Can identification of a fourth domain of life be made from sequence data alone, and could it be done on Mars?

A central question in astrobiology is whether life exists elsewhere in the universe. If so, is it related to Earth life? Technologies exist that enable identification of DNA- or RNA-based microbial life directly from environmental samples here on Earth. Such technologies could, in principle, be applied to the search for life elsewhere; indeed, efforts are underway to initiate such a search. However, surveying for nucleic acid-based life on other planets, if attempted, must be carried out with caution, owing to the risk of contamination by Earth-based life. Here we argue that the null hypothesis must be that any DNA discovered and sequenced from samples taken elsewhere in the universe are Earth-based contaminants. Experience from studies of low-biomass ancient DNA demonstrates that some results, by their very nature, will not enable complete rejection of the null hypothesis. In terms of eliminating contamination as an explanation of the data, there may be value in identification of sequences that lie outside the known diversity of the three domains of life. We therefore have examined whether a fourth domain could be readily identified from environmental DNA sequence data alone. We concluded that, even on Earth, this would be far from trivial, and we illustrate this point by way of examples drawn from the literature. Overall, our conclusions do not bode well for planned PCR-based surveys for life on Mars, and we argue that other independent biosignatures will be essential in corroborating any claims for the presence of life based on nucleic acid sequences.     

Biomarker and stable isotope characterization of coastal pond-derived organic matter, McMurdo Dry Valleys, Antarctica

Small coastal ponds that contain photosynthetic microbial mat communities represent an extreme environment where a potentially significant source of labile organic carbon can be found within the McMurdo Dry Valleys, Antarctica. To distinguish coastal pond-derived organic matter from other sources of organic matter in the Dry Valleys, bulk organic carbon, nitrogen, and sulfur isotope signatures and phospholipid fatty acid (PLFA) profiles of benthic microbial mats located at two sites--Hjorth Hill coast and Garwood Valley--were investigated. The average isotope values at Hjorth Hill coast and Garwood Valley are, respectively, -10.9 per thousand and -10.2 per thousand for delta(13) C, 3.7 per thousand and -1.3 per thousand for delta(15)N, and 8.1 per thousand and 16.7 per thousand for delta(34)S. Microbial mats from all ponds are dominated by monounsaturated PLFAs (indicative of Gram-negative bacteria) and polyunsaturated PLFAs (indicative of microeukaryotes). Biomarkers specific to aerobic prokaryotes, eukaryotes, and photoautotrophic microeukaryotes, as well as sulfur-reducing bacteria, are present in all samples. Benthic mats at Garwood Valley are thicker and more laminated, have a higher biomass, and have a greater carbon and nitrogen content, which suggests greater productivity than mats at Hjorth Hill coast. Greater productivity is supported, as well, by higher dissolved oxygen contents likely derived from heightened photosynthetic productivity. More productivity at Garwood Valley likely results from a larger influx of terrestrial surface waters together with a concomitant nutrient loading.     

Bacterial community in ancient Siberian permafrost as characterized by culture and culture-independent methods

The microbial composition of ancient permafrost sediments from the Kolyma lowland of Northeast Eurasia was examined through culture and culture-independent approaches. These sediments have been continuously frozen for 5,000 to 2-3 million years. A total of 265 Bacteria 16S rRNA gene sequences were amplified from the permafrost total-community genomic DNA and screened by amplified ribosomal 16S rRNA restriction analysis. Members of three major lineages were found: gamma-Proteobacteria (mostly Xanthomonadaceae), Actinobacteria, and Firmicutes. We also determined partial 16S rRNA gene sequences of 49 isolates from a collection of 462 aerobes isolated from these sediments. The bacteria included Actinomycetales (Arthrobacter and Microbacteriaceae); followed by the Firmicutes (Exiguobacterium and Planomicrobium); the Bacteroidetes (Flavobacterium); the gamma-Proteobacteria (Psychrobacter); and the alpha-Proteobacteria (Sphingomonas). Both culture and culture-independent approaches showed the presence of high and low G+C Gram-positive bacteria and gamma-Proteobacteria. Some of the 16S rRNA gene sequences of environmental clones matched those of Arthrobacter isolates. Two-thirds of the isolates grew at -2.5 degrees C, indicating that they are psychroactive, and all are closely related to phylogenetic groups with strains from other cold environments, mostly commonly from Antarctica. The culturable and non-culturable microorganisms found in the terrestrial permafrost provide a prototype for possible life on the cryogenic planets of the Solar System.     

Spectral properties of Lake Superior banded iron formation: application to Martian hematite deposits

Several locations have been identified on Mars that expose bulk, coarsely crystalline gray hematite. These deposits have been interpreted as being sedimentary and formed in aqueous environments. Lake Superior Type (LST) banded iron formation (BIF) was investigated as a spectral and possible process analog to these deposits. In northern Michigan, LST BIF formed in a sedimentary, continental shelf or shallow basin environment under stable tectonic conditions, and the oxide facies contains gray, crystalline hematite. These deposits are Proterozoic in age and contain microfossils associated with the early diversification of life on Earth. Samples of the hematite-bearing oxide facies, as well as the carbonate facies, were collected and analyzed for their spectral and geochemical characteristics. Sample spectra were measured in the visible, near-infrared, and thermal infrared for comparison with remote and in situ spectra obtained at Mars. Thin section analysis, as well as X-ray diffraction and scanning electron microscopy measurements, were performed to determine detailed geochemistry. There is no evidence for BIF at Opportunity's Meridiani landing site, and the results of this work will provide useful data for determining whether BIFs exist elsewhere on Mars and are, thus, relevant to current and future Mars exploration missions.     

The Physical Foundation of Forecasting Disturbances in the Geospace From Solar Disk Characteristics

The five main types of antisunward propagating energetic fluxes (particles and emission) may be thought of as well established to date, the effects of which lead to a particilar character of disturbance in the near-terrestrial environment (the Earth's magnetosphere, ionosphere and atmosphere). The strongest global restructuring of the magnetosphere and ionosphere is caused by fluxes of relatively dense n of 1-70 cm-3 at the Earth's orbit) Solar Wind (SW) quasi-neutral, low-energy (E ＜ 10 keV) plasma which cause magnetospheric and ionospheric storms lasting 24 hours or longer. For that reason, main attention is given to their study at the initial stage of research. The physical essence of the method of predicting disturbances in the near-terrestrial space environment, the amplitude of which can be expressed in, for example, the Kp index units, involves:(1) identifying all the most geo-effective SW streams of type, (2) determing their sources on the solar disk,and (3) quantifying the correlations between the characteristics of their solar sources with a maximum value of the Kp-index that is caused by the concerned type of SW stream. Semi-phenomenological relations have been obtained, which relate parameters of type SW stream sources to characteristics of geomagnetic storms:storm commencement, the time at which the storm intensity reaches its maximum values, the storm duration,as well as to the storm amplitude expressed in terms of geomagnetic indeces.      

Transport modeling of energetic electrons in the inner magnetosphere with synchrotron energy losses

The resulting L-distributions and energy spectra of energetic magnetospheric electrons obtained from numerical solution of the radiation belt transport equation with and without accounting for electron synchrotron energy losses are compared. It is demonstrated that synchrotron losses play an important role in formation of the space and energetic distributions of electrons in the inner magnetosphere.     

Parametric models for helicopter identification using ANN

An artificial neural network (ANN) based helicopter identification system is proposed. The feature vectors are based on both the tonal and the broadband spectrum of the helicopter signal, ANN pattern classifiers are trained using various parametric spectral representation techniques. Specifically, linear prediction, reflection coefficients, cepstrum, and line spectral frequencies (LSF) are compared in terms of recognition accuracy and robustness against additive noise. Finally, an 8-helicopter ANN classifier is evaluated. It is also shown that the classifier performance is dramatically improved if it is trained using both clean data and data corrupted with additive noise.     

Radio plasma imager simulations and measurements

The Radio Plasma Imager (RPI) will be the first-of-its kind instrument designed to use radio wave sounding techniques to perform repetitive remote sensing measurements of electron number density (N _e) structures and the dynamics of the magnetosphere and plasmasphere. RPI will fly on the Imager for Magnetopause-to-Aurora Global Exploration (IMAGE) mission to be launched early in the year 2000. The design of the RPI is based on recent advances in radio transmitter and receiver design and modern digital processing techniques perfected for ground-based ionospheric sounding over the last two decades. Free-space electromagnetic waves transmitted by the RPI located in the low-density magnetospheric cavity will be reflected at distant plasma cutoffs. The location and characteristics of the plasma at those remote reflection points can then be derived from measurements of the echo amplitude, phase, delay time, frequency, polarization, Doppler shift, and echo direction. The 500 m tip-to-tip X and Y (spin plane) antennas and 20 m Z axis antenna on RPI will be used to measures echoes coming from distances of several R _E. RPI will operate at frequencies between 3 kHz to 3 MHz and will provide quantitative N _e values from 10^–1 to 10⁵ cm^–3. Ray tracing calculations, combined with specific radio imager instrument characteristics, enables simulations of RPI measurements. These simulations have been performed throughout an IMAGE orbit and under different model magnetospheric conditions. They dramatically show that radio sounding can be used quite successfully to measure a wealth of magnetospheric phenomena such as magnetopause boundary motions and plasmapause dynamics. The radio imaging technique will provide a truly exciting opportunity to study global magnetospheric dynamics in a way that was never before possible.     

The Radio Plasma Imager investigation on the IMAGE spacecraft

Radio plasma imaging uses total reflection of electromagnetic waves from plasmas whose plasma frequencies equal the radio sounding frequency and whose electron density gradients are parallel to the wave normals. The Radio Plasma Imager (RPI) has two orthogonal 500-m long dipole antennas in the spin plane for near omni-directional transmission. The third antenna is a 20-m dipole along the spin axis. Echoes from the magnetopause, plasmasphere and cusp will be received with the three orthogonal antennas, allowing the determination of their angle-of-arrival. Thus it will be possible to create image fragments of the reflecting density structures. The instrument can execute a large variety of programmable measuring options at frequencies between 3 kHz and 3 MHz. Tuning of the transmit antennas provides optimum power transfer from the 10 W transmitter to the antennas. The instrument can operate in three active sounding modes: (1) remote sounding to probe magnetospheric boundaries, (2) local (relaxation) sounding to probe the local plasma frequency and scalar magnetic field, and (3) whistler stimulation sounding. In addition, there is a passive mode to record natural emissions, and to determine the local electron density, the scalar magnetic field, and temperature by using a thermal noise spectroscopy technique.