Early and late damages induced by heavy charged particle irradiation in embryonic tissue of Arabidopsis seeds.

Early and late effects of accelerated heavy ions (HZE) on the embryonic tissue of Arabidopsis thaliana seeds were investigated seeing that initial cells of the plant eumeristems resemble the original cells of animal and human tissues with continuous cell proliferation. The endpoints measured were lethality and tumorization in the M1-generation for early effects and embryonic lethality in the M2-generation for late effects. The biological endpoints are plotted as functions of the physical parameters of the irradiation i.e. ion fluence (p/cm2), dose (Gray), charge Z and linear energy transfer (LET). The results presented contribute to the estimation of the principles of biological HZE effects and thus may help to develop a unified theory which could explain the whole sequence from physical and chemical reactions to biological responses connected with heavy ion radiation. Additionally, the data of this paper may be used for the discussion of the quality factor for heavy ion irradiation needed for space missions and for HZE-application in radio-therapy by use of accelerators (UNILAC, (SIS/ESR), BEVALAC).     

Bio-markers and the search for extinct life on Mars.

Geologic and climatologic studies suggest that conditions on early Mars were similar to early Earth. Because life on Earth is believed to have originated during this early period (3.5 billion years ago), the Martian environment could have also been conducive to the origin of life. To investigate this possibility we must first define the attributes of an early Martian biota. Then, specific geographic locations on Mars must be chosen where life may have occurred (i.e. areas which had long standing water), and within these distinct locations search for key signatures or bio-markers of a possible extinct Martian biota. Some of the key signatures or bio-markers indicative of past biological activity on Earth may be applicable to Mars including: reduced carbon and nitrogen compounds, CO3(2-), SO4(2-), NO3-, NO2- [correction of NO2(2)], Mg, Mn, Fe, and certain other metals, and the isotopic ratios of C, N and S. However, we must also be able to distinguish abiotic from biologic origins for these bio-markers. For example, abiotically fixed N2 would form deposits of NO3- and NO2-, whereas biological processes would have reduced these to ammonium containing compounds, N2O, or N2, which would then be released to the atmosphere. A fully equipped Mars Rover might be able to perform analyses to measure most of these biomarkers while on the Martian surface.     

A demonstration of sub meter GPS orbit determination and highprecision user positioning

High-accuracy orbits have been determined for satellites of the Global Positioning System (GPS), with submeter orbit accuracy demonstrated for two well-tracked satellites. Baselines of up to 2000 km in North America determined with the GPS orbits shows daily repeatability of 0.3-2 parts in 10⁸ and agree with very long baseline interferometry (VLBI) solutions at the level of 1.5 parts in 10 ⁸. Tests used to assess orbit accuracy include orbit repeatability from independent data sets, orbit prediction, ground baseline determination, and formal errors. One satellite tracked for eight hours each day shows RMS errors below 1 m even when predicted more than three days outside of a 1-week data arc. These results demonstrate the powerful relative positioning capability available from differential GPS tracking. Baselines have also been estimated between Florida and sites in the Caribbean region over 1000 km away, with daily repeatability of 1-4 parts in 10⁸. The best orbit estimation strategies included data arcs of 1-2 weeks, process noise models for tropospheric fluctuations, combined processing of GPS carrier phase and pseudorange data, and estimation of GPS solar pressure coefficients     

Integrated CIS VLF/Omega receiver design

The design of an integrated Russian-VLF/Omega receiver implemented on a TMS320C30-microprocessor-based SPECTRUM plug-in board installed in a PC-compatible portable computer is presented. The system also requires an external antenna, pre-amp, and frequency reference. The SPECTRUM board digitizes the RF signal to 16 b and then digitally mixes with the sines and cosines of the three Russian frequencies plus 10.2, 11-1/3, and 13.6 kHz. The mixer outputs are low-pass filtered, and the comb filters are implemented for the respective epochs. The computer accesses and processes the comb filter outputs, calculating and logging signal phase and amplitude. The design allows for easy future expansion to include unique and VLF communication frequencies     

Re-analysis of the cosmic ray ground level enhancement of 4 May 1960.

The relativistic solar particle event of 4 May 1960, resulting in a cosmic ray ground level enhancement, occurred well before modern analysis techniques were available. We have located surviving data from 23 neutron monitors and have used these to estimate the spectrum, mean arrival direction and particle pitch angle distribution as the event progressed. We find that the apparent particle arrival direction was at equatorial latitudes, over northern South America, in contrast to contemporary analyses that proposed it to be over North America. Our modified power law spectra are broadly consistent with earlier results. Data from stations above sea level need to be corrected for altitude using a two-attenuation length technique. The standard method involves comparison of data from two relatively close stations at significantly different altitude. We have shown that this method may be unreliable in cases, such as this, of quite sharp anisotropy.     

Programmable ultrawideband signal generator for electromagnetic susceptibility testing

Under Phase I of the Defense Advanced Research Projects Agency (DARPA) Networking in Extreme Environments (NETEX) initiative, Multispectral Solutions, Inc. (MSSI) was tasked with the development of a general-purpose, ultrawideband hardware simulator capable of reproducing a wide variety of ultrawideband (UWB) waveforms. The simulator, with UWB outputs from baseband through millimeter wave, was to be used for the purpose of electromagnetic susceptibility testing of legacy military radio, radar, and positioning systems. The ultimate goal of this portion of the Phase I program was the quantitative determination of those UWB parameters (e.g., frequency, power, pulse rate, pulse shape, dithering, etc.) which had the least impact on the operational performance of legacy designs. This describes the development of the MSSI NETEX UWB simulator (BFP1000).     

Gravitropism in cut flower stalks of snapdragon.

The negative gravitropic response of cut flower stalks is a complex multistep process that requires the participation of various cellular components acting in succession or in parallel. The process was particularly characterized in snapdragon (Antirrhinum majus L.) spikes with regard to (1) gravity stimulus perception associated with amyloplast reorientation; (2) stimulus transduction mediated through differential changes in the level, action and related genes of auxin and ethylene and their possible interaction; (3) stimulus response associated with differential growth leading to stalk curvature; (4) involvement of cytosolic calcium and actin cytoskeleton. Results show that the gravity-induced amyloplast reorientation, differential over-expression of two early auxin responsive genes and asymmetrical distribution of free IAA are early events in the bending process. These precede the asymmetrical ethylene production and differential stem growth, which was derived from initial shrinkage of the upper stem side and a subsequent elongation of the lower stem side. Results obtained with various calcium- and cytoskeleton-related agents indicate that cytosolic calcium and actin filaments may play essential roles in gravitropism-related processes of cut flower stalks. Therefore, modulators of these two physiological mediators may serve as means for controlling any undesired gravitropic bending.     

Main characteristics of biological components of developing life support system observed during the experiments aboard orbital complex MIR.

Since 1990, the orbital complex MIR has witnessed several incubator experiments for determination of spaceflight effects on embryogenesis of Japanese quail. First viable chicks who had completed the whole embryological cycle in MIR microgravity hatched out in 1990; it became clear that newborns would not be able to adapt to microgravity unaided. There were 8 successful incubations of chicks in the period from 1990 to 1999. In 1995-1997 the MIR-NASA space science program united Russian and US investigators. As a result, experiments Greenhouse-1 and 2 were performed with an effort to grow super dwarf wheat from seed to seed, and experiment Greenhouse-3 aimed at receiving two successive generations of Brassica rapa. But results of these experiments could not be used for definitive conclusions concerning effects of spaceflight on plant ontogenesis and, therefore, experiments Greenhouse-4 and 5 were staged within the framework of the Russian national space program. The experiments finally yielded wheat seeds. Some of the seeds was left on the space station and, being planted, gave viable seedlings which, in their turn, produced the second crop of space seeds.     

Effects of slow clinorotation on lipid contents and proton permeability of thylakoid membranes of pea chloroplasts.

Photochemical characteristics and lipid composition of thylakoid membranes from 12 day-old pea leaves that were exposed to slow clino-rotation were examined and compared with a vertical control. Proton permeability of thylakoid membranes was estimated from light-induced proton uptake (delta H+) and post-illumination proton efflux in chloroplast suspensions. The delta pH magnitude was calculated from the level of light-induced quenching of 9-aminoacridine fluorescence. Proton permeability of thylakoid membranes increased during exposure to clino-rotation. When subsequently transferred to darkness, proton efflux increased almost 2-fold in clinorotated leaves. The results were compared with data on pigment and polar lipid composition of photosynthetic membranes in clino-rotated and control plants. It was concluded that both the increase of proton permeability and the decrease of polar lipid content in chloroplasts were induced by clino-rotation.     

Development and application of the Interactive Planetary Image Processing System (IPIPS) in support of remote sensing studies at Imperial College

In this paper we describe the Interactive Planetary Image Processing System (IPIPS) which was developed originally for studies of planetary meteorology and oceanography and is now used to support remote sensing studies in all areas of earth sciences. The computing machinery, the image display systems and the programming that unites them into an interactive research and analysis tool are described. We present some results from our recent research activities and also outline the role of IPIPS in the Imperial College and University of London teaching programme.