Changes in gravitational force induce alterations in gene expression that can be monitored in the live, developing zebrafish heart.

Little is known about the effect of microgravity on gene expression, particularly in vivo during embryonic development. Using transgenic zebrafish that express the gfp gene under the influence of a beta-actin promoter, we examined the affect of simulated-microgravity on GFP expression in the heart. Zebrafish embryos, at the 18-20 somite-stage, were exposed to simulated-microgravity for 24 hours. The intensity of GFP fluorescence associated with the heart was then determined using fluorescence microscopy. Our measurements indicated that simulated-microgravity induced a 23.9% increase in GFP-associated fluorescence in the heart. In contrast, the caudal notochord showed a 17.5% increase and the embryo as a whole showed only an 8.5% increase in GFP-associated fluorescence. This suggests that there are specific effects on the heart causing the more dramatic increase. These studies indicate that microgravity can influence gene expression and demonstrate the usefulness of this in vivo model of 'reporter-gene' expression for studying the effects of microgravity.     

Parathyroid hormone-related protein is a gravisensor in lung and bone cell biology.

Parathyroid Hormone-related Protein (PTHrP) has been shown to be essential for the development and homeostatic regulation of lung and bone. Since both lung and bone structure and function are affected by microgravity, we hypothesized that 0 x g down-regulates PTHrP signaling. To test this hypothesis, we suspended lung and bone cells in the simulated microgravity environment of a Rotating Wall Vessel Bioreactor, which simulates microgravity, for up to 72 hours. During the first 8 hours of exposure to simulated 0 x g, PTHrP expression fell precipitously, decreasing by 80-90%; during the subsequent 64 hours, PTHrP expression remained at this newly established level of expression. PTHrP production decreased from 12 pg/ml/hour to 1 pg/ml/hour in culture medium from microgravity-exposed cells. The cells were then recultured at unit gravity for 24 hours, and PTHrP expression and production returned to normal levels. Based on these findings, we have obtained bones from rats flown in space for 2 weeks (Mission STS-58, SL-2). Analysis of PTHrP expression by femurs and tibias from these animals (n=5) revealed that PTHrP expression was 60% lower than in bones from control ground-based rats. Interestingly, there were no differences in PTHrP expression by parietal bone from space-exposed versus ground-based animals, indicating that the effect of weightlessness on PTHrP expression is due to the unweighting of weight-bearing bones. This finding is consistent with other studies of microgravity-induced osteoporosis. The loss of the PTHrP signaling mechanism may be corrected using chemical agents that up-regulate this pathway. In conclusion, PTHrP represents a stretch-sensitive paracrine signaling mechanism that may sense gravity.     

Microgravity particle research on the space station: the Gas-Grain Simulation Facility.

In the gravitational field on Earth, the large settling rate of micron-sized particles and the effects of gravity-induced convection prohibit many interesting studies of phenomena such as coagulation, collisions, and mutual interactions of droplets, dust grains and other particles. Examples of exobiology experiments involving these phenomena are the simulation of organic aerosol formation in Titan's atmosphere, studies of the role of comets in prebiotic chemical evolution, and simulations of carbon grain interactions in various astrophysical environments. The Gas-Grain Simulation Facility (GGSF) is a proposed Earth-orbital laboratory that will allow present ground-based experimental programs which study processes involving small particles and weak interactions to be extended to a new domain. Physics issues that scientists wishing to propose GGSF experiments must consider are reviewed in this paper. Specifically, coagulation, motion in gases and vacua, and wall deposition of particles in a microgravity environment are discussed.     

Spending without results: lessons from the space station program

The US spent all of the funds originally estimated for the initial development of its orbital space station without producing any significant amount of flight hardware. This article shows how a project with large design costs and significant “non-prime” outlays can quickly deplete program funds. The authors recount the way in which budgetary politics, congressional micro-management, and technological risk conspired to produce this result.     

Defense Meteorological Satellite Program

The DMSP is a total satellite system composed of spacecraft with meteorological sensors, an earthbased command and control network, fixed and mobile user stations, and a communication network linking the various segments together.     

Multiband echo tomography of Sco X-1

We present preliminary results of a simultaneous X-ray/optical campaign of the prototypical LMXB Sco X-1 at 1–10 Hz time resolution. Lightcurves of the high excitation Bowen/HeII emission lines and a red continuum at λ_c  6000 Å were obtained through narrow interference filters with ULTRACAM, and these were cross-correlated with simultaneous RXTE X-ray lightcurves. We find evidence for correlated variability, in particular when Sco X-1 enters the Flaring branch. The Bowen/HeII lightcurves lag the X-ray lightcurves with a light travel time which is consistent with reprocessing in the companion star while the continuum lightcurves have shorter delays consistent with reprocessing in the accretion disc.     

Navigating towards the future: transitioning from terrestrial radio navigation to satellite navigation and airborne surveillance

This paper presents a proposal for transitioning from terrestrial-based navigation aids to implementing satellite and airborne surveillance as the primary navigation means. The transition occurs through several steps. First, the installation and use of modern navigation and surveillance equipment is mandated by the regulatory organizations. The installations should take place in a sequenced fashion to allow time for companies to absorb the initial cost. Next, the existing network of terrestrial navigation aids is down-sized leaving only the areas of heaviest use in service. At this point, the global positioning system (GPS) will be deemed the primary method of terrestrial and oceanic travel. Finally, terrestrial navigation stations will be available around airports and the remaining stations will be put in a standby condition for use in the event of a national emergency. This paper will discuss the security benefits and examples of cost savings through implementation of these steps.