Effects of microgravity on c-fos gene expression in osteoblast-like MC3T3-E1 cells.

The paper summarizes the data on proliferation and gravity-related gene expression of osteoblasts that were obtained from an experiment conducted under simulated and real microgravity conditions. Simulated microgravity conditions obtained in a clinostat depress proliferation of both osteoblast-like MC3T3-E1 and HeLa carcinoma cells. This depression of proliferation occurs in a collagen gel culture in which the flow of culture medium by rotation may be reduced. Interestingly, MC3T3-E1 cells which are probably one of target cells to microgravity are more sensitive than the HeLa cells. Simulated microgravity inhibited the epidermal growth factor (EGF)-induced c-fos gene expression in the MC3T3-El cells. To examine in detail the effect of real microgravity on the EGF signal transduction cascade in osteoblasts, MC3T3-E1 cells were cultured in the Cell Culture Experiment Module of the sounding rocket TR-1A6. The EGF-induced c-fos expression in cells was depressed under short-term microgravity conditions in the sounding rocket, while the phosphorylation of mitogen-activated protein kinase (MAPK) was not affected compared with the controls grown on the ground. These results suggest that an action site of microgravity in the signal transduction pathway may be downstream of MAPK.     

Self-perspiration garment for extravehicular activity improves skin cooling effects without raising humidity

Introduction

The current U.S. extravehicular activity (EVA) suit in space includes liquid cooling and ventilation garment (LCVG) to control thermal condition. Tubes knitted in LCVG for flowing water interrupt evaporation of perspiration, and astronauts feel discomfort. In the present study, we hypothesized that a self-perspiration garment would effectively lower the skin temperature without raising humidity in the garment. Thus, we developed and examined the effects of the garment.

Methods

Eight healthy subjects were studied with a cyclic ergometer of 30, 60 90 and 120 W loading for 3 min each. Skin temperature and humidity on the back were measured continuously. Subjects wore and tested three types of garments i.e., a spandex wear without any cooling device (Normal), a simulated LCVG (s-LCVG) or the spandex wear knitted a vinyl tube for flowing water, and the spandex wear with a tube, which flows water and self-perspiration with oozing water for evaporative cooling (SPEC).

Results

All measurements were reached to steady state 2–3 min after the setting. The s-LCVG decreased skin temperature 0.39±0.14 °C during 12 min of cooling. With SPEC, skin temperature did not decrease significantly until 6–9 min after starting the cooling. However, the temperature decreased rapidly and significantly after that, and finally decreased 1.59±0.32 °C. Humidity in the SPEC was significantly lower than that in s-LCVG.

Discussion

SPEC was effective for lowering skin temperature without raising humidity in the garment. The concept is expected to use as a better cooling system during EVA.     

The implementation of game in a 20-day head-down tilting bed rest experiment upon mood status and neurotic levels of rest subjects

This study evaluated the effect of the implementation of game on mental health among participants in a bedrest (BR) experiment. Subjects were 12 healthy males aged 20-26, who participated in a 20-day 6-degrees head-down tilting BR experiment. The participants were asked to complete psychometrical questionnaires before, during, and after the experiment. We entrusted the participants to manage their leisure time and they intended a game in which all of them could take part over the experiment period. The general conversation and light-hearted mood among the subjects continued during the experimental period. Longitudinal data analysis showed that levels of neurosis and mood status did not deteriorate during the experiment, while our previous experiments, which were performed under the same protocol as this study except for the implementation of the game showed a distinct deterioration in psychosocial status. We consider that the implementation of game autonomously contributes to the positive effects on the mental health among the participants.     

Overwintering in the Antarctica as an analog for long term manned spaceflight.

As a part of Polar Psychological Project planned by SCAR group on Antarctic-Space-Related-Human-Factors-Research, a series of psychological tests were conducted on the Japanese wintering-over personnel. The main purpose of the research was to study the behavioral characteristics, such as depression, insomnia, anxiety, and hostility, which might develop during the course of wintering-over period, and it involves a detailed study of the individuals and interpersonal relationship.     

Time variations of cosmic-ray helium isotopes with BESS-Polar I

The Balloon-borne Experiment with a Superconducting Spectrometer (BESS) is configured with a solenoidal superconducting magnet and a suite of precision particle detectors, including time-of-flight hodoscopes based on plastic scintillators, a silica-aerogel Cherenkov detector, and a high resolution tracking system with a central jet-type drift chamber. The charges of incident particles are determined from energy losses in the scintillators. Their magnetic rigidities (momentum/charge) are measured by reconstructing each particle trajectory in the magnetic field, and their velocities are obtained by using the time-of-flight system. Together, these measurements can accurately identify helium isotopes among the incoming cosmic-ray helium nuclei up to energies in the GeV per nucleon region. The BESS-Polar I instrument flew for 8.5 days over Antarctica from December 13th to December 21st, 2004. Its long-duration flight and large geometric acceptance allow the time variations of isotopic fluxes to be studied for the first time. The time variations of helium isotope fluxes are presented here for rigidities from 1.2 to 2.5 GV and results are compared to previously reported proton data and neutron monitor data.     

ETS-II Experiments Part III: Weather Radar System

The weather radar system used in conjunction with Earth-satellite propagation experiments for quantifying rainfall rate in a unit volume, determined by the beamwidth and the pulselength, is described. Firstly, methods for deriving rainfall rate per unit volume are stated in detail with special emphasis on calibration to determine unknown parameters associated with propagation losses in waveguides and the atmosphere. The calibration method is somewhat different from a conventional one but is useful for routine observation. Secondly, in addition to a simple explanation of the hardware of this radar system, the data modes, developed for measuring rainfall rate along the propagation path and radar reflectivity factor in the horizontal and vertical cross sections around the station are described. These data modes are effectively combined by the operational scanning modes for routine observation of propagation experiments, while each data mode can also be operated independently. Examples obtained by each data mode are also given.     

Sounding rocket experiment of bare electrodynamic tether system

An overview of a sounding rocket, S-520-25th, project on space tether technology experiment is presented. The project is prepared by an international research group consisting of Japanese, European, American, and Australian researchers. The sounding rocket will be assembled by the ISAS/JAXA and will be launched in the summer of 2009. The sounding rocket mission includes two engineering experiments and two scientific experiments. These experiments consist of the deployment of bare electrodynamic tape tether in space, a quick ignition test of hollow cathode system in space, the demonstration of bare electrodynamic tether system in space, and the test of the OML (orbital-motion-limit) current collection theory.     

A study of land transformation processes after the atomic bombing damage in Hiroshima

We first analyzed aerial, satellite and surface photographs of Hiroshima taken in 1939, 1945, 1947, 1972, 1980 and 1982, in time sequence. It is rather difficult to recognize traces of damage caused by the atomic bombing from surface level with the few exceptions such as the Atomic Bomb Memorial Dome. However, the ground surface pattern of central Hiroshima analyzed by LANDSAT MSS data shows similarities to damage caused by the atomic bombing and the distribution of residual radioactivities.     

Optical Calibration of Geostationary Satellite Tracking Systems

A precise calibration method for range and angle observation has been developed for eliminating the systematic error of tracking systems, thus improving the accuracy of orbit determination for geostationary satellites. The principle of calibration is based on an orbit determination employing a point of optical angle observation in addition to radio tracking observation, in which we estimate observation bias parameters simultaneously with orbital elements, including the effects of geodetic mismodelings. As shown by an actual calibration experiment in our ground station, orbit determinations is sufficiently accurate that the error of predicting satellite range falls within a few meters at four days after the day of orbit determination.