Molecular analysis of mutagenesis by high LET radiation.

Mutation induction by high linear energy transfer [LET] alpha particles and gamma-rays was scored in the human hamster hybrid [AL] cells. Southern blotting technique was used to analyse the molecular changes in the DNA from both the HGPRT- and S1- mutants. Dose dependent mutagenesis in the AL cells irradiated with the charged particles was higher by almost 20 fold at the S1 than the corresponding HGPRT locus. Southern analysis of the mutants induced by the high LET particles showed mostly multilocus deletion at both the HGPRT and S1 genes.     

Specific effects of irradiance and CO2 concentration doublings on productivity and mineral content in lettuce.

Experiments in growth chambers with controlled atmosphere were performed to compare the effects on the productivity of two treatments stimulating photosynthesis: the doubling of CO2 concentration, the doubling of irradiance; the combining of both was also tested. A large effect of light was noticed: (i) the accumulation of carbon was, contrarily to CO2 effect, amplified within time, and led to the most important dry matter production. (ii) the specific leaf weight was about two-fold increased. (iii) the nitrate content was 2-3 fold less. A significant positive effect of CO2 was detected on the fresh biomass production and the iron content of lettuce. A synergy was observed on dry matter production by the interaction of the two factors.     

Survival and alteration of the plasmid-containing microorganism Escherichia coli Z905/pPHL7 introduced into manmade closed aquatic microcosms.

It has been demonstrated that the transgenic microorganism Escherichia coli Z905/pPHL7 (AprLux+) can exist for a long time at an elevated concentration of mineral salts. The microorganism was introduced into microcosms with sterile brackish water (salinity variable from 21 to 22 g l-1) taken from Lake Shira (Khakasia, Russia). The survival of the microorganism was estimated both by measuring the growth of the colonies on solid nutrient media and by the bioluminescence exhibited by the transgenic strain in samples from the microcosms and in the enrichment culture with the added selective factor-ampicillin (50 micrograms/ml). In the enrichment culture, the bioluminescent signal was registered through the 160-day experiment. It has been shown that in the closed microcosms with brackish water the E. coli strain becomes heterogeneous in its ampicillin resistance. The populations of the transgenic strain were mainly represented by isolates able to persist in the medium containing 50 micrograms/ml, but there were also the cells (about 10%) with the threshold of ampicillin resistance not more than 0.05 micrograms/ml. Thus, it was shown that in the microcosms with brackish water and in the absence of the selective factor the transgenic strain survives and retails the recombinant plasmid.     

The Role of Magnetic Reconnection in CME Acceleration

Observations carried out from the coronagraphs on board space missions (LASCO/SOHO, Solar Maximum and Skylab) and ground-based facilities (HAO/Mauna Loa Observatory) show that coronal mass ejections (CMEs) can be classified into two classes based on their kinematics evolution. These two classes of CMEs are so-called fast and slow CMEs. The fast CME starts with a high initial speed that remains more or less constant; it is also called the constant-speed CME. On the other hand, the slow CME starts with a low initial speed, but shows a gradual acceleration; it is also called the accelerated and slow CME. Low and Zhang [Astrophys. J. 564, L53–L56, 2002] suggested that these two classes of CMEs could be a result of a difference in the initial topology of the magnetic fields associated with the underlying quiescent prominences. A normal prominence magnetic field topology will lead to a fast CME, while an inverse quiescent prominence results in a slow CME, because of the nature of the magnetic reconnection processes. In a recent study given by Wu et al. [Solar Phys. 225, 157–175, 2004], it was shown that an inverse quiescent prominence magnetic topology also could produce a fast CME. In this study, we perform a numerical MHD simulation for CMEs occurring in both normal and inverse quiescent prominence magnetic topology. This study demonstrates three major physical processes responsible for destabilization of these two types of prominence magnetic field topologies that can launch CMEs. These three initiation processes are identical to those used by Wu et al. [Solar Phys. 225, 157–175, 2004]. The simulations show that both fast and slow CMEs can be initiated from these two different types of magnetic topologies. However, the normal quiescent prominence magnetic topology does show the possibility for launching a reconnection island (or secondary O-line) that might be thought of as a “CME’’.     

The asymmetrical growth of otoliths in fish is affected by hypergravity.

Size and asymmetry (size difference between the left and the right side) of inner ear otoliths of larval cichlid fish were determined after a long-term stay at moderate hypergravity conditions (3g; centrifuge), in the course of which the animals completed their ontogenetic development from hatch to freely swimming. Both the normal morphogenetic development as well as the timely onset and gain of performance of the swimming behaviour was not impaired by the experimental conditions. However, both utricular and saccular otoliths (lapilli and sagittae, respectively) were significantly smaller after hyper-g exposure as compared to parallely raised 1g control specimens. The asymmetry of sagittae was significantly increased in the experimental animals, whereas the respective asymmetry con-cerning lapilli was pronouncedly decreased in comparison to the 1g controls. These findings suggest, that the growth and the development of bilateral asymmetry of otoliths is guided by the environmental gravity vector.     

UWB radar for patient monitoring

During the last few years the Moscow Aviation Institute (Russia) and the Industrial Technology Research Institute (Taiwan) have worked jointly on the development of ultrawideband (UWB) medical radars for remote and contactiess monitoring of patients in hospitals. Preliminary results of these works were published in [1]. As of the present, several radars have been produced and tested in real conditions in hospitals in Russia and Taiwan. Some results of these tests are given.     

Multiband echo tomography of Sco X-1

We present preliminary results of a simultaneous X-ray/optical campaign of the prototypical LMXB Sco X-1 at 1–10 Hz time resolution. Lightcurves of the high excitation Bowen/HeII emission lines and a red continuum at λ_c  6000 Å were obtained through narrow interference filters with ULTRACAM, and these were cross-correlated with simultaneous RXTE X-ray lightcurves. We find evidence for correlated variability, in particular when Sco X-1 enters the Flaring branch. The Bowen/HeII lightcurves lag the X-ray lightcurves with a light travel time which is consistent with reprocessing in the companion star while the continuum lightcurves have shorter delays consistent with reprocessing in the accretion disc.     

A root moisture sensor for plants in microgravity.

Development of components for bioregenerative life-support systems is a vital step toward long-term space exploration. The culturing of plants in a microgravity environment may be optimized by the use of appropriate sensors and controllers. This paper describes a sensor developed for determining the amount of fluid (nutrient solution) available on the surface of a porous ceramic nutrient delivery substrate to the roots of conventional crop plants. The sensor is based on the change in thermal capacitance and thermal conductance near the surface as the moisture content changes. The sensor could be employed as a data acquisition and control sensor to support the automated monitoring of plants grown in a microgravity environment.