Spaceflight and clinorotation cause cytoskeleton and mitochondria changes and increases in apoptosis in cultured cells.

The cytoskeleton is a complex network of fibers that is sensitive to environmental factors including microgravity and altered gravitational forces. Cellular functions such as transport of cell organelles depend on cytoskeletal integrity; regulation of cytoskeletal activity plays a role in cell maintenance, cell division, and apoptosis. Here we report cytoskeletal and mitochondria alterations in cultured human lymphocyte (Jurkat) cells after exposure to spaceflight and in insect cells of Drosophila melanogaster (Schneider S-1) after exposure to conditions created by clinostat rotation. Jurkat cells were flown on the space shuttle in Biorack cassettes while Schneider S-1 cells were exposed to altered gravity forces as produced by clinostat rotation. The effects of both treatments were similar in the different cell types. Fifty percent of cells displayed effects on the microtubule network in both cell lines. Under these experimental conditions mitochondria clustering and morphological alterations of mitochondrial cristae was observed to various degrees after 4 and 48 hours of culture. Jurkat cells underwent cell divisions during exposure to spaceflight but a large number of apoptotic cells was also observed. Similar results were obtained in Schneider S-1 cells cultured under clinostat rotation. Both cell lines displayed mitochondria abnormalities and mitochondria clustering toward one side of the cells which is interpreted to be the result of microtubule disruption and failure of mitochondria transport along microtubules. The number of mitochondria was increased in cells exposed to altered gravity while cristae morphology was severely affected indicating altered mitochondria function. These results show that spaceflight as well as altered gravity produced by clinostat rotation affects microtubule and mitochondria organization and results in increases in apoptosis. Grant numbers: NAG 10-0224, NAG2-985.     

Ambiguity resolution of multiple targets using pulse-Dopplerwaveforms

To cancel clutter, both medium-PRF waveforms which are ambiguous in both range and Doppler and high-PRF waveforms which are ambiguous in range but unambiguous in ambiguities, a previous paper has shown that superior results for a single target can be achieved by using a clustering algorithm. Here, the problem of multiple targets is considered. A maximum likelihood (ML) technique which incorporates the clustering algorithm is developed for the multiple target problem. Simulation results show that four targets which have the same speed but are at different ranges can be resolved by using a medium-PRF waveform and employing the ML resolution technique     

On small active region filaments,fibrils and surges

High resolution Hα images and magnetograms (0.2 arc s) of an active region were obtained in alternating time series at 42 s cadences using the Swedish 1-m Solar Telescope on 2004 August 21. The Hα filtergrams reveal an active region filament and surges consisting of thread-like structures which have widths similar to the widths of chromospheric fibrils, both recorded down to the resolution limit in the best images. All observed structures in the active region appear highly dynamic. Fibrils show counterstreaming strongly resembling the counterstreaming threads in filaments.     

Deceleration and heating constrained footprint of shuttle vehicles

With increasing frequency in shuttle operation, it is of interest to have more than one or two landing fields within the boundary of the reachable area of the reentry vehicle. This boundary, called the footprint, depends on the aerodynamic characteristics of the vehicle and is severely restricted by the deceleration and heating constraints imposed upon the atmospheric reentry trajectory. This paper gives a general assessment of the footprint as a function of various deceleration and heating constraints. The difficulties in the computation of the three-dimensional reentry trajectories with optimal modulation in both the angle-of-attack and the bank angle are alleviated by the following devices: (a) nondimensionalizing of the equations of motion and use of the density as the altitude variable; (b) use of the classical integrals of the motion; (c) transformation of the adjoint variables into physical variables; and (d) spherical rotation of the coordinates.     

Calibration techniques for magnetometers implementing on-board de-spinning algorithms

The Fluxgate Magnetometer experiments on-board the European Space Agency’s four spacecraft Cluster Mission have the capability to store sampled magnetic field vectors in the instrument memory, either as a full resolution ‘event capture’ or as spin-resolution vectors transformed into a non-spinning co-ordinate system (de-spun). The latter capability has ensured a dataset is available which extends the partial orbital coverage achieved during nominal operations in the first years of operation. The on-board de-spin is achieved using a Walsh function with Haar coefficients and allows for up to 27 h additional data per non-coverage interval. A number of commissioning orbits were used to verify the accuracy of data collected by the de-spin mode, whereby individual spacecraft were operated separately in a number of standard normal sampling and de-spin mode combinations. Up to the present time, this data has not been available to the Cluster community. We present results here comparing the performance of the on-board de-spin algorithm versus the normal sampling modes over a number of boundary layer crossings, describe the techniques used for calibration and timeline recovery, and outline the context in which the data may be usable in future studies.     

Wave resistance of rough plane channel walls in the supersonic flow

We propose a method that makes it possible to obtain in the framework of linear approximation the exact formulas for the wave resistance of the channel walls with an arbitrary plane pattern in the first and subsequent interference zones. It is shown by a particular example of the sinusoidal pattern that the pressure wave interference may lead to the positive or negative resistance resonance.     

The Scanning Sky Monitor (SSM) on ASTROSAT

The Scanning Sky Monitor is one of the experiments onboard the ASTROSAT, an Indian multiwavelength astronomy satellite mission. This experiment will detect and monitor X-ray transients in the energy band 2–10 keV. It is similar in design to the ASM on RXTE. It consists of position-sensitive proportional counters with one-dimensional mask. We describe the configuration of the experiment. We also discuss some of the results obtained using a detector which has already been fabricated and tested in our laboratory.     

Information Support for Space Experiments Based on a Numerical-Analytical Theory of Satellite Motion

A software tool for application in calculations of the position and velocity vectors of spacecraft is presented. These vectors are necessary at the stage of processing the data of space experiments and analyzing the results of ground-based observations. A new theory of satellite motion, in a numerical-analytical form, is the basis of this software application. The model of disturbing forces corresponds to recommendations of the International Laser Ranging Service. The reliability of the software tool is confirmed by the results of processing of high-precision laser satellite observations.     

Oxygen Isotopes in the Solar System

The oxygen three-isotope system has major advantages over the two-isotope systems of hydrogen, carbon, and nitrogen because different fractionation laws govern intraplanetary and interplanetary processes. This permits discriminating between solar nebular processes and parent-body processes. Oxygen isotopes also serve as a sensitive natural tracer for meteorite classification. This revised version was published online in August 2006 with corrections to the Cover Date.