HZE-dosimetry on MIR92, IML-1 and D-2

Nuclear track detectors were used to measure the integral Linear Energy Transfer (LET) spectra above 1 GeV per cm water behind the complex material shielding inside a spacecraft. The measurements are compared with predictions of the contribution of high charge, high energy HZE particles of the galactic cosmic radiation taking into account the influence of solar and geomagnetic modulation and shielding by matter.     

Distribution of calmodulin in corn seedlings: Immunocytochemical localization in coleoptiles and root apices

Immunofluorescence techniques have been used to study the distribution of calmodulin in several tissues in etiolated corn ( , var. Bear Hybrid) seedlings. Uniform staining was seen in the background cytoplasm of most cell types. Cell walls and vacuoles were not stained. In coleoptile mesophyll cells the nucleoplasm of most nuclei was stained as was the stroma of most amyloplasts. The lumen border of mature tracheary elements in coleoptiles also stained. In the rootcap the most intensely stained regions were the cytoplasms of columella cells and of the outermost cells enmeshed in the layer of secreted slime. Nuclei in the rootcap cells did not stain distinctly, but those in all cell types of the root meristem did. Also in the root meristem, the cytoplasm of metaxylem elements stained brightly. These results are compared and contrasted with previous data on the localization of calmodulin in pea root apices and epicotyls and discussed in relation to current hypotheses on mechanisms of gravitropism.     

The potential of the lichen symbiosis to cope with the extreme conditions of outer space II: germination capacity of lichen ascospores in response to simulated space conditions.

Complementary to the already well-studied microorganisms, lichens, symbiotic organisms of the mycobiont (fungi) and the photobiont (algae), were used as "model systems" in which to examine the ecological potential to resist to extreme environments of outer space. Ascospores (sexual propagules of the mycobiont) of the lichens Fulgensia bracteata, Xanthoria elegans and Xanthoria parietina were exposed to selected space-simulating conditions (up to 16 h of space vacuum at 10(-3) Pa and UV radiation at 160 nm < or = lambda < or = 400 nm), while embedded in the lichen fruiting bodies. After exposure, the ascospores were discharged and their viability was tested as germination capacity on different culture media including those containing Mars regolith simulant. It was found that (i) the germination rate on media containing Mars regolith simulant was as high as on other mineral-containing media, (ii) if enclosed in the ascocarps, the ascospores survived the vacuum exposure, the UV-irradiation as well as the combined treatment of vacuum and UV to a high degree. In general, 50 % or more viable spores were recovered, with ascospores of X. elegans showing the highest survival. It is suggested that ascospores inside the ascocarps are well protected by the anatomical structure, the gelatinous layer and the pigments (parietin and carotene) against the space parameters tested.     

Changes in vacuolation in the root apex cells of soybean seedlings in microgravity.

Changes in the vacuolation in root apex cells of soybean (Glycine max L. [Merr.]) seedlings grown in microgravity were investigated. Spaceflight and ground control seedlings were grown in the absence or presence of KMnO4 (to remove ethylene) for 6 days. After landing, in order to study of cell ultrastructure and subcellular free calcium ion distribution, seedling root apices were fixed in 2.5% (w/v) glutaraldehyde in 0.1 M cacodylate buffer and 2% (w/v) glutaraldehyde, 2.5% (w/v) formaldehyde, 2% (w/v) potassium antimonate K[Sb(OH)6] in 0.1 M K2HPO4 buffer with an osmolarity (calculated theoretically) of 0.45 and 1.26 osmol. The concentrations of ethylene in all spaceflight canisters were significantly higher than in the ground control canisters. Seedling growth was reduced in the spaceflight-exposed plants. Additionally, the spaceflight-exposed plants exhibited progressive vacuolation in the root apex cells, particularly in the columella cells, to a greater degree than the ground controls. Plasmolysis was observed in columella cells of spaceflight roots fixed in solutions with relatively high osmolarity (1.26 osmol). The appearance of plasmolysis permitted the evaluation of the water status of cells. The water potential of the spaceflight cells was higher than the surrounding fixative solution. A decrease in osmotic potential and/or an increase in turgor potential may have induced increases in cell water potential. However, the plasmolysed (i.e. non-turgid) cells implied that increases in water potential were accompanied with a decrease in osmotic potential. In such cells changes in vacuolation may have been involved to maintain turgor pressure or may have been a result of intensification of other vacuolar functions like digestion and storage.     

Thymine photoproduct formation and inactivation of intact spores of Bacillus subtilis irradiated with short wavelength UV (200-300nm) at atmospheric pressure and in vacuo.

Vacuum exposure renders the survival of spores of Bacillus subtilis approximately five times more sensitive to ultraviolet light irradiation than exposure under atmospheric conditions. The photoproduct formation in spores irradiated under ultrahigh vacuum (UHV) conditions is compared to the photoproduct formation in spores irradiated at atmospheric pressure. Compared to irradiation at atmospheric pressure, where only the "spore photoproduct" 5-thyminyl-5,6-dihydrothymine (TDHT) can be detected, two additional photoproducts, known as the c,s and t,s isomers of thymine dimer (T<>T) are produced in vacuo. The spectral efficiencies for photoproduct formation in spores under atmospheric and vacuum conditions are compared. Since there is no increased formation of TDHT after irradiation in vacuum, TDHT cannot be made responsible for the observed vacuum effect. "Vacuum specific" photoproducts may cause a synergistic response of spores to the simultaneous action of ultraviolet light (UV) and UHV. Three different mechanisms are discussed for the enhanced sensitivity of B. subtilis spores to UV radiation in vacuum. The experiments described contribute valuable research information on the chance for survival of microorganisms in outer space.     

Chemistry of the interstellar medium--an evolutionary dead end?

An analysis of the experimental data available and of the present theoretical concepts shows that even the initial physicochemical chemical precellular stages of biological evolution are impossible in the interstellar medium, while biomonomers possibly formed on asteroids and comets might have participated after transportation to the Earth in the final stages of the origin of the first precellular biological structures and then in the first living cells.     

An approach to the detection of microbe life in planetary environments through charge-coupled devices

The use of charge-coupled devices is suggested as a means for detecting growth of micro-organism colonies. The accuracy of the method is determined by channel width and the sensitivity by the signal/noise ratio. The method was tested on a dense nutrient medium, which is to be considered more suitable to micro-organisms of the dry Martian soil than the water solution of nutrients employed in the Viking's strategy.     

Galileo Photopolarimeter/Radiometer experiment

The Photopolarimeter/Radiometer (PPR) is a remote sensing instrument on the Galileo Orbiter designed to measure the degree of linear polarization and the intensity of reflected sunlight in ten spectral channels between 410 and 945 nm to determine the physical properties of Jovian clouds and aerosols, and to characterize the texture and microstructure of satellite surfaces. The PPR also measures thermal radiation in five spectral bands between 15 and 100 m to sense the upper tropospheric temperature structure. Two additional channels which measure spectrally integrated solar and solar plus thermal radiation are used to determine the planetary radiation budget components. The PPR photopolarimetric measurements utilize previously flown technology for high-precision polarimetry using a calcite Wollaston prism and two silicon photodiodes to enable simultaneous detection of the two orthogonal polarization components. The PPR radiometry measurements are made with a lithium tantalate pyroelectric detector utilizing a unique arrangement of radiometric stops and a scene/space chopper blade to enable a warm instrument to sense accurately the much colder scene temperatures.     

The plasma instrumentation for the Galileo Mission

The plasma instrumentation (PLS) for the Galileo Mission comprises a nested set of four spherical-plate electrostatic analyzers and three miniature, magnetic mass spectrometers. The three-dimensional velocity distributions of positive ions and electrons, separately, are determined for the energy-per-unit charge (E/Q) range of 0.9 V to 52 kV. A large fraction of the 4-steradian solid angle for charged particle velocity vectors is sampled by means of the fan-shaped field-of-view of 160°, multiple sensors, and the rotation of the spacecraft spinning section. The fields-of-view of the three mass spectrometers are respectively directed perpendicular and nearly parallel and anti-parallel to the spin axis of the spacecraft. These mass spectrometers are used to identify the composition of the positive ion plasmas, e.g., H⁺, O⁺, Na⁺, and S⁺, in the Jovian magnetosphere. The energy range of these three mass spectrometers is dependent upon the species. The maximum temporal resolutions of the instrument for determining the energy (E/Q) spectra of charged particles and mass (M/Q) composition of positive ion plasmas are 0.5 s. Three-dimensional velocity distributions of electrons and positive ions require a minimum sampling time of 20 s, which is slightly longer than the spacecraft rotation period. The two instrument microprocessors provide the capability of inflight implementation of operational modes by ground-command that are tailored for specific plasma regimes, e.g., magnetosheath, plasma sheet, cold and hot tori, and satellite wakes, and that can be improved upon as acquired knowledge increases during the tour of the Jovian magnetosphere. Because the instrument is specifically designed for measurements in the environs of Jupiter with the advantages of previous surveys with the Voyager spacecraft, first determinations of many plasma phenomena can be expected. These observational objectives include field-aligned currents, three-dimensional ion bulk flows, pickup ions from the Galilean satellites, the spatial distribution of plasmas throughout most of the magnetosphere and including the magnetotail, and ion and electron flows to and from the Jovian ionosphere.