast rotating clinostat and thus allows to conduct comprehensive studies to select suitable biological objects for further investigations in space.     

The principle of constructing a computation model of equilibrium ribbed stiffened shells in linear displacement-based FEM analysis

The equilibrium between the rods and panels in the ribbed shells is considered based on the total system of structural mechanics equations as applied to a discrete model. Also shown is an approach to solving the problems that permits the stiffness matrix of shear panels to be obtained with regard for the equilibrium along the boundary with rods. The connection with the standard hybrid method of constructing stiffness matrices is indicated. The solutions for separate elements and examples illustrating the approach being proposed are presented.     

Synthesizing the control system of a small gliding unmanned aerial vehicle with high-aspect ratio wing

In this paper, we consider a problem of synthesizing the control system of a small gliding (unpowered) aerial vehicle (GAV) with a high-aspect ratio wing (?? ?? 10). This wing makes it possible to increase significantly the flight range and improve the maneuvering characteristics of the aerial vehicle itself.     

Modeling of helicopter behavior dynamics under the controlling action of the stabilization and control system

A possibility of evaluating dynamic response of a mechanical system to controlling actions of the automatic stabilization and control system is considered on the basis of a numerical experiment. Examples of design analysis are presented for a light-weight helicopter in which an autopilot is part of the control system. The finite element analysis is used in this paper.     

High temperature alters the growth reaction of Pottia protonemata.

Under gravistimulation, dark-grown protonemata of Pottia intermedia revealed negative gravitropism with a growth rate of approximately 28 μm·h⁻¹ at room temperature (20 °C). In 7 days, the protonema formed a bundle of vertically oriented filaments. At an elevated temperature (30 °C), bundles of vertically growing filaments were also formed. However, both filament growth rate and amplitude of the gravicurvature were reduced. Red light (RL) irradiation induced a positive phototropism of most apical protonemal cells at 20 °C. In a following period of darkness, approximately two-thirds of such cells began to grow upward again, recovering their negative gravitropism. RL irradiation at the elevated temperature caused a partial increase in the number of protonemal cells with negative phototropism, but the protonemata did not exhibit negative gravitropism after transfer to darkness. The negative gravitropic reaction was renewed only when protonemata were placed at 20 °C. A dramatic decrease in starch amount in protonemal apical cells, which are sensitive to both gravity and light, occurred at the higher temperature. Such a decrease may be one of the reasons for the inhibition of the protonemal gravireaction at the higher temperature. The observation has a bearing on the starch-statolith theory.     

Effect of clinostating on photosynthetic apparatus of pea plants.

The photosynthetic membrane composition and low temperature fluorescence spectra were analyzed for pea chloroplasts from control and clinostated plants. Clinorotation induces a decrease in the amount of the oligomeric form of the light-harvesting chlorophyll a/b complex (LHCII) and an increase of its monomeric form. Some changes in organization of photosystem 1 (PS1) complex were revealed as well. These changes are in accordance with the variations of fluorescence characteristics and photochemical activity.     

The importance of charged aerosols in the polar mesosphere in connection with Noctilucent Clouds and Polar Mesosphere Summer Echoes

A number of campaigns have been conducted in order to study Polar Mesosphere Summer Echos (PMSE) and Noctilucent Clouds (NLC) in the period 1991–1994. Several sounding rockets have been launched through these layers with measurements being performed on upleg as well as downleg. These include measurements of positive ions and electrons in both ram and wake positions, as well as measurements of charged aerosols in ram on upleg. In this paper we will review these measurements and make a preliminary classification of the data based upon the presence of PMSE and/or NLC. One of the mechanisms responsible for PMSE is the presence of neutral air turbulence in combination with a high Schmidt number. We will briefly discuss this type of echo using in situ rocket data. Differences and similarities of PMSE and NLC as observed both in the Arctic and the Antarctic will be discussed. Observations show that especially PMSE are much more frequent in the Arctic. This may be due to a difference in the water vapour content or the temperature at mesopause heights. Lack of data in the Antarctic makes it difficult to decide which of these two factors are the most important. More measurements, especially co-ordinated in situ and ground-based lidar and radar measurements, are needed to discuss the Arctic and Antarctic similarities and differences in further detail.     

Modelling of genetically engineered microorganisms introduction in closed artificial microcosms.

The possibility of introducing genetically engineered microorganisms (GEM) into simple biotic cycles of laboratory water microcosms was investigated. The survival of the recombinant strain Escherichia coli Z905 (Apr, Lux+) in microcosms depends on the type of model ecosystems. During the absence of algae blooming in the model ecosystem, the part of plasmid-containing cells E. coli decreased fast, and the structure of the plasmid was also modified. In conditions of algae blooming (Ankistrodesmus sp.) an almost total maintenance of plasmid-containing cells was observed in E. coli population. A mathematics model of GEM's behavior in water ecosystems with different level of complexity has been formulated. Mechanisms causing the difference in luminescent exhibition of different species are discussed, and attempts are made to forecast the GEM's behavior in water ecosystems.     

Simulation of the flow around a stationary circular cylinder taking into account laminar-turbulent transition

We consider a problem of a stationary incompressible viscous fluid flow around a flat circular cylinder. In the vicinity of the critical Reynolds number Re _cr a stepwise drop of the cylinder drag takes place, which is called the drag crisis.