Comparative metagenomics of two microbial mats at cuatro ciénegas basin I: ancient lessons on how to cope with an environment under severe nutrient stress

Abstract The Cuatro Ciénegas Basin (CCB) is an oasis in the desert of Mexico characterized by low phosphorus availability and by its great diversity of microbial mats. We compared the metagenomes of two aquatic microbial mats from the CCB with different nutrient limitations. We observed that the red mat was P-limited and dominated by Pseudomonas, while the green mat was N-limited and had higher species richness, with Proteobacteria and Cyanobacteria as the most abundant phyla. From their gene content, we deduced that both mats were very metabolically diverse despite their use of different strategies to cope with their respective environments. The red mat was found to be mostly heterotrophic, while the green mat was more autotrophic. The red mat had a higher number of transporters in general, including transporters of cellobiose and osmoprotectants. We suggest that generalists with plastic genomes dominate the red mat, while specialists with minimal genomes dominate the green mat. Nutrient limitation was a common scenario on the early planet; despite this, biogeochemical cycles were performed, and as a result the planet changed. The metagenomes of microbial mats from the CCB show the different strategies a community can use to cope with oligotrophy and persist. Key Words: Microbial mats-Metagenomics-Metabolism. Astrobiology 12, 648-658.     

Bacterial communities and the nitrogen cycle in the gypsum soils of cuatro ciénegas basin, coahuila: a Mars analogue

Abstract The OMEGA/Mars Express hyperspectral imager identified gypsum at several sites on Mars in 2005. These minerals constitute a direct record of past aqueous activity and are important with regard to the search of extraterrestrial life. Gale Crater was chosen as Mars Science Laboratory Curiosity's landing site because it is rich in gypsum, as are some desert soils of the Cuatro Ciénegas Basin (CCB) (Chihuahuan Desert, Mexico). The gypsum of the CCB, which is overlain by minimal carbonate deposits, was the product of magmatic activity that occurred under the Tethys Sea. To examine this Mars analogue, we retrieved gypsum-rich soil samples from two contrasting sites with different humidity in the CCB. To characterize the site, we obtained nutrient data and analyzed the genes related to the N cycle (nifH, nirS, and nirK) and the bacterial community composition by using 16S rRNA clone libraries. As expected, the soil content for almost all measured forms of carbon, nitrogen, and phosphorus were higher at the more humid site than at the drier site. What was unexpected is the presence of a rich and divergent community at both sites, with higher taxonomic diversity at the humid site and almost no taxonomic overlap. Our results suggest that the gypsum-rich soils of the CCB host a unique microbial ecosystem that includes novel microbial assemblies. Key Words: Cuatro Ciénegas Basin-Gale Crater-Gypsum soil microbial diversity-Molecular ecology-Nitrogen cycle. Astrobiology 12, 699-709.     

Oriented platform for the VEGA-probe to the Halley comet,position detection,working program

Two deep-space probes of the Venera type for the encounter with Halley's comet are being prepared in the Intercosmos program. 150 kg of scientific equipment will be onboard each sonde, including two TV cameras and two spectrometers. These instruments need precise orientation during the fly-by of the comet. For this purpose a stabilised platform is being developed in Czechoslovakia which will be installed onboard both probes and will be able to point to the target with precision 5 minutes of arc and maximum angular velocity 1°/second. A system of position detectors and the working program during the start and the fly-by are described.     

Dynamic and figure parameters of Venus and Mars

Parameters of the best-fitting tri-axial ellipsoids representing external equipotential surfaces of Venus and Mars have been determined from satellite data. The dynamic consequence of the equatorial flattening of Venus has been discussed from the point of view of the s.c. synodic resonance rotation. The major gravitational anomalies of Venus have been interpreted, space locations and magnitudes of anomalous masses determined and their contribution to the second zonal Stokes' constant in the gravitational potential computed. The conclusions were done: The figure of the aphroditoid is strange even if there is a relatively small polar flattening; an equatorial “disc” of Venus is enormous. Recent space data do not support hypothesis that the Earth controls the spin of Venus.